LOCUS       sequence-445055-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of GFP using a self-replicating Venezuelan equine 
            encephalitis (VEE) virus RNA replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: SP6-VEE-GFP
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      68..656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     557..576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        678..696
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     678..695
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     RBS             2230..2238
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      8295..8304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8301..8303,8304..8306,8307..9020)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8301..8303,8304..8306,8307..9017)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8346..8367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8607..8626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8954..8975
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9105..9654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9249..9266)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9476..9495
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9657..10256
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9657..9676)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10153..10173
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        10453..10557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10558..10626,10627..11418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10776..10795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
       61 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      181 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
      241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      421 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      661 ccagcaacgc gagctcgatt taggtgacac tatagatggg cggcgcatga gagaagccca
      721 gaccaattac ctacccaaaa tggagaaagt tcacgttgac atcgaggaag acagcccatt
      781 cctcagagct ttgcagcgga gcttcccgca gtttgaggta gaagccaagc aggtcactga
      841 taatgaccat gctaatgcca gagcgttttc gcatctggct tcaaaactga tcgaaacgga
      901 ggtggaccca tccgacacga tccttgacat tggaagtgcg cccgcccgca gaatgtattc
      961 taagcacaag tatcattgta tctgtccgat gagatgtgcg gaagatccgg acagattgta
     1021 taagtatgca actaagctga agaaaaactg taaggaaata actgataagg aattggacaa
     1081 gaaaatgaag gagctggccg ccgtcatgag cgaccctgac ctggaaactg agactatgtg
     1141 cctccacgac gacgagtcgt gtcgctacga agggcaagtc gctgtttacc aggatgtata
     1201 cgcggttgac ggaccgacaa gtctctatca ccaagccaat aagggagtta gagtcgccta
     1261 ctggataggc tttgacacca ccccttttat gtttaagaac ttggctggag catatccatc
     1321 atactctacc aactgggccg acgaaaccgt gttaacggct cgtaacatag gcctatgcag
     1381 ctctgacgtt atggagcggt cacgtagagg gatgtccatt cttagaaaga agtatttgaa
     1441 accatccaac aatgttctat tctctgttgg ctcgaccatc taccacgaga agagggactt
     1501 actgaggagc tggcacctgc cgtctgtatt tcacttacgt ggcaagcaaa attacacatg
     1561 tcggtgtgag actatagtta gttgcgacgg gtacgtcgtt aaaagaatag ctatcagtcc
     1621 aggcctgtat gggaagcctt caggctatgc tgctacgatg caccgcgagg gattcttgtg
     1681 ctgcaaagtg acagacacat tgaacgggga gagggtctct tttcccgtgt gcacgtatgt
     1741 gccagctaca ttgtgtgacc aaatgactgg catactggca acagatgtca gtgcggacga
     1801 cgcgcaaaaa ctgctggttg ggctcaacca gcgtatagtc gtcaacggtc gcacccagag
     1861 aaacaccaat accatgaaaa attacctttt gcccgtagtg gcccaggcat ttgctaggtg
     1921 ggcaaaggaa tataaggaag atcaagaaga tgaaaggcca ctaggactac gagatagaca
     1981 gttagtcatg gggtgttgtt gggcttttag aaggcacaag ataacatcta tttataagcg
     2041 cccggatacc caaaccatca tcaaagtgaa cagcgatttc cactcattcg tgctgcccag
