LOCUS       sequence-445024-        8882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696),
            but the overlap between GvpS and GvpK has been resolved..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'
FEATURES             Location/Qualifiers
     source          1..8882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1588..1607)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1589..1607)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1844..2035
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2029..2051)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2137..2276
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2192..2211
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2291..2308)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2462..3050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2542..2561)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3081..3120)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(3985..4076)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(4143..4190)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4186..4204
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(8065..8082)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
ORIGIN
        1 tctccagagc ggtttcgcac gtttgctacg agcgttacaa ttgtgcgcgc ttcatcttct
       61 tctacgtccg ttttctccgt taaaatcgct gtctctctgt cgatgtcttt ataatcaata
      121 aacatggtaa ttaacctgtc gagaagcgca tcttgcgtat catatacgcc ggcatactca
      181 gctggattgc ttgtgaagat gacgcggaaa tcgggatgca cgcgcacaaa aggatcggtc
      241 atttttactc catacagcgg cagcacgcct tcttctaata tcgatagaaa gatattattc
      301 gtcgcaggct tagaacgagt aaattcgtcg taaatcagcg tatagccatt ttttacagct
      361 tcaagcaatc ggccatcctg ccagttttca ctcacctgtt catctttttt atagacagaa
      421 cgaacgtact ggtcgattac ttttttgctc gtgtatcccg taaaatcgcc aattaaatct
      481 ttgttgttga gctcgtgatt cccgtgcatc agcattacag gacgctttct ctttttagca
      541 agcgctcgcg ctaaagaggt tttgcctccg ccggcaggac ctgtaaaatg aatggaatag
      601 ccggatttta aatagctcag cgctcttgaa agaacctctt ttgtctcgtc atcttgtata
      661 aaagctccac tgcctttttt ccttttgtct gttaagacgg tcatttttac gttcctcctg
      721 ccatcttatc gtttatgctt caatgccaat ggcgcttcta tatctgacgt cgagtcgttt
      781 gaatgaaatg acttcttttt ctttatttac aaagcactcg tacgttccga gcatttcatc
      841 tttggcgtat tttttcatat attctcgttc ttcaatgact tcaacaataa ctctccaacc
      901 ttcatcttca gtagcttcca ccgaggtaat tttatgagga ggagctacgt gttcaccgaa
      961 aaagtcgttc acggcttgca taattttttt aatttccatt tttagtagcc tccaatagtt
     1021 agatctaaaa gctggaacca ccctcaaaaa aggaaggttc cataccactt catattaaat
     1081 actaaaacgc ggctgccctt cacttgaatt tgaacgttca ggaagaccgt tttcttctac
     1141 gtcgtcacga agaagcccta ctgcttctgc atagcgtaac catgtatcaa cactggcaat
     1201 aaccactcgc gcttcaatcg ttaaaatttc aattcctaca acagaaactc ttgcaaaagc
     1261 atcaataaca attcctttat ctaaaatacg gtcaatgact tctgctaaac ttgaactatt
     1321 agtacttttt tgaatagaca tatctcattt ctcctttcgc ttatgatccc attgttttta
     1381 aatctgtaga tgatttaata tcattagaac ttttaatctt agtagagctt ttaatatccg
     1441 ttgctttttt aatctgattt acgcctttta ttttccgctt attttttgac ttctctttat
     1501 cgtctgaaga agaaactttt tcttgaaagt tttctccagc ttctttcaca tcgctaagct
     1561 atctctagag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgcgggatc
     1621 gatgcatggt gccaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     1681 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     1741 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     1801 tcattacccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
