LOCUS       sequence-445111-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription for Oocyte expression of hTRAAK(S118C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Deal PE, Lee H, Mondal A, Lolicato M, Mendonca PRF, Black H, Jang S,
            El-Hilali X, Bryant C, Isacoff EY, Renslo AR, Minor DL Jr
  TITLE     Development of covalent chemogenetic K(2P) channel activators.
  JOURNAL   Cell Chem Biol. 2024 Jul 18;31(7):1305-1323.e9. doi: 
            10.1016/j.chembiol.2024.06.006.
  PUBMED    39029456
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2024.06"; journalName: "Cell Chem Biol"; date: 
            "2024-07-18- 18"; volume: "31"; issue: "7"; pages: "1305-1323.e9"
COMMENT     Sequence Label: pGEMHE:hTRAAK(S118C)
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1204..1222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1260..1282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1382..1401
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1595..1617
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1609..1626
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1610..1626
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1633..1652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1633..1651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           1683..1725
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(3222..3239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3393..3981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3473..3492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     1261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     1321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     1381 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     1441 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     1501 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     1561 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     1621 gccagtgaat tgtaatacga ctcactatag ggcgaattaa ttcgagctcg gtacccagct
     1681 tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagat caattccccg
     1741 gggatccgaa ttctctagag caagcttatg actaccgctc cacaagaacc acccgctcgc
     1801 ccactccaag ctggctccgg tgctggtcct gctcctggac gtgctatgag atcaacaacg
     1861 ctcctcgctc tgctggccct cgtattgctg tacctcgtgt ctggtgctct ggtattccgt
     1921 gctctcgaac aaccacatga acaacaggct caacgtgaac tgggagaagt ccgtgaaaaa
     1981 ttcctccgtg cccatccgtg tgtttccgat caagagttgg gattgctcat caaggaagtc
     2041 gccgacgctt tgggaggcgg tgccgaccct gagacaaact ccacctcaaa ctcctctcac
     2101 tctgcctggg atttgggatg tgccttcttc ttctctggaa ccatcatcac cactatcggt
     2161 tacggtaacg tggcactccg taccgacgcc ggtagactgt tctgtatttt ctacgccctc
     2221 gttggtatcc ctctcttcgg aatcttgttg gctggagtcg gagatcgcct cggttcatca
     2281 ttgaggcatg gcatcggtca tattgaggcc attttcttga aatggcacgt gccacctgaa
     2341 ttggttcgtg tactgtccgc tatgctgttc ctcctcatcg gttgcttgct cttcgttctc
     2401 acccccactt tcgtattctg ctacatggag gactggtcta aactggaggc catctacttc
     2461 gtgatcgtga ccctcactac tgttggattc ggagactacg ttgctggagc cgatcctaga
     2521 caggattctc ctgcctacca acctttggtg tggttctgga ttctgttggg actggcatac
     2581 ttcgctagtg tgctcaccac cattggcaac tggttgagag tggtatcacg tcgtactcgt
     2641 gctgagatgg gaggactcac tgctcaagct gcttcatgga ccggaactgt gactgctaga
     2701 gtcacccaac gtgctggacc ggctgctcca ccacctgaaa aagaacaacc cctgctgcct
     2761 cctccgcctt gtcccgccca accgctggga cgtcctcgtt ctccttcacc tcccgaaaag
     2821 gcacaaccac cttcaccccc tactgcttcc gctttggact acccttctga aaacctggct
     2881 ttcatcgacg aatcttcgga cactcaatcc gagcgtggat gtccattgcc tagggctcct
     2941 aggggccgtc gtagacccaa ccctccccgt aaacctgtta ggcctcgtgg acctggtaga
     3001 ccgagagaca aaggagtccc tgtttgactc gagtcgacgg atccgcggcc cgcgcatgcc
     3061 catggtctct agcttgatct ggttaccact aaaccagcct caagaacacc cgaatggagt
     3121 ctctaagcta cataatacca actcctgtcg tgccagctgc attaatgaat cggccaacgc
     3181 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3241 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3961 cgcagaaaaa aaggatctca a
//
