LOCUS       sequence-445013-        3310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Chen B, Cepko CL
  TITLE     HDAC4 regulates neuronal survival in normal and diseased retinas.
  JOURNAL   Science. 2009 Jan 9. 323(5911):256-9.
  PUBMED    19131628
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Jan 9. 323(5911):256-9."
COMMENT     Sequence Label: PBS/pU6- HIF1 alpha RNAi plasmid 1
FEATURES             Location/Qualifiers
     source          1..3310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..32
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     24..41
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     25..41
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..103
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        100..413
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     332..352
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(494..510)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(547..567)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(547..565)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(586..602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(586..602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(599..621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    610..626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(634..640,641..658,659..664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    679..700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(817..834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(988..1576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1068..1087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1747..2538,2539..2607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2370..2389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2608..2712)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2738..3193)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2875..2896)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3087..3106
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
       61 cactataggg cgaattgggt acccgctcta gaactagtgg atccgacgcc gccatctcta
      121 ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc ccctgccccg
      181 gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag
      241 ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat ttctataaga
      301 gatacaaata ctaaattatt attttaaaaa acagcacaaa aggaaactca ccctaactgt
      361 aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt gtttggagct
      421 tgctcatcag ttgccagtgt gctgtcctgg caactgatga gcaagctcct ttttgaattc
      481 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
      541 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
      601 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      661 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2701 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     2761 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2821 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2881 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2941 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3001 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3061 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3121 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3181 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3241 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3301 tgggtaacgc
//