     2101 gataggcagt aacacattgg agatcgggct gagaacaaga atcaggaaaa tgttagagga
     2161 gcacaaggag ccgtcacctc tcattaccgc cgaggacgta caagaagcta agtgcgcagc
     2221 cgatgaggct aaggaggtgc gtgaagccga ggagttgcgc gcagctctac cacctttggc
     2281 agctgatgtt gaggagccca ctctggaggc agacgtcgac ttgatgttac aagaggctgg
     2341 ggccggctca gtggagacac ctcgtggctt gataaaggtt accagctacg atggcgagga
     2401 caagatcggc tcttacgctg tgctttctcc gcaggctgta ctcaagagtg aaaaattatc
     2461 ttgcatccac cctctcgctg aacaagtcat agtgataaca cactctggcc gaaaagggcg
     2521 ttatgccgtg gaaccatacc atggtaaagt agtggtgcca gagggacatg caatacccgt
     2581 ccaggacttt caagctctga gtgaaagtgc caccattgtg tacaacgaac gtgagttcgt
     2641 aaacaggtac ctgcaccata ttgccacaca tggaggagcg ctgaacactg atgaagaata
     2701 ttacaaaact gtcaagccca gcgagcacga cggcgaatac ctgtacgaca tcgacaggaa
     2761 acagtgcgtc aagaaagaac tagtcactgg gctagggctc acaggcgagc tggtggatcc
     2821 tcccttccat gaattcgcct acgagagtct gagaacacga ccagccgctc cttaccaagt
     2881 accaaccata ggggtgtatg gcgtgccagg atcaggcaag tctggcatca ttaaaagcgc
     2941 agtcaccaaa aaagatctag tggtgagcgc caagaaagaa aactgtgcag aaattataag
     3001 ggacgtcaag aaaatgaaag ggctggacgt caatgccaga actgtggact cagtgctctt
     3061 gaatggatgc aaacaccccg tagagaccct gtatattgac gaagcttttg cttgtcatgc
     3121 aggtactctc agagcgctca tagccattat aagacctaaa aaggcagtgc tctgcgggga
     3181 tcccaaacag tgcggttttt ttaacatgat gtgcctgaaa gtgcatttta accacgagat
     3241 ttgcacacaa gtcttccaca aaagcatctc tcgccgttgc actaaatctg tgacttcggt
     3301 cgtctcaacc ttgttttacg acaaaaaaat gagaacgacg aatccgaaag agactaagat
     3361 tgtgattgac actaccggca gtaccaaacc taagcaggac gatctcattc tcacttgttt
     3421 cagagggtgg gtgaagcagt tgcaaataga ttacaaaggc aacgaaataa tgacggcagc
     3481 tgcctctcaa gggctgaccc gtaaaggtgt gtatgccgtt cggtacaagg tgaatgaaaa
     3541 tcctctgtac gcacccacct cagaacatgt gaacgtccta ctgacccgca cggaggaccg
     3601 catcgtgtgg aaaacactag ccggcgaccc atggataaaa acactgactg ccaagtaccc
     3661 tgggaatttc actgccacga tagaggagtg gcaagcagag catgatgcca tcatgaggca
     3721 catcttggag agaccggacc ctaccgacgt cttccagaat aaggcaaacg tgtgttgggc
     3781 caaggcttta gtgccggtgc tgaagaccgc tggcatagac atgaccactg aacaatggaa
     3841 cactgtggat tattttgaaa cggacaaagc tcactcagca gagatagtat tgaaccaact
     3901 atgcgtgagg ttctttggac tcgatctgga ctccggtcta ttttctgcac ccactgttcc
     3961 gttatccatt aggaataatc actgggataa ctccccgtcg cctaacatgt acgggctgaa
     4021 taaagaagtg gtccgtcagc tctctcgcag gtacccacaa ctgcctcggg cagttgccac
     4081 tggaagagtc tatgacatga acactggtac actgcgcaat tatgatccgc gcataaacct
     4141 agtacctgta aacagaagac tgcctcatgc tttagtcctc caccataatg aacacccaca
     4201 gagtgacttt tcttcattcg tcagcaaatt gaagggcaga actgtcctgg tggtcgggga
     4261 aaagttgtcc gtcccaggca aaatggttga ctggttgtca gaccggcctg aggctacctt
     4321 cagagctcgg ctggatttag gcatcccagg tgatgtgccc aaatatgaca taatatttgt
     4381 taatgtgagg accccatata aataccatca ctatcagcag tgtgaagacc atgccattaa
     4441 gcttagcatg ttgaccaaga aagcttgtct gcatctgaat cccggcggaa cctgtgtcag
     4501 cataggttat ggttacgctg acagggccag cgaaagcatc attggtgcta tagcgcggca
     4561 gttcaagttt tcccgggtat gcaaaccgaa atcctcactt gaagagacgg aagttctgtt
     4621 tgtattcatt gggtacgatc gcaaggcccg tacgcacaat tcttacaagc tttcatcaac
     4681 cttgaccaac atttatacag gttccagact ccacgaagcc ggatgtgcac cctcatatca
     4741 tgtggtgcga ggggatattg ccacggccac cgaaggagtg attataaatg ctgctaacag
     4801 caaaggacaa cctggcggag gggtgtgcgg agcgctgtat aagaaattcc cggaaagctt
     4861 cgatttacag ccgatcgaag taggaaaagc gcgactggtc aaaggtgcag ctaaacatat
     4921 cattcatgcc gtaggaccaa acttcaacaa agtttcggag gttgaaggtg acaaacagtt
     4981 ggcagaggct tatgagtcca tcgctaagat tgtcaacgat aacaattaca agtcagtagc
     5041 gattccactg ttgtccaccg gcatcttttc cgggaacaaa gatcgactaa cccaatcatt
