LOCUS       sequence-445030-       12702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-3_FR Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-Tc1-3_FR-10 kb
FEATURES             Location/Qualifiers
     source          1..12702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1414..1495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1451..1470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3038..3061)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3061..3070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3085..4263)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3094..4032
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3117..3137)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    4309..4533
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4522..4539
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(4561..5274,5275..5277,5278..5280))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4564..5274,5275..5277,5278..5280))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4606..4627)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4955..4974
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5214..5235
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5281..5337)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5344..5943)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5424..5444)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      5940..5949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5963..6462)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6011..6030)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6404..6427
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(6891..6908)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6891..6907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6917..6935)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      6970..6999
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      7021..7608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7509..7528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7620..7647)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7671..7862)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(7863..8654,8655..8723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAELGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8486..8505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8724..8826)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(8827..8858)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     8910..8927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8939..8955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8939..8955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9247..9641
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 tttctcaagg tccaacttat gttttcttaa tttttaaaaa aatcttgacc attctccact
       61 ctctaaaata atccacagtg agagaaacat tcttttcccc catcccataa atacctctat
      121 taaatatgga aaatctgggc atggtgtctc acacctgtaa tcccagcact ttgggaggct
      181 gaggtgggtg gactgcttgg agctcaggag ttcaagacca tcttggacaa catggtgata
      241 ccctgcctct acaaaaagta caaaaattag cctggcatgg tggtgtgcac ctgtaatccc
      301 agctattagg gtggctgagg caggagaatt gcttgaaccc gggaggcgga ggttgcagtg
      361 agctgagatc gtgccactgc actccagcct gggggacaga gcacattata attaactgtt
      421 attttttact tggactcttg tggggaataa gatacatgtt ttattcttat ttatgattca
      481 agcactgaaa atagtgttta gcatccagca ggtgcttcaa aaccatttgc tgaatgatta
      541 ctatactttt tacaagctca gctccctcta tcccttccag catcctcatc tctgattaaa
      601 taagcttcag tttttcctta gttcctgtta catttctgtg tgtctccatt agtgacctcc
      661 catagtccaa gcatgagcag ttctggccag gcccctgtcg gggtcagtgc cccacccccg
      721 ccttctggtt ctgtgtaacc ttctaagcaa accttctggc tcaagcacag caatgctgag
      781 tcatgatgag tcatgctgag gcttagggtg tgtgcccaga tgttctcagc ctagagtgat
      841 gactcctatc tgggtcccca gcaggatgct tacagggcag atggcaaaaa aaaggagaag
      901 ctgaccacct gactaaaact ccacctcaaa cggcatcata aagaaaatgg atgcctgaga
      961 cagaatgtga catattctag aatatattat ttcctgaata tatatatata tatatataca
     1021 tatacgtata tatatatata tatatatatt tgttgttatc aattgccata gaatgattag
     1081 ttattgtgaa tcaaatattt atcttgcagg tggcctctat acctagaagc ggcagaatca
     1141 ggctttatta atacatgtgt atagattttt aggatctata cacatgtatt aatatgaaac
     1201 aaggatatgg aagaggaagg catgaaaaca ggaaaagaaa acaaaccttg tttgccattt
     1261 taaggcaccc ctggacagct aggtggcaaa aggaagcttc cctttggtct ccccctctta