     1861 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     1921 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     1981 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2041 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     2101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     2161 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     2221 caccattgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     2281 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2341 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2401 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2461 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2521 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2581 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2641 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2701 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2761 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2821 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2881 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2941 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3001 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3061 tgatcttttc taccggtccg tccaaaaaaa aaggctccaa aaggagcctt taattgtatc
     3121 ggtttaccaa tgcttaatca gtgaagcccc tatttcagca atctgtctat ttcgttcgtc
     3181 catagttgcc tgactacccg tcgtgtagat aactacgata cgggaaggct taccatctgg
     3241 tccaagtgcg gcaatgatac cccgtgaacc acgctctcct gccccagatt tatcagcaat
     3301 aaaccagcca gcaggaagtg ccgaacgcag tagtggtcct gcaactttat ccgcttcaag
     3361 ccagtctatt aattgttgcc gtgaagccag agtaagtagt tcgccagtta atagtttgcg
     3421 taatgttgtt gccattgccg caggcattgt ggtgtctcgc tcgtcgtttg gtatggcttc
     3481 attcagttct ggttcccaac ggtcaaggcg agttacatgg tctcccatat tgtgcaaaaa
     3541 agcggttaat tccttcggtc caccgatggt tgtcagaagt aagttcgccg cagtgttatc
     3601 actcatcgtt atggcggcac tacataattc tcttaccgtc attccgtccg taaggtgctt
     3661 ttctgtcact ggggagtatt caaccaagtc attctgcgaa tagtgtattc gtcgtccgag
     3721 ttgctcttgc cccgcatcta cacgggataa tactgctcca catagcagaa ctttgaaagt
     3781 gctcatcatt ggaaaacgct cttcagggcg aaaactctca aggattttac cgctattcaa
     3841 gtccagttct atgtatccca ctcgtgcccc caattggtcc tctgcgtctt ttactttcac
     3901 gagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3961 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4021 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4081 ggaattaaaa aaaagcccgc tcattaggcg ggctactagt cggatatagt tcctcctttc
     4141 agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca
     4201 gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg atctctcgag
     4261 acgcgttcgc gaatccggac tgcagcaagc agaagcaaat gtgacggaag tcgcctatca
     4321 agtagggttt aatagtacaa gctattttat taatcagttt cgaaaagaaa tgaagatgac
     4381 tccgctcatg tatcgaaaaa agaagctgca catctcatca catcactaaa aaacggcggg
     4441 gcaatcaccc cgccgttttt tatgaactct ttttactcgt tgattttgca tcagaaatct
     4501 ttcctaagaa aaacccgtct acttctgcta ttttccctct ccaaaagatt ttgcctttag
     4561 aaggagtaga tttactgtct ccacagtaaa tctttgtatt tttcaaatga ataaaatggg
     4621 cttctgcttc tccgtttgat tcgctcgctt cgcttgctaa agagaattgt tcgcttagcg
     4681 cctgagccac ttcactgcct tcttcaaacg tttcgcttaa gtaatcaaag tattcttttg
     4741 ctgaaatcat ggtaccggaa atcacagcgc cgtttacatt taatgaaata tcaagtgaaa
     4801 agccgtgttt attagaagct ttcacaaagt attcaagcgt attgtcttta gttgaaaaag
     4861 aagggcctgt actcatgtct ttctttcctc ctttacgtct taggctgttg tatcgtcttc
     4921 actagaaaga gatgttgctt catcatcttc ttcgtccgtg tttttctttg cttttgtatt
     4981 ttttgatttt ttagctgttg acttttttgg tttcgcttcc tttttttctt cttcgctttc
     5041 atcttcgtct tcttcttctg tttttacttt tttatttttt ttgtttgagc gagaatcttc
     5101 ctcttcttcc tcgctgtctt cgtcactttc actttcttct tcctcagctt cttttttatc
     5161 ctttttacgt ggcttagatg tttcttcttc gttttcatcg tcctgctcgt tttcatcgtt
     5221 ttcatcgttc tcatcgttct cttcttcgtc ggaatctgta tcttccgctt cttcgtcttg
     5281 atttttattg aggctaagct caacaagcat gttcattttt tcttcaagct gtgataagcg
     5341 atcttgaaga gtttgatttt cttcttttaa ctcgtcgtac tcttgaacgt tatcgtcttc
     5401 tgtttcgcta ctagaagaat ttacgttttc ttcatcatct tttgatttat ctttatcttt
     5461 tttaaacaag cttcccgcag atgttttcag gctggccacg ctgctttttg atttttcttt
     5521 tacctttgtt cctaaatcag atgctttgct ttttaattcg tctgtatcaa tgcggtcaag
     5581 gagacttttt cgattttcag gagtcgatac atatccaatc gttgcaccga tgatgcctcc
     5641 tgctaccgct cgtttgattg gcccgctgct tgttgttttg cttgcttttg aaccgttctt