     5101 gaaccatttg ctgacagctt tagacaccac tgatgcagat gtagccatat actgcaggga
     5161 caagaaatgg gaaatgactc tcaaggaagc agtggctagg agagaagcag tggaggagat
     5221 atgcatatcc gacgactctt cagtgacaga acctgatgca gagctggtga gggtgcatcc
     5281 gaagagttct ttggctggaa ggaagggcta cagcacaagc gatggcaaaa ctttctcata
     5341 tttggaaggg accaagtttc accaggcggc caaggatata gcagaaatta atgccatgtg
     5401 gcccgttgca acggaggcca atgagcaggt atgcatgtat atcctcggag aaagcatgag
     5461 cagtattagg tcgaaatgcc ccgtcgaaga gtcggaagcc tccacaccac ctagcacgct
     5521 gccttgcttg tgcatccatg ccatgactcc agaaagagta cagcgcctaa aagcctcacg
     5581 tccagaacaa attactgtgt gctcatcctt tccattgccg aagtatagaa tcactggtgt
     5641 gcagaagatc caatgctccc agcctatatt gttctcaccg aaagtgcctg cgtatattca
     5701 tccaaggaag tatctcgtgg aaacaccacc ggtagacgag actccggagc catcggcaga
     5761 gaaccaatcc acagagggga cacctgaaca accaccactt ataaccgagg atgagaccag
     5821 gactagaacg cctgagccga tcatcatcga agaggaagaa gaggatagca taagtttgct
     5881 gtcagatggc ccgacccacc aggtgctgca agtcgaggca gacattcacg ggccgccctc
     5941 tgtatctagc tcatcctggt ccattcctca tgcatccgac tttgatgtgg acagtttatc
     6001 catacttgac accctggagg gagctagcgt gaccagcggg gcaacgtcag ccgagactaa
     6061 ctcttacttc gcaaagagta tggagtttct ggcgcgaccg gtgcctgcgc ctcgaacagt
     6121 attcaggaac cctccacatc ccgctccgcg cacaagaaca ccgtcacttg cacccagcag
     6181 ggcctgctcg agaaccagcc tagtttccac cccgccaggc gtgaataggg tgatcactag
     6241 agaggagctc gaggcgctta ccccgtcacg cactcctagc aggtcggtct cgagaaccag
     6301 cctggtctcc aacccgccag gcgtaaatag ggtgattaca agagaggagt ttgaggcgtt
     6361 cgtagcacaa caacaatgac ggtttgatgc gggtgcatac atcttttcct ccgacaccgg
     6421 tcaagggcat ttacaacaaa aatcagtaag gcaaacggtg ctatccgaag tggtgttgga
     6481 gaggaccgaa ttggagattt cgtatgcccc gcgcctcgac caagaaaaag aagaattact
     6541 acgcaagaaa ttacagttaa atcccacacc tgctaacaga agcagatacc agtccaggaa
     6601 ggtggagaac atgaaagcca taacagctag acgtattctg caaggcctag ggcattattt
     6661 gaaggcagaa ggaaaagtgg agtgctaccg aaccctgcat cctgttcctt tgtattcatc
     6721 tagtgtgaac cgtgcctttt caagccccaa ggtcgcagtg gaagcctgta acgccatgtt
     6781 gaaagagaac tttccgactg tggcttctta ctgtattatt ccagagtacg atgcctattt
     6841 ggacatggtt gacggagctt catgctgctt agacactgcc agtttttgcc ctgcaaagct
     6901 gcgcagcttt ccaaagaaac actcctattt ggaacccaca atacgatcgg cagtgccttc
     6961 agcgatccag aacacgctcc agaacgtcct ggcagctgcc acaaaaagaa attgcaatgt
     7021 cacgcaaatg agagaattgc ccgtattgga ttcggcggcc tttaatgtgg aatgcttcaa
     7081 gaaatatgcg tgtaataatg aatattggga aacgtttaaa gaaaacccca tcaggcttac
     7141 tgaagaaaac gtggtaaatt acattaccaa attaaaagga ccaaaagctg ctgctctttt
     7201 tgcgaagaca cataatttga atatgttgca ggacatacca atggacaggt ttgtaatgga
     7261 cttaaagaga gacgtgaaag tgactccagg aacaaaacat actgaagaac ggcccaaggt
     7321 acaggtgatc caggctgccg atccgctagc aacagcgtat ctgtgcggaa tccaccgaga
     7381 gctggttagg agattaaatg cggtcctgct tccgaacatt catacactgt ttgatatgtc
     7441 ggctgaagac tttgacgcta ttatagccga gcacttccag cctggggatt gtgttctgga
     7501 aactgacatc gcgtcgtttg ataaaagtga ggacgacgcc atggctctga ccgcgttaat
     7561 gattctggaa gacttaggtg tggacgcaga gctgttgacg ctgattgagg cggctttcgg
     7621 cgaaatttca tcaatacatt tgcccactaa aactaaattt aaattcggag ccatgatgaa
     7681 atctggaatg ttcctcacac tgtttgtgaa cacagtcatt aacattgtaa tcgcaagcag
     7741 agtgttgaga gaacggctaa ccggatcacc atgtgcagca ttcattggag atgacaatat
     7801 cgtgaaagga gtcaaatcgg acaaattaat ggcagacagg tgcgccacct ggttgaatat
     7861 ggaagtcaag attatagatg ctgtggtggg cgagaaagcg ccttatttct gtggagggtt
     7921 tattttgtgt gactccgtga ccggcacagc gtgccgtgtg gcagaccccc taaaaaggct
     7981 gtttaagctt ggcaaacctc tggcagcaga cgatgaacat gatgatgaca ggagaagggc