     1321 agtaccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1381 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1501 agttgtggtt tgtccaaact caatcgatgc gagggggcag ggcctgcatg tgaagggcgt
     1561 cgtaggtgtc cttggtggct gtactgagac cctggtaaag gccatcgtgc cccttgcccc
     1621 tccggcgctc gcctttcatc ccaatctcac tgtaggcctc cgccatctta tctttctgca
     1681 gttcattgta caggccttcc tgagggttct tccttctcgg ctttcccccc atctcagggt
     1741 cccggccacg tctcttgtcc aaaacatcgt actcctctct tcgtcctaga ttgagctcgt
     1801 tatagagctg gttctggccc tgctggtacg cgggggcgtc tgcgctcctg ctgaacttca
     1861 ctctggagcg ataggctgcg aagtcgcgtg gtggggcata gggctggtaa tgcttgcggg
     1921 tgggcccggg gcggcgggga gtcatgttca tgtagtcact gtgcaggagc ctgctcctct
     1981 tactcctcac ccagaaaata ataaaggcca ctgttactag caagctatag caagccagga
     2041 ctcccccaac caccaccagc acccaaaaat cacaggcgaa gtccagcccc ctcgtgtgca
     2101 ctgcgccccc cgccgctggc cggcacgcct ctgggcgcag ggacaggggc tgcgacgcga
     2161 tggtgggcgc cggtgttggt ggtcgcggcg ctggcgtcgt ggtcagcact gtcagttgcg
     2221 tgcctccacc gaaaacagcg gctgaactat gccaaatcat acagtaataa tcggcctcgt
     2281 cctcggacct gagacctgag atgagcagca cacccgcatt ggcgcttgcg tctttagatc
     2341 cactaaacct tgatggaact ccagatccct gctgcttatc tgagtcgctc ttgtaattga
     2401 gaaggtattg gggaggagaa cctggttttt gctgatacca gtagatcctg taaggcccta
     2461 cattgattcc tgacctcagt gtacacgtca aagaagcgga ggcaccgggg cttgcactga
     2521 gggaggatga ttgcgtgaga acaggttggc tacccccccc tccggacccg cccccaccgg
     2581 aacctccacc gcctgagccc aggatcccac tggacacggt gacggttgta ccttgtcccc
     2641 aaacgtccat accatagtag taagtcatca taccccgcgc acaatagtaa acagctgtgt
     2701 cttcgggagt tacactatta agctgaagag agaactggtt tttggaagta tctggattta
     2761 tagacatcct gcttttcaca cttacagcat aatcattata ccatttactg cggtagtatg
     2821 ttctaccgag ccattccaac cctcgactgg gggactgcct gatccaattc catgtcgcgg
     2881 agttactact cactgaatca cctgatatgg cacaggtaag actaagagtc tgggaaggag
     2941 tgaccagacc ggggccagat tgttggagtt gcacttgcgg cctggcggcg tggagcagca
     3001 aggccagcgg caggagcaag gcggtcactg gtaaggcctt atcgtcatcg tctttgtaat
     3061 ccatggtggc gaccggtgga tccctcacga cacctgaaat ggaagaaaaa aactttgaac
     3121 cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga
     3181 gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa
     3241 ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa
     3301 ctaatcgagg tgcctggacg gcgcccggta ctcagtggag tcacatgaag cgacggctga
     3361 ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg
     3421 cgtcctccat tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc
     3481 aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag
     3541 gccaggcacc agagcaggcc ggcgagcttg agactacccc cgtccgattc tcggtggccg
     3601 cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc
     3661 cgcggcccca aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc
     3721 ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc
     3781 gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc
     3841 aggccaggcc tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact
     3901 ctcccaccca cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga
     3961 agtaattcaa ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca
     4021 cacggcactt acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac
     4081 tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac
     4141 atcactttcc cagtttaccc cgcgccacct tctctaggca ccgggtcaat tgccgacccc
     4201 tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga
     4261 gcctcacgtt aattaaccaa ggtacccatt ggcggccgcc ctcagaagcc atagagccca
     4321 ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
     4381 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
     4441 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     4501 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gcttaattaa
     4561 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     4621 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
     4681 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     4741 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     4801 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     4861 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     4921 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     4981 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     5041 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     5101 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     5161 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     5221 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     5281 tggcccggga ttctcttcga catcccctgc ttgtttcaac agggagaagt tagtggcatc
