LOCUS       sequence-445183-        4403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-NL63-nsp14
FEATURES             Location/Qualifiers
     source          1..4403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2878..2977)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2994..3013)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2995..3013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3018..3034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3465..4256
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4349..4403,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctctagcc aggtttgtgg cttgtttaaa
     1321 aattgtacac gcactcctct taatttacca ccaactcatg cacacacttt cttgtcgttg
     1381 tcagatcagt ttaagactac aggtgattta gctgttcaaa taggttcaaa taacgtttgt
     1441 acttatgaac atgttatatc atttatgggt tttaggtttg atattagtat tcctggtagt
     1501 catagtttgt tttgtacacg tgactttgct attcgtaatg tgcgtggttg gttgggtatg
     1561 gatgttgaaa gtgctcatgt ttgtggcgat aacataggta ctaatgttcc tttacaggtt
     1621 ggtttttcaa atggtgttaa ttttgttgtg caaactgaag gttgtgtgtc taccaatttt
     1681 ggtgatgtta ttaaacctgt ttgtgcaaaa tctccaccag gtgaacaatt tagacacctt
     1741 attcctcttt tacgtaaagg acaaccttgg ttaattgttc gtagacgcat tgtgcaaatg
     1801 atatctgatt atttgtccaa tttgtctgac attcttgtct ttgttttgtg ggcaggtagt
     1861 ttggaattaa ctacaatgcg ttactttgta aaaatagggc caattaaata ttgttattgt
     1921 ggtaattctg ccacttgtta taattcagtt agtaatgaat attgttgttt taaacatgca
     1981 ttgggttgtg attatgttta caatccgtat gcttttgata tacaacagtg gggttatgtt
     2041 ggttccttga gccaaaacca ccacacattc tgtaacattc atagaaacga gcatgatgcc
     2101 tctggtgatg ctgttatgac acgttgtttg gcagtacatg attgttttgt caaaaatgtt
     2161 gattggactg taacgtaccc ctttattgca aatgagaaat ttatcaatgg ctgtgggcgt
     2221 aatgtccagg gacatgttgt tcgtgcagcc ttgaaattgt ataaacctag tgttattcat
     2281 gacattggta atcctaaagg tgtacgttgt gctgttactg atgccaaatg gtactgttat
     2341 gacaagcaac ctgttaatag taatgtcaag ttgttggatt atgattatgc aacccatggt
     2401 caacttgatg gtctttgttt attctggaat tgtaatgttg atatgtatcc agaattttca
     2461 attgtgtgtc gttttgacac acgtactcgt tctgttttta atttagaagg tgttaatggt
     2521 ggttctcttt atgttaacaa acatgcgttt catacaccag catatgataa acgtgctttt
     2581 gttaaattaa aacctatgcc ctttttttac tttgatgaca gtgattgtga tgttgtgcaa
     2641 gaacaagtta attatgtacc ccttcgcgct agtagttgtg ttactcgttg taatataggt
     2701 ggtgctgttt gttcaaaaca tgcaaatttg tatcaaaaat atgttgaggc atataataca
     2761 tttacacagg caggttttaa catttgggta ccacatagtt ttgatgttta taatttgtgg
     2821 caaattttta ttgaaactaa tttacaactt aattaagacg cgatcgcggc ctcatgggcc
     2881 cagctttctt gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg
     2941 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     3001 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     3061 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     3121 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     3181 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     3241 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3301 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3361 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3421 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     3481 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3541 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3601 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3661 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3721 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3781 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3841 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3901 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3961 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     4021 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     4081 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     4141 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     4201 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     4261 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4321 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4381 gcttgcaaac aaaaaaacca ccg
//
