LOCUS       sequence-445159-        4652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-nsp13
FEATURES             Location/Qualifiers
     source          1..4652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3127..3226)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3243..3262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3244..3262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3267..3283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3267..3283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3714..4505
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4598..4652,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccgctgttg gggcttgtgt tctttgcaat
     1321 tcacagactt cattaagatg tggtgcttgc atacgtagac cattcttatg ttgtaaatgc
     1381 tgttacgacc atgtcatatc aacatcacat aaattagtct tgtctgttaa tccgtatgtt
     1441 tgcaatgctc caggttgtga tgtcacagat gtgactcaac tttacttagg aggtatgagc
     1501 tattattgta aatcacataa accacccatt agttttccat tgtgtgctaa tggacaagtt
     1561 tttggtttat ataaaaatac atgtgttggt agcgataatg ttactgactt taatgcaatt
     1621 gcaacatgtg actggacaaa tgctggtgat tacattttag ctaacacctg tactgaaaga
     1681 ctcaagcttt ttgcagcaga aacgctcaaa gctactgagg agacatttaa actgtcttat
     1741 ggtattgcta ctgtacgtga agtgctgtct gacagagaat tacatctttc atgggaagtt
     1801 ggtaaaccta gaccaccact taaccgaaat tatgtcttta ctggttatcg tgtaactaaa
     1861 aacagtaaag tacaaatagg agagtacacc tttgaaaaag gtgactatgg tgatgctgtt
     1921 gtttaccgag gtacaacaac ttacaaatta aatgttggtg attattttgt gctgacatca
     1981 catacagtaa tgccattaag tgcacctaca ctagtgccac aagagcacta tgttagaatt
     2041 actggcttat acccaacact caatatctca gatgagtttt ctagcaatgt tgcaaattat
     2101 caaaaggttg gtatgcaaaa gtattctaca ctccagggac cacctggtac tggtaagagt
     2161 cattttgcta ttggcctagc tctctactac ccttctgctc gcatagtgta tacagcttgc
     2221 tctcatgccg ctgttgatgc actatgtgag aaggcattaa aatatttgcc tatagataaa
     2281 tgtagtagaa ttatacctgc acgtgctcgt gtagagtgtt ttgataaatt caaagtgaat
     2341 tcaacattag aacagtatgt cttttgtact gtaaatgcat tgcctgagac gacagcagat
     2401 atagttgtct ttgatgaaat ttcaatggcc acaaattatg atttgagtgt tgtcaatgcc
     2461 agattacgtg ctaagcacta tgtgtacatt ggcgaccctg ctcaattacc tgcaccacgc
     2521 acattgctaa ctaagggcac actagaacca gaatatttca attcagtgtg tagacttatg
     2581 aaaactatag gtccagacat gttcctcgga acttgtcggc gttgtcctgc tgaaattgtt
     2641 gacactgtga gtgctttggt ttatgataat aagcttaaag cacataaaga caaatcagct
     2701 caatgcttta aaatgtttta taagggtgtt atcacgcatg atgtttcatc tgcaattaac
     2761 aggccacaaa taggcgtggt aagagaattc cttacacgta accctgcttg gagaaaagct
     2821 gtctttattt caccttataa ttcacagaat gctgtagcct caaagatttt gggactacca
     2881 actcaaactg ttgattcatc acagggctca gaatatgact atgtcatatt cactcaaacc
     2941 actgaaacag ctcactcttg taatgtaaac agatttaatg ttgctattac cagagcaaaa
     3001 gtaggcatac tttgcataat gtctgataga gacctttatg acaagttgca atttacaagt
     3061 cttgaaattc cacgtaggaa tgtggcaact ttacaactta attaagacgc gatcgcggcc
     3121 tcatgggccc agctttcttg tacaaagttg gcattataaa aaataattgc tcatcaattt
     3181 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     3241 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     3301 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     3361 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     3421 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     3481 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3541 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     3601 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     3661 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     3721 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     3781 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     3841 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     3901 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     3961 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     4021 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     4081 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     4141 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     4201 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     4261 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     4321 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     4381 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     4441 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     4501 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     4561 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4621 taatctgctg cttgcaaaca aaaaaaccac cg
//
