LOCUS       sequence-445031-       12741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-1_Xt Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-Tc1-1_Xt-10 kb
FEATURES             Location/Qualifiers
     source          1..12741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1383..1406)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1406..1415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1430..2608)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1439..2377
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1462..1482)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    2654..2878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2867..2884
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2906..3619,3620..3622,3623..3625))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2909..3619,3620..3622,3623..3625))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2951..2972)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3300..3319
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3559..3580
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3626..3682)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3689..4288)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3769..3789)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      4285..4294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4308..4807)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4356..4375)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4749..4772
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(5273..5290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5273..5289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5299..5317)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      5352..5381
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      5403..5990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5891..5910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6002..6029)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(6053..6244)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(6245..7036,7037..7105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAELGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6868..6887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7106..7208)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(7209..7240)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     7292..7309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7321..7337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7321..7337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7631..8025
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     polyA_signal    12500..12581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12537..12556)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcctccgcca tcttatcttt ctgcagttca ttgtacaggc cttcctgagg gttcttcctt
       61 ctcggctttc cccccatctc agggtcccgg ccacgtctct tgtccaaaac atcgtactcc
      121 tctcttcgtc ctagattgag ctcgttatag agctggttct ggccctgctg gtacgcgggg
      181 gcgtctgcgc tcctgctgaa cttcactctg gagcgatagg ctgcgaagtc gcgtggtggg
      241 gcatagggct ggtaatgctt gcgggtgggc ccggggcggc ggggagtcat gttcatgtag
      301 tcactgtgca ggagcctgct cctcttactc ctcacccaga aaataataaa ggccactgtt
      361 actagcaagc tatagcaagc caggactccc ccaaccacca ccagcaccca aaaatcacag
      421 gcgaagtcca gccccctcgt gtgcactgcg ccccccgccg ctggccggca cgcctctggg
      481 cgcagggaca ggggctgcga cgcgatggtg ggcgccggtg ttggtggtcg cggcgctggc
      541 gtcgtggtca gcactgtcag ttgcgtgcct ccaccgaaaa cagcggctga actatgccaa
      601 atcatacagt aataatcggc ctcgtcctcg gacctgagac ctgagatgag cagcacaccc
      661 gcattggcgc ttgcgtcttt agatccacta aaccttgatg gaactccaga tccctgctgc
      721 ttatctgagt cgctcttgta attgagaagg tattggggag gagaacctgg tttttgctga
      781 taccagtaga tcctgtaagg ccctacattg attcctgacc tcagtgtaca cgtcaaagaa
      841 gcggaggcac cggggcttgc actgagggag gatgattgcg tgagaacagg ttggctaccc
      901 ccccctccgg acccgccccc accggaacct ccaccgcctg agcccaggat cccactggac
      961 acggtgacgg ttgtaccttg tccccaaacg tccataccat agtagtaagt catcataccc
     1021 cgcgcacaat agtaaacagc tgtgtcttcg ggagttacac tattaagctg aagagagaac
     1081 tggtttttgg aagtatctgg atttatagac atcctgcttt tcacacttac agcataatca
     1141 ttataccatt tactgcggta gtatgttcta ccgagccatt ccaaccctcg actgggggac
     1201 tgcctgatcc aattccatgt cgcggagtta ctactcactg aatcacctga tatggcacag
     1261 gtaagactaa gagtctggga aggagtgacc agaccggggc cagattgttg gagttgcact