     5341 gatggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc
     5401 ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc
     5461 caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct
     5521 gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg
     5581 ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga
     5641 accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct
     5701 ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat
     5761 gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg
     5821 gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg
     5881 tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtccc
     5941 catggtggcg ttggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg
     6001 cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg
     6061 ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag
     6121 cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc
     6181 attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc
     6241 acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag
     6301 aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg
     6361 aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag
     6421 cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat tgctagcacc
     6481 ggtggtggag agtttggaat ctgtctgatt gtctgattct gtgaacagtt gtcttacata
     6541 caggtgatta gttagacctg gtgtcttcaa ttcaggtaac aagttgtttg ggagcgtcta
     6601 actggtctgt gaaagccaga actcctaatg catactaggg gatcaaatac ttatttccct
     6661 caatgaaaaa caaataaatt aaaataaatt ctttaaagtt attttcttga ttttcttttt
     6721 gatattctgt ctctccatgt tagaatacat ctaccattaa aagtatagaa tgatcatgtc
     6781 tttattagtg gacaaaccaa caaaatcagc aagggatcaa atacttttta ctctcactgt
     6841 aggtaccaga gacgcaagac actgcggatc gagacgcatt gcgtggttcg actggccgtc
     6901 gttttacaac gaaagggcct cgtgatacgc ctattgactc cagcgtaact ggactgcaat
     6961 caactcactg gctcaccttc acgggtgggc ctttcttcgg tagaaaatca aaggatcttc
     7021 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7081 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaggt aactggcttc
     7141 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7201 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7261 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7321 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7381 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7441 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7501 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7561 gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7621 gcagaaaggc ccacccgaag gtgagccagg tgattacatt tgggccctca tcagaggttt
     7681 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     7741 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     7801 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     7861 acttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7921 atagttgcct gactccccgt cgtgtagata actacgatgc gggagggctt accatctggc
     7981 cccagttctg caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata
     8041 aaccagccag ccgggagggc cgagcgcaga agtgatcctg caactttatc cgcctccatc
     8101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8281 gcggttagct ccttcggtcc tccgatcgtt gccagaagta agttggccgc agtgttatca
     8341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt gagatgcttt
     8401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8521 ctcatcattg gaaaacgttc ttcggggcgt aaactctcaa ggatcttacc gctgttgaga
     8581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8701 acacggaaat gttgaatact cattttagct tccttagctc ctgaaaatct cgataactca
     8761 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     8821 cgatcaagtc aaaagcctcc ggtcggaggc ttttgacttt ctgctatgga ggtcaggtat
     8881 gatttaaatg gtcagtaacg accgagcgca gcgagtcagt gagcgaggaa gcggcacaca
     8941 ggaaacagct atgaccgtct cggttggcag tgactccgtc tctgggccct acagtgagag
     9001 taaaaagtat ttgatccctt gctgattttg ttggtttgtc cactaataaa gacatgatca
     9061 ttctatactt ttaatggtag atgtattcta acatggagag acagaatatc aaaaagaaaa
     9121 tccagaaaat aactttaaag aatttatttt aatttatttg tttttcattg agggtaataa