     8041 attgcatgaa gagtcaacac gctggaaccg agtgggtatt ctttcagagc tgtgcaaggc
     8101 agtagaatca aggtatgaaa ccgtaggaac ttccatcata gttatggcca tgactactct
     8161 agctagcagt gttaaatcat tcagctacct gagaggggcc cctataactc tctacggcta
     8221 acctgaatgg actacgacat agtctagtcc gccaagtcta gcatatgggc gcgccctcag
     8281 catcgattca attcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     8341 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     8401 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     8461 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     8521 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     8581 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     8641 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     8701 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     8761 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     8821 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     8881 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     8941 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     9001 tggacgagct gtacaagtag tctagagtcg acccgggcgg ccgcaactaa cttaagctag
     9061 caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag
     9121 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     9181 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     9241 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     9301 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     9361 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     9421 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     9481 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     9541 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     9601 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatga
     9661 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     9721 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     9781 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     9841 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     9901 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     9961 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
    10021 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
    10081 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
    10141 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
    10201 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagaat
    10261 tggcaagctg cttacataga actcgcggcg attggcatgc cgccttaaaa tttttatttt
    10321 attttttctt ttcttttccg aatcggattt tgtttttaat atttcaaaaa aaaaaaaaaa
    10381 aaaaaaaaac gcgtcgaggg gaattaattc ttgaagacga aagggccagg tggcactttt
    10441 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    10501 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    10561 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    10621 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10681 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10741 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10801 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10861 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10921 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10981 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    11041 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    11101 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    11161 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    11221 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    11281 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    11341 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    11401 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    11461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11521 a
//