     1321 tgcggcctgg cggcgtggag cagcaaggcc agcggcagga gcaaggcggt cactggtaag
     1381 gccttatcgt catcgtcttt gtaatccatg gtggcgaccg gtggatccct cacgacacct
     1441 gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa
     1501 actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag
     1561 tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag
     1621 acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc cggtactcag
     1681 tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact
     1741 cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg
     1801 gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg
     1861 gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcga gcttgagact
     1921 acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct
     1981 gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat
     2041 cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa
     2101 aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga
     2161 ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg
     2221 ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa
     2281 gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa
     2341 agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg gcaaacccgt
     2401 tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg
     2461 gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct
     2521 aggcaccggg tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
     2581 gctctgccca ctgacgggca ccggagcctc acgttaatta accaaggtac ccattggcgg
     2641 ccgccctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     2701 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     2761 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     2821 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     2881 gaggctgatc agcgagctta attaattact tgtacagctc gtccatgccg agagtgatcc
     2941 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     3001 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     3061 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     3121 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     3181 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     3241 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     3301 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     3361 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     3421 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     3481 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     3541 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     3601 acagctcctc gcccttgctc accattggcc cgggattctc ttcgacatcc cctgcttgtt
     3661 tcaacaggga gaagttagtg gcatcgatgg caccgggctt gcgggtcatg caccaggtgc
     3721 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
     3781 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
     3841 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
     3901 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
     3961 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
     4021 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
     4081 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
     4141 gctcggtgac ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt
     4201 cggcgaacgc ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc
     4261 gcaccgtggg cttgtactcg gtccccatgg tggcgttggc tgcaggtcga aaggcccgga
     4321 gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg
     4381 cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg
     4441 gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg
     4501 ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta
     4561 gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg
     4621 aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc
     4681 ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc
     4741 caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc
     4801 cctacccggt agaattgcta gcaccggtga tgtctagctt ttgaggtgct tttggtctac
     4861 ttctctgtgt caggtagctc ctatttaagt gatttcttga ttgaaacagg tgtggcagta
     4921 atcaggcctg ggggtgacta cagaaattga tattgaaatt gaaaattgaa atgtgataaa
     4981 ccacagttaa gttatttttt aacaaggggg gcaatcactt tttcacacag ggccatgtag
     5041 atttggagtt ttttttctcc cttaataacg taaaccttca tttaaaaact gcattttgtg
     5101 ttcaattatg ttatctttga ctaatagtta acggtttttg atgagcagaa acatttaagt
     5161 gtgacaaaca tgcaaaagaa taagaaatca ggaagggggc aaatagtttt tcacaccact
     5221 gtaaagctta gagacgcaag acactgcgga tcgagacgca ttgcgtggtt cgactggccg
