LOCUS       sequence-445051-        5572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  promoter activity study.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Nguyen VT, Benveniste EN
  TITLE     Critical role of tumor necrosis factor-alpha and NF-kappa B in 
            interferon-gamma -induced CD40 expression in microglia/macrophages.
  JOURNAL   J Biol Chem. 2002 Apr 19;277(16):13796-803. Epub 2002 Feb 5.
  PUBMED    11830590
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-04-19"; volume: "277(16)"; pages: "13796-803. 
            Epub 2002 Feb 5"
COMMENT     Sequence Label: human CD40 promoter
FEATURES             Location/Qualifiers
     source          1..5572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    111..159
                     /note="synthetic polyadenylation signal"
     misc_feature    173..264
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     213..232
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1107..1116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1113..2765
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1179..1197)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2806..2927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2817..2836)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2871..2890
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3175..3192)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3346..3934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3426..3445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4105..4896,4897..4965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4728..4747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4966..5070)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5097..5552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5184..5203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5394..5415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
       61 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
      121 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
      181 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
      241 gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg tgctagcccc
      301 cgcgcgcgaa tccgatctgc ccgcctcggc ctcctaaagt gctggattac aggcgtgagc
      361 caccgcgccc ggccccactc ttaataaatg cctgtctcca ggtgctgggt gggaggtggg
      421 atggaatgga atgaggtgag gacgcatgga tgcatggatg aatggatggg aagttgagac
      481 gacgcgccca cacaagggaa tttcctttga aagagagcga aattctgagt tgggaaactc
      541 ttccttgaaa cgcctcccca taccccagct gtggccttcc cgttttctgc gtggtggtgt
      601 ggggggaact tcctcaggcc tctccgcagt ggagcctctt tcggttctgc caggatacct
      661 agaggcagcg gagagcgggg cagggagggg aaaaccgtga gggtccctgt ggcaggcccc
      721 agcacccatg ggatctctct ccggtcgcag gaagcaggct agctcctagc ccgcctcggc
      781 ttggcctttg tgggacctgg gggcaaagaa gaagagctgt ctctgggatc atgcctcctc
      841 ccgtacacag caagatgcgt ccctaaactc ccgggggaat tagacttgtg ggaatgttct
      901 ggggaaactc ctgcgcggtg aattgctggg ggctccgccc ccccgatagg tggaccgcga
      961 ttggtctttg aagaccccgc ccctttcctg ggcggggcca aggctggggc aggggagtca
     1021 gcagaggcct cgctcgggcg cccagtggtc ctgccgcctg gtctcacctc gcctctagag
     1081 cttggcattc cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag
     1141 gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta
     1201 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg
     1261 acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat
     1321 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta
     1381 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata
     1441 atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca
     1501 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta
     1561 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat
     1621 ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca
     1681 agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc
     1741 tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc
     1801 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt
     1861 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag
     1921 aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa
     1981 ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac
     2041 acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga
     2101 ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc
     2161 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg
     2221 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac
     2281 tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg
     2341 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg
     2401 aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg
     2461 ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg
     2521 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg
     2581 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga
     2641 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac
     2701 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg
     2761 tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     2821 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2881 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2941 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3001 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
     3061 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     3121 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     3181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4081 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4141 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4201 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4261 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4321 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4381 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4441 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4501 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4561 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4621 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4681 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4741 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4801 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4861 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4921 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4981 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5041 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     5101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5521 gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc tg
//
