LOCUS       sequence-445171-       10298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRICK co-localization control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Voigt F, Eglinger J, Chao JA
  TITLE     Detection of the First Round of Translation: The TRICK Assay.
  JOURNAL   Methods Mol Biol. 2018;1649:373-384. doi: 
            10.1007/978-1-4939-7213-5_25.
  PUBMED    29130211
REFERENCE   2  (bases 1 to 10298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1649:373-384. doi"; pages: " 
            10.1007/978-1-4939-7213-5_25"
COMMENT     Sequence Label: pSF4 TET CMV intron renilla 24xPP7 24xMS2
FEATURES             Location/Qualifiers
     source          1..10298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3806..3824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3842..3860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3878..3896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3914..3932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3950..3968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3986..4004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4085..4109
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4186..4318
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4301..4320
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4363..4381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4385..4394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4391..5326
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8096..8230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8174..8193
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8338..8371)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8424..8440)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(8456..8478)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    8889..9246
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9311..9780
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc tttactccct atcagtgata gagaacgtat gaagagttta
     3841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     3901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     3961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     4021 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     4081 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     4141 ataccaactt tccgtaccac ttcctaccct cgtaaaaccg gtcaggtaag tatcaaggtt
     4201 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     4261 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     4321 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
     4381 gctagccacc atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg
     4441 gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta
     4501 tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag
     4561 ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga
     4621 tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca
     4681 ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt
     4741 gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat
     4801 caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc
     4861 tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga
     4921 gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga
     4981 ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct
     5041 ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt
     5101 ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc
     5161 cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga
     5221 gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa
     5281 gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagtaagtcg acagatccta
     5341 cctgcaaagc accagaggat atggcctcgg tgcctcagca tcttcagatg tgctgtcact
     5401 gtccaatctt caagccagca gagcatatgg gctcgctggc tccactgtca ttacagtgga
     5461 cctgatcttc tccaatcaaa ggagcagagg atatggcctc gctccctccg actacatcag
     5521 gctggaagtg acatccatca ttcatcccag cagagcatat gggctcgctg ggaccacagc
     5581 atgcctcgaa gtgctgagag actatcatca aaccgacaga ggatatggcc tcgtcggctc
     5641 ctactccctc ctggtggaat acttctccac tcttcaaccc agcagagcat atgggctcgc
     5701 tgggtccatc agagatacaa gatctctcga cagatcctac ctgcaaagca ccagaggata
     5761 tggcctcggt gcctcagcat cttcagatgt gctgtcactg tccaatcttc aagccagcag
     5821 agcatatggg ctcgctggct ccactgtcat tacagtggac ctgatcttct ccaatcaaag
     5881 gagcagagga tatggcctcg ctccctccga ctacatcagg ctggaagtga catccatcat
     5941 tcatcccagc agagcatatg ggctcgctgg gaccacagca tgcctcgaag tgctgagaga
     6001 ctatcatcaa accgacagag gatatggcct cgtcggctcc tactccctcc tggtggaata
     6061 cttctccact cttcaaccca gcagagcata tgggctcgct gggtccatca gagatacaag
     6121 atctctcgac agatcctacc tgcaaagcac cagaggatat ggcctcggtg cctcagcatc
     6181 ttcagatgtg ctgtcactgt ccaatcttca agccagcaga gcatatgggc tcgctggctc
     6241 cactgtcatt acagtggacc tgatcttctc caatcaaagg agcagaggat atggcctcgc
     6301 tccctccgac tacatcaggc tggaagtgac atccatcatt catcccagca gagcatatgg