     5701 ttcttttgaa ccgttttccg catttttatt ttccttgttt tctgcctgtg tgttatctaa
     5761 ttttgtttca gttgccattg taaatccctc cattttttaa gtagcgtaca gtttttaacg
     5821 tttcgtttct attttttctt caagcgtttt taaccgctca tgcagcattt tattttcttc
     5881 ttcgagcgct ttgttattgg ctccttttga actgagatac ggatcatttt cccaccagtc
     5941 catgccgatt tcttttgcct tatcaaccga agccacaatc aagcggatct ttatcgttaa
     6001 tagctcgaca tctgcaattc ctacggtgat gtcccccgct ataacgactc ctttatccaa
     6061 aatcttttcg agcacatcta caatcgtact tgactgcata ttatgttcga ctgccatact
     6121 gttcctccta ctgaccgctt aaagcaggct gcctagcggg ccaagatcaa tatttaaatc
     6181 ttctgcatcc agaccgaaca cctctttcaa ctcgtccatt ttttcttcta agttcattag
     6241 tgcaattcct aagttttcaa tttgttcgtc cgtcaacgtt ccaccctcca cccgcctcat
     6301 ggcatgacgt tcaacgattt gcctcaatag ctcaataact gtcatcacaa gctgcgctaa
     6361 gccttgttca gcttgatcag gatccaagtg gattcgtcca tttgcttggc tgaccggttg
     6421 catctattaa ttcctcctgg actgtcacgc ttaaccgctc ggaattcgag aaagcatacg
     6481 actgctcgca agggtcacga acctagaggg ttcgtccact tactaggctg gcccgtagcg
     6541 tccatcagct cttctttttg tttatcaaat tgttcagaag tgattggttt gtgatttcct
     6601 tcttttgctt gcacaagcgt ttcaaccgaa gaaataagca cccgcaaatc caaatacact
     6661 aaatcgacgc cagctatgga aatgattaag tctcctttaa tggcgactcc tttatctaaa
     6721 atgacatcta aaatatcaat aagagcaata tctttattct ccatggattg tttaagagac
     6781 ataatgttaa ttcctcactt tacgcaaagc tcgagaaatg atagggcggc catggtcccg
     6841 tcacttcaaa cttccagcct ttattttcaa gcctttgctg aagctcttct atttcgttta
     6901 cgaacttagt aatctgcaga gacgggagaa gaaacacgct gttccacgcc atctgttcag
     6961 ctgcgccagt tacgtctttg ctccaattct ttttaacaga gtcataaagc gataattctt
     7021 ttagcttgtc atgtatctct tcacacactt tgtttttgtg aagctctaat tctttttcaa
     7081 tcagctgatc tatttttttc ttttcaaaaa actgtcttcc tggtgataag tgactaattt
     7141 cttgtttttt cgctttcacg ctttcattcg tttcgctgat tcctttttta agctctgtat
     7201 catcacagta aattttcaca ttccactctt catttccttg aagcagcgtc agtgaattct
     7261 ctatcttttc tttgttaatc tcaatagctg cctgcagact ttcttcacct ttataaatgg
     7321 tgcaaaattt taatggaatg atcgtaaatt cttcgtacaa agcggctacc gtttcgtgat
     7381 gatgaaatgc tttttcctgc agccagctca tatcctgctc cattttttct tgaatcactt
     7441 tttctgaata ggtgtcagca tccagcttag aaactacagc cgtcacctga tcaaacgcaa
     7501 ttgggtacag tgaatgttct ccgtcaaacc ccttataaga tggaaacggc tctatggctg
     7561 ctgcttcttt tgttggaatt aaaccgtata aatacagtaa ttctcccatc taggattcct
     7621 catttctttt ttgtgttagc tcttcccatt gttcaatttc tctgcgtttc gcaatttcgt
     7681 acctcattaa caattcatct tctttttctt gaaacgcttc ttctggaatt tcaccaagct
     7741 caaacatcat ttgaagctga atgagctttt gctgaatcgt cggaaggtca tatagctgtt
     7801 tatcagcttc ttcctgtact ttttcgccga ttttcactac aaggttaatg ggtgcggtta
     7861 ctaatttgtg aagcacgtta tttctcttct acttttaggc gaatgttcac aaaattataa
     7921 gcaggccaag gaccgctgta atgaaaatcg gctttgtctt tccagttttc atgcgcttca
     7981 tttacttttt catcaaactt cgcttcatct tctcggttaa ttaagaaaga cgcgtttaaa
     8041 agcatcgttt cgcccgttgg ctcatttgct tttgctgctt ccgctgcttc ttcaagcgga
     8101 gaaaacacat ctgtcttgac ttctttttgc agggaagtaa acatcttttg agccattcct
     8161 ccaagttgaa tacgctcata ataaccggct gcttctgatt ttccttttac ggatgctgat
     8221 actttctcaa gttcaggatt ttcgtttact tttttctcaa gccattcttt tttcccaatt
     8281 acttttaaac cgacttcaat ttttcctttg atcgctggaa acagcttttc aaactgcgga
     8341 taaaggtttt ccaaaagaac ttttacgtct tcttttgatt tgaatacatt cccaaagctg
     8401 attggaataa ccgtatcgtt cttgtccata atcgctgcta ctgcgttttg atgcattaat
     8461 aaattttggc gattaggatg ataaattttc atcggtactt cagctgctac catagccgcg
     8521 tctttgtagc gaatcaaaaa tgtttcagct tttgttcctt ctacttccac ggcgccaaat
     8581 tcttcgtctt tatccgtttg aatggcgcta aaaatataaa tacctgtttc gtttgtttca
     8641 ctcatgtttt caagctcctt tacttttctt cagtgtctat attcttacat tcttctaaaa
     8701 tgattttttc ggctttgctt tttgcatttt cttcatccaa acatttggta agcggatgat
     8761 gcaaaatatc gatacataac gtttgaaaat cttcgtcact tccatcgata ggaatgtctt
     8821 gctgcgtggc aagggtagcg atcataagcg aagcccgcag gctaagtccg ctgctgtttt
     8881 cg
//
