LOCUS       sequence-445160-        4658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp13
FEATURES             Location/Qualifiers
     source          1..4658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3133..3232)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3249..3268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3250..3268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3273..3289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3273..3289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3720..4511
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4604..4658,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccagtgttg gagcttgcgt ggtctgctct
     1321 tctcaaacat cattacgttg tggcagttgc atcagaaagc ctcttctttg ctgcaagtgt
     1381 tgttatgatc atgttatggc gactgatcat aaatatgtct tgagtgtttc accatatgtg
     1441 tgtaatgcac caggatgtga tgtaaatgat gttaccaaat tgtatctagg tggtatgtca
     1501 tattattgtg aagaccataa gccacaatat tcattcaagt tggtaatgaa tggtctggtt
     1561 tttggtctat ataaacaatc ttgtacagga tctccgtaca tagacgattt taatcgtata
     1621 gctagttgta aatggaccga tgtggatgat tacatactag ctaatgaatg tacagagcgc
     1681 ttgaaattgt ttgctgcaga aacgcaaaag gcaaccgagg aagcctttaa gcagagttat
     1741 gcatcagcaa caatacaaga gattgttagt gagcgcgaat tgattctctc ttgggagatt
     1801 ggaaaagtta agccaccact taataaaaat tatgttttta ctggctacca ttttactaaa
     1861 aatggtaaga cagttttagg tgagtatgtt tttgataaga gtgagttgac taatggtgtg
     1921 tattatcgcg ccacaaccac ttataagcta tctgtaggag atgtttttgt tttaacctct
     1981 cattcagtag ctaatttaag tgctcctacg cttgttccgc aggagaatta tagtagtatt
     2041 agatttgcta gtgtttatag tgtgcttgag acgtttcaga acaatgttgt taattatcaa
     2101 cacattggta tgaaacgtta ctgcaccgtg caaggacctc ctggtacagg gaagtcacat
     2161 cttgctattg gtcttgctgt attctattgt acagcacgtg ttgtatacac agcggccagc
     2221 catgcagctg ttgacgcatt gtgtgaaaaa gcatataaat ttttgaatat aaatgattgc
     2281 actcgtattg ttccggccaa ggtcagggtg gagtgctatg ataagtttaa aattaatgac
     2341 accactcgta agtatgtgtt tactaccata aatgcattac ctgagatggt gactgatatt
     2401 gttgttgtag atgaagttag tatgcttacc aattatgagc tttctgttat taatgctcgt
     2461 attcgcgcta agcattatgt ttatattggt gatcctgctc aattgccagc accacgtgtg
     2521 ttattgagca agggtacact tgaacctaaa tattttaaca ctgttactaa gctcatgtgt
     2581 tgcttagggc cagacatttt tcttggtaca tgttatagat gtcctaagga aatcgttgat
     2641 acagtgtccg ccttggttta tgaaaataag cttaaggcta agaatgagag tagttcattg
     2701 tgttttaagg tctattataa gggcgttaca acacatgaaa gttctagtgc tgtaaatatg
     2761 cagcagattt atttgattaa taagtttttg aaggctaacc ctttgtggca taaagctgtt
     2821 tttattagcc catataatag tcagaacttt gcagctaagc gtgttttggg tttacaaacc
     2881 caaaccgtgg attctgctca aggttctgaa tatgattatg ttatatattc acagactgca
     2941 gaaacagcgc attctgtaaa tgttaatcgc ttcaatgttg ctattactcg agccaagaaa
     3001 ggtattcttt gtgttatgag taatatgcag ttgtttgaag cattacagtt tactacattg
     3061 accttagata aagtgccaca ggccgtcgaa actaaagttc aacttaatta agacgcgatc
     3121 gcggcctcat gggcccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat
     3181 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     3241 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     3301 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
     3361 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     3421 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     3481 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     3541 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     3601 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     3661 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     3721 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     3781 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     3841 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     3901 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     3961 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     4021 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     4081 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     4141 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     4201 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     4261 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     4321 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     4381 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     4441 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     4501 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     4561 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4621 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccg
//
