LOCUS       sequence-445167-        4337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp4
FEATURES             Location/Qualifiers
     source          1..4337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2812..2911)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2928..2947)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2929..2947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2952..2968)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2952..2968)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3399..4190
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4283..4337,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccgcagttt ttagttattt tgtttatgtg
     1321 tgttttgtgt tgagtttggt ctgttttatt ggactgtggt gcttaatgcc cacttacaca
     1381 gtacacaaat cagattttca gcttcccgtt tatgccagtt ataaagtttt agataatggt
     1441 gttattagag atgttagcgt tgaagatgtt tgtttcgcta acaaatttga acaatttgat
     1501 caatggtatg agtctacatt tggtctaagt tattatagta acagtatggc ttgtcccatt
     1561 gttgttgctg taatagatca ggattttggc tctacagtgt ttaatgtccc taccaaagtg
     1621 ttacgatatg gttatcatgt gttgcacttt attacacatg cactttctgc tgatggagtg
     1681 cagtgttata cgccacatag tcaaatatcg tattctaatt tttatgctag tggctgtgtg
     1741 ctttcctctg cttgcactat gtttacaatg gccgatggta gtccacaacc ttattgttat
     1801 acagaggggc ttatgcaaaa tgcttctctg tatagttcat tggtacctca cgtgcggtat
     1861 aatcttgcta atgctaaagg ttttatccgt tttccagaag tgttgcgaga agggcttgta
     1921 cgtatcgtgc gtactcgttc tatgtcgtat tgcagagttg gattatgtga ggaagctgat
     1981 gagggtatat gctttaattt taatggttct tgggtgctta ataatgatta ttatagatca
     2041 ttgcctggga ccttttgtgg tagagatgtt tttgatttaa tttatcagct atttaaaggt
     2101 ttagcacagc ctgtggattt tttggcattg actgctagtt ccattgctgg tgctatactc
     2161 gctgtaattg ttgttttggt gttttattac ctaataaagc ttaaacgtgc ttttggtgat
     2221 tacaccagtg ttgtttttgt taacgtgatt gtgtggtgtg taaattttat gatgcttttt
     2281 gtgtttcaag tttaccccat actttcttgt gtatatgcta tttgttattt ttatgccacg
     2341 ctttatttcc cttcggagat aagtgtgata atgcacttac aatggctagt tatgtatggc
     2401 actattatgc ctttatggtt ttgtttgcta tatatagctg ttgttgtttc aaatcatgct
     2461 ttttgggtat tttcttactg cagaaagctt ggtacttctg ttcgtagtga tggtacattt
     2521 gaagaaatgg ctctcactac ttttatgatt acaaaagatt cttattgtaa gcttaagaat
     2581 tctttgtctg atgttgcttt taatagatat ttgagtttgt ataataaata taggtattac
     2641 agcggtaaaa tggatactgc tgcatatagg gaggctgctt gctctcagtt ggctaaagca
     2701 atggacacat ttaccaataa taatggtagt gatgtgcttt accaaccgcc tactgcttcc
     2761 gtctcaactt cattcttgca acttaattaa gacgcgatcg cggcctcatg ggcccagctt
     2821 tcttgtacaa agttggcatt ataaaaaata attgctcatc aatttgttgc aacgaacagg
     2881 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     2941 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     3001 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
     3061 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     3121 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     3181 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     3241 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     3301 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     3361 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     3421 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     3481 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     3541 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     3601 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     3661 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     3721 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     3781 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     3841 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     3901 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     3961 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     4021 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     4081 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     4141 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     4201 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     4261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4321 aaacaaaaaa accaccg
//