     9181 gtatttgatc cctctatcca aaagcacctc gagggcccat gaggacagct aaaacaataa
     9241 gtaatgtaaa atacagcata gcaaaacttt aacctccaaa tcaagcctct acttgaatcc
     9301 ttttctgagg gatgaataag gcataggcat caggggctgt tgccaatgtg cattagctgt
     9361 ttgcagcctc accttctttc atggagttta agatatagtg tattttccca aggtttgaac
     9421 tagctcttca tttctttatg ttttaaatgc actgacctcc cacattccct ttttagtaaa
     9481 atattcagaa ataatttaaa tacatcattg caatgaaaat aaatgttttt tattaggcag
     9541 aatccagatg ctcaaggccc ttcataatat cccccagttt agtagttgga cttagggaac
     9601 aaaggaacct ttaatagaaa ttggacagca agaaagcgag cttagtgata cttgtgggcc
     9661 agggcattag ccacaccagc caccactttc tgataggcag cctgcactgg tggggtgaat
     9721 tctttgccaa agtgatgggc cagcacacag accagcacgt tgcccaggag ctgtgggagg
     9781 aagataagag gtatgaacat gattagcaaa agggcctagc ttggactcag aataatccag
     9841 ccttatccca accataaaat aaaagcagaa tggtagctgg attgtagctg ctattagcaa
     9901 tatgaaacct cttacatcag ttacaattta tatgcagaaa tatttatatg cagaaatatt
     9961 gctattgcct taacccagaa attatcactg ttattcttta gaatggtgca aagaggcatg
    10021 atacattgta tcattattgc cctgaaagaa agagattagg gaaagtatta gaaataagat
    10081 aaacaaaaaa gtatattaaa agaagaaagc attttttaaa attacaaatg caaaattacc
    10141 ctgatttggt caatatgtgt acacatatta aaacattaca ctttaaccca taaatatgta
    10201 taatgattat gtatcaatta aaaataaaag aaaataaagt agggagatta tgaatatgca
    10261 aataagcaca catatattcc aaatagtaat gtactaggca gactgtgtaa agtttttttt
    10321 taagttactt aatgtatctc agagatattt ccttttgtta tacacaatgt taaggcatta
    10381 agtataatag taaaaattgc ggagaagaaa aaaaaagaaa gcaagaatta aacaaaagaa
    10441 aacaattgtt atgaacagca aataaaagaa actaaaacga tcctgagact tccacactga
    10501 tgcaatcatt cgtctgtttc ccattctaaa ctgtaccctg ttacttctcc ccttcctatg
    10561 acatgaactt aaccatagaa aagaagggga aagaaaacat caagggtccc atagactcac
    10621 cctgaagttc tcaggatcca cgtgcagctt gtcacagtgc agctcactca gtgtggcaaa
    10681 ggtgcccttg aggttgtcca ggtgagccag gccatcacta aaggcaccga gcactttctt
    10741 gccatgagcc ttcaccttag ggttgcccat aacagcatca ggagtggaca gatccccaaa
    10801 ggactcaaag aacctctggg tccaagggta gaccaccagc agcctaaggg tgggaaaata
    10861 gaccaatagg cagagagagt cagtgcctat cagaaaccca agagtcttct ctgtctccac
    10921 atgcccagtt tctattggtc tccttaaacc tgtcttgtaa ccttgatacc aacctgccca
    10981 gggcctcacc accaacttca tccacgttca ccttgcccca cagggcagta acggcagact
    11041 tctcctcagg agtcaggtgc accatggtgt ctgtttgagg ttgctagtga acacagttgt
    11101 gtcagaagca aatgtaagca atagatggct ctgccctgac ttttatgccc agccctggct
    11161 cctgccctcc ctgctcctgg gagtagattg gccaacccta gggtgtggct ccacagggtg
    11221 aggtctaagt gatgacagcc gtacctgtcc ttggctcttc tggcactggc ttaggagttg
    11281 gacttcaaac cctcagccct ccctctaaga tatatctctt ggccccatac catcagtaca
    11341 aattgctact aaaaacatcc tcctttgcaa gtgtatttac gcttctttga gaaacatctt
    11401 cttcgttagt ggcctgcccc tcattcccac tttaatatcc agaatcacta taagaagaat
    11461 ataataagag gaataactct tattataggt aagggaaaat taagaggcat acgtgatggg
    11521 atgagtaaga gaggagaggg aaggattaat ggatgataaa atctactact atttgttgag
    11581 accttttata gtctaatcaa ttttgctatt gttttccatc ctcacgctaa ctccataaaa
    11641 aaacactatt attatcttta ttttgccatg acaagactga gctcagaaga gtcaagcatt
    11701 tgcctaaggt cggacatgtc agaggcagtg ccagacctat gtgagactct gcagctactg
    11761 ctcatgggcc ctgtgctgca ctgatgagga ggatcagatg gatggggcaa tgaagcaaag
    11821 gaatcattct gtggataaag gagacagcca tgaagaagtc tatgactgta aatttgggag
    11881 caggagtctc taaggacttg gatttcaagg aattttgact cagcaaacac aagaccctca
    11941 cggtgacttt gcgagctggt gtgccagatg tgtctatcag aggttccagg gagggtgggg
    12001 tggggtcagg gctggccacc agctatcagg gcccagatgg gttataggct ggcaggctca
    12061 gataggtggt taggtcaggt tggtggtgct gggtggagtc catgactccc aggagccagg
    12121 agagatagac catgagtaga gggcagacat gggaaaggtg ggggaggcac agcatagcag
    12181 catttttcat tctactacta catgggactg ctcccctata cccccagcta ggggcaagtg
    12241 ccttgactcc tatgttttca ggatcatcat ctataaagta agagtaataa ttgtgtctat
    12301 ctcatagggt tattatgagg atcaaaggag atgcacactc tctggaccag tggcctaaca
    12361 gttcaggaca gagctatggg cttcctatgt atgggtcagt ggtctcaatg tagcaggcaa
    12421 gttccagaag atagcatcaa ccactgttag agatatactg ccagtctcag agcctgatgt
    12481 taatttagca atgggctggg accctcctcc agtagaacct tctaaccagc tgctgcagtc
    12541 aaagtcgaat gcagctggtt agactttttt taatgaaagc ttgtaccagt ggggcctcta
    12601 agactaagtc actctgtctc accgtgtctt agccagttcc ttacagcttg ccctgatggg
    12661 agatagagaa tgggtatcct ccaacaaaaa aataaatttt ca
//