     6361 gctcgctggg accacagcat gcctcgaagt gctgagagac tatcatcaaa ccgacagagg
     6421 atatggcctc gtcggctcct actccctcct ggtggaatac ttctccactc ttcaacccag
     6481 cagagcatat gggctcgctg ggtccatcag agatacaaga tctctcgagc cgcggtgcgt
     6541 aagtctgtta acagaatgct gcatacgctg agtacttcga aatgtcatcc tctgtctgac
     6601 actgaacgta atccagacgc ggatcctacc tacaaacggc acgagcatca gccgtgccgc
     6661 ccagcatctt catcactgtc caatcttcaa gcgtgcacga tcacggcacg ctccactgtc
     6721 attacagtct tatccaatca aactccagca gcatcagcgc tggagctccg actacatcag
     6781 gacatccatc attcaacgct cgacgatcac gcgagcgtcc acagcatgcc tcagacacta
     6841 tcatcaaacg gctgcacgat cacggcagcc gctcctactg cctctacgtt atcgactctt
     6901 ctgcgacgag catcagccgt cgcaacatcg gacataccag atcctaccta caaacggcac
     6961 gagcatcagc cgtgccgccc agcatcttca tcactgtcca atcttcaagc gtgcacgatc
     7021 acggcacgct ccactgtcat tacagtctta tccaatcaaa ctccagcagc atcagcgctg
     7081 gagctccgac tacatcagga catccatcat tcaacgctcg acgatcacgc gagcgtccac
     7141 agcatgcctc agacactatc atcaaacggc tgcacgatca cggcagccgc tcctactgcc
     7201 tctacgttat cgactcttct gcgacgagca tcagccgtcg caacatcgga cataccagat
     7261 cctacctaca aacggcacga gcatcagccg tgccgcccag catcttcatc actgtccaat
     7321 cttcaagcgt gcacgatcac ggcacgctcc actgtcatta cagtcttatc caatcaaact
     7381 ccagcagcat cagcgctgga gctccgacta catcaggaca tccatcattc aacgctcgac
     7441 gatcacgcga gcgtccacag catgcctcag acactatcat caaacggctg cacgatcacg
     7501 gcagccgctc ctactgcctc tacgttatcg actcttctgc gacgagcatc agccgtcgca
     7561 acatcggaca taccagatcc tacctacaaa cggcacgagc atcagccgtg ccgcccagca
     7621 tcttcatcac tgtccaatct tcaagcgtgc acgatcacgg cacgctccac tgtcattaca
     7681 gtcttatcca atcaaactcc agcagcatca gcgctggagc tccgactaca tcaggacatc
     7741 catcattcaa cgctcgacga tcacgcgagc gtccacagca tgcctcagac actatcatca
     7801 aacggctgca cgatcacggc agccgctcct actgcctcta cgttatcgac tcttctgcga
     7861 cgagcatcag ccgtcgcaac atcggacata ccagatcttc aatcgatttc gcgcgggatc
     7921 cagaccacct cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt
     7981 ttcactaacc taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa
     8041 gtgataaaaa tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc
     8101 agacatgata agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa
     8161 atgccttatt tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa
     8221 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     8281 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctgaa
     8341 gttcctatac tatttgaaga ataggaactt cggaatagga acttcggatc tcatatggcc
     8401 atatgggggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg
     8461 aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag
     8521 ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga
     8581 tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct
     8641 gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc
     8701 attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag
     8761 cgatccgctc gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacaggct
     8821 agcccgggcc catattttca gacaaataca gaaacacagt cagacagaga caacacagaa
     8881 cgatgctgca gcagacaaga cgcgcggcgc ggcttcggtc ccaaaccgaa agcaaaaatt
     8941 cagacggagg cgggaactgt tttaggttct cgtctcctac cagaaccaca tatccctcct
     9001 ctaagggggg tgcaccaaag agtcctaaac gatcgggatt ttaggactca ggtcgggcca
     9061 caaaaacggc ccccgaagtc cctgggacgt ctcccagggt tgcggccggg tgttccgaac
     9121 tcgtcagttc caccacgggt ccgccagata cagagctagt tagctaacta gtaccgacgc
     9181 aggcgcataa aatcagtcat agacactaga caatcggaca gacacagata agttgctggc
     9241 cagcttacct cccggtggtg ggtcggtggt ccctgggcag gggtctcccg atcccggacg
     9301 agcccccaaa tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca
     9361 aggaacagcg agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta
     9421 cccgggcgac tcagtcaatc ggaggactgg cgcgccgagt gaggggttgt gggctctttt
     9481 attgagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
     9541 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
     9601 ctagaaactg ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
     9661 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     9721 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     9781 ctgttccatc tgttcctgac cttgatctga acttttctat tctcagttat gtatttttcc
     9841 atgccttgca aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca
     9901 catgtatatg tcaaaaataa aaatcaacta attgactagt aattaatatg actggcataa
     9961 tgggaaattg atcctgacag atgcaaactg gcttctcagc agcgcattta tgttgtcaac
    10021 tgaggaagga aacgttaatg acagaaactc taagtaattt ccacgtttat ctatttttat
    10081 ttatactagc tttggtaaca ggaatattgc agcattcatg cacattgaaa cccttatgaa
    10141 ataaaaacat ctgtgcattt aaaatggaat taacatttta aatgttaaaa aaagctggct
    10201 tagcttcccc ccgcccccta gggcatagaa caagtcaaat gctttatata tttgagtttg
    10261 ggatgtatta ggaaactcct aagagcaaag ctagcttg
//