     5281 tcgttttaca acgaaagggc ctcgtgatac gcctattgac tccagcgtaa ctggactgca
     5341 atcaactcac tggctcacct tcacgggtgg gcctttcttc ggtagaaaat caaaggatct
     5401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgag gtaactggct
     5521 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5581 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5641 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5701 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5761 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5821 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5881 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5941 ttgagcatcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6001 acgcagaaag gcccacccga aggtgagcca ggtgattaca tttgggccct catcagaggt
     6061 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     6121 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     6181 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     6241 tcacttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6301 ccatagttgc ctgactcccc gtcgtgtaga taactacgat gcgggagggc ttaccatctg
     6361 gccccagttc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     6421 taaaccagcc agccgggagg gccgagcgca gaagtgatcc tgcaacttta tccgcctcca
     6481 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6541 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6601 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6661 aagcggttag ctccttcggt cctccgatcg ttgccagaag taagttggcc gcagtgttat
     6721 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtgagatgct
     6781 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6841 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6901 tgctcatcat tggaaaacgt tcttcggggc gtaaactctc aaggatctta ccgctgttga
     6961 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7021 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7081 cgacacggaa atgttgaata ctcattttag cttccttagc tcctgaaaat ctcgataact
     7141 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     7201 gccgatcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt
     7261 atgatttaaa tggtcagtaa cgaccgagcg cagcgagtca gtgagcgagg aagcggcaca
     7321 caggaaacag ctatgaccgt ctcggttggc agtgactccg tctctgggcc ctacagtggt
     7381 gtgaaaaact atttgccccc ttcctgattt cttattcttt tgcatgtttg tcacacaaaa
     7441 tgtttctgat catcaaacac atttaactat tagtcaaaga taacacaagt aaacacaaaa
     7501 tgcagttttt aaatgagggt ttttattatt tagggagaaa aaaaatccaa acctacatgg
     7561 ccctgtgtga aaaagtaatt gccccctgaa cctcgagggc ccatgaggac agctaaaaca
     7621 ataagtaatg taaaatacag catagcaaaa ctttaacctc caaatcaagc ctctacttga
     7681 atccttttct gagggatgaa taaggcatag gcatcagggg ctgttgccaa tgtgcattag
     7741 ctgtttgcag cctcaccttc tttcatggag tttaagatat agtgtatttt cccaaggttt
     7801 gaactagctc ttcatttctt tatgttttaa atgcactgac ctcccacatt ccctttttag
     7861 taaaatattc agaaataatt taaatacatc attgcaatga aaataaatgt tttttattag
     7921 gcagaatcca gatgctcaag gcccttcata atatccccca gtttagtagt tggacttagg
     7981 gaacaaagga acctttaata gaaattggac agcaagaaag cgagcttagt gatacttgtg
     8041 ggccagggca ttagccacac cagccaccac tttctgatag gcagcctgca ctggtggggt
     8101 gaattctttg ccaaagtgat gggccagcac acagaccagc acgttgccca ggagctgtgg
     8161 gaggaagata agaggtatga acatgattag caaaagggcc tagcttggac tcagaataat
     8221 ccagccttat cccaaccata aaataaaagc agaatggtag ctggattgta gctgctatta
     8281 gcaatatgaa acctcttaca tcagttacaa tttatatgca gaaatattta tatgcagaaa
     8341 tattgctatt gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagagg
     8401 catgatacat tgtatcatta ttgccctgaa agaaagagat tagggaaagt attagaaata
     8461 agataaacaa aaaagtatat taaaagaaga aagcattttt taaaattaca aatgcaaaat
     8521 taccctgatt tggtcaatat gtgtacacat attaaaacat tacactttaa cccataaata
     8581 tgtataatga ttatgtatca attaaaaata aaagaaaata aagtagggag attatgaata
     8641 tgcaaataag cacacatata ttccaaatag taatgtacta ggcagactgt gtaaagtttt
     8701 tttttaagtt acttaatgta tctcagagat atttcctttt gttatacaca atgttaaggc
     8761 attaagtata atagtaaaaa ttgcggagaa gaaaaaaaaa gaaagcaaga attaaacaaa
     8821 agaaaacaat tgttatgaac agcaaataaa agaaactaaa acgatcctga gacttccaca
     8881 ctgatgcaat cattcgtctg tttcccattc taaactgtac cctgttactt ctccccttcc
     8941 tatgacatga acttaaccat agaaaagaag gggaaagaaa acatcaaggg tcccatagac
     9001 tcaccctgaa gttctcagga tccacgtgca gcttgtcaca gtgcagctca ctcagtgtgg
     9061 caaaggtgcc cttgaggttg tccaggtgag ccaggccatc actaaaggca ccgagcactt
     9121 tcttgccatg agccttcacc ttagggttgc ccataacagc atcaggagtg gacagatccc
     9181 caaaggactc aaagaacctc tgggtccaag ggtagaccac cagcagccta agggtgggaa
     9241 aatagaccaa taggcagaga gagtcagtgc ctatcagaaa cccaagagtc ttctctgtct
     9301 ccacatgccc agtttctatt ggtctcctta aacctgtctt gtaaccttga taccaacctg
     9361 cccagggcct caccaccaac ttcatccacg ttcaccttgc cccacagggc agtaacggca
     9421 gacttctcct caggagtcag gtgcaccatg gtgtctgttt gaggttgcta gtgaacacag
     9481 ttgtgtcaga agcaaatgta agcaatagat ggctctgccc tgacttttat gcccagccct
     9541 ggctcctgcc ctccctgctc ctgggagtag attggccaac cctagggtgt ggctccacag
     9601 ggtgaggtct aagtgatgac agccgtacct gtccttggct cttctggcac tggcttagga
     9661 gttggacttc aaaccctcag ccctccctct aagatatatc tcttggcccc ataccatcag
     9721 tacaaattgc tactaaaaac atcctccttt gcaagtgtat ttacgcttct ttgagaaaca
     9781 tcttcttcgt tagtggcctg cccctcattc ccactttaat atccagaatc actataagaa
     9841 gaatataata agaggaataa ctcttattat aggtaaggga aaattaagag gcatacgtga
     9901 tgggatgagt aagagaggag agggaaggat taatggatga taaaatctac tactatttgt
     9961 tgagaccttt tatagtctaa tcaattttgc tattgttttc catcctcacg ctaactccat
    10021 aaaaaaacac tattattatc tttattttgc catgacaaga ctgagctcag aagagtcaag
    10081 catttgccta aggtcggaca tgtcagaggc agtgccagac ctatgtgaga ctctgcagct
    10141 actgctcatg ggccctgtgc tgcactgatg aggaggatca gatggatggg gcaatgaagc
    10201 aaaggaatca ttctgtggat aaaggagaca gccatgaaga agtctatgac tgtaaatttg
    10261 ggagcaggag tctctaagga cttggatttc aaggaatttt gactcagcaa acacaagacc
    10321 ctcacggtga ctttgcgagc tggtgtgcca gatgtgtcta tcagaggttc cagggagggt
    10381 ggggtggggt cagggctggc caccagctat cagggcccag atgggttata ggctggcagg
    10441 ctcagatagg tggttaggtc aggttggtgg tgctgggtgg agtccatgac tcccaggagc
    10501 caggagagat agaccatgag tagagggcag acatgggaaa ggtgggggag gcacagcata
    10561 gcagcatttt tcattctact actacatggg actgctcccc tataccccca gctaggggca
    10621 agtgccttga ctcctatgtt ttcaggatca tcatctataa agtaagagta ataattgtgt
    10681 ctatctcata gggttattat gaggatcaaa ggagatgcac actctctgga ccagtggcct
    10741 aacagttcag gacagagcta tgggcttcct atgtatgggt cagtggtctc aatgtagcag
    10801 gcaagttcca gaagatagca tcaaccactg ttagagatat actgccagtc tcagagcctg
    10861 atgttaattt agcaatgggc tgggaccctc ctccagtaga accttctaac cagctgctgc
    10921 agtcaaagtc gaatgcagct ggttagactt tttttaatga aagcttgtac cagtggggcc
    10981 tctaagacta agtcactctg tctcaccgtg tcttagccag ttccttacag cttgccctga
    11041 tgggagatag agaatgggta tcctccaaca aaaaaataaa ttttcatttc tcaaggtcca
    11101 acttatgttt tcttaatttt taaaaaaatc ttgaccattc tccactctct aaaataatcc
    11161 acagtgagag aaacattctt ttcccccatc ccataaatac ctctattaaa tatggaaaat
    11221 ctgggcatgg tgtctcacac ctgtaatccc agcactttgg gaggctgagg tgggtggact
    11281 gcttggagct caggagttca agaccatctt ggacaacatg gtgataccct gcctctacaa
    11341 aaagtacaaa aattagcctg gcatggtggt gtgcacctgt aatcccagct attagggtgg
    11401 ctgaggcagg agaattgctt gaacccggga ggcggaggtt gcagtgagct gagatcgtgc
    11461 cactgcactc cagcctgggg gacagagcac attataatta actgttattt tttacttgga
    11521 ctcttgtggg gaataagata catgttttat tcttatttat gattcaagca ctgaaaatag
    11581 tgtttagcat ccagcaggtg cttcaaaacc atttgctgaa tgattactat actttttaca
    11641 agctcagctc cctctatccc ttccagcatc ctcatctctg attaaataag cttcagtttt
    11701 tccttagttc ctgttacatt tctgtgtgtc tccattagtg acctcccata gtccaagcat
    11761 gagcagttct ggccaggccc ctgtcggggt cagtgcccca cccccgcctt ctggttctgt
    11821 gtaaccttct aagcaaacct tctggctcaa gcacagcaat gctgagtcat gatgagtcat
    11881 gctgaggctt agggtgtgtg cccagatgtt ctcagcctag agtgatgact cctatctggg
    11941 tccccagcag gatgcttaca gggcagatgg caaaaaaaag gagaagctga ccacctgact
    12001 aaaactccac ctcaaacggc atcataaaga aaatggatgc ctgagacaga atgtgacata
    12061 ttctagaata tattatttcc tgaatatata tatatatata tatacatata cgtatatata
    12121 tatatatata tatatttgtt gttatcaatt gccatagaat gattagttat tgtgaatcaa
    12181 atatttatct tgcaggtggc ctctatacct agaagcggca gaatcaggct ttattaatac
    12241 atgtgtatag atttttagga tctatacaca tgtattaata tgaaacaagg atatggaaga
    12301 ggaaggcatg aaaacaggaa aagaaaacaa accttgtttg ccattttaag gcacccctgg
    12361 acagctaggt ggcaaaagga agcttccctt tggtctcccc ctcttaagta ccacatttgt
    12421 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    12481 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    12541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12601 caaactcaat cgatgcgagg gggcagggcc tgcatgtgaa gggcgtcgta ggtgtccttg
    12661 gtggctgtac tgagaccctg gtaaaggcca tcgtgcccct tgcccctccg gcgctcgcct
    12721 ttcatcccaa tctcactgta g
//
