LOCUS       sequence-445202-       10046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BRD4
FEATURES             Location/Qualifiers
     source          1..10046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     685..709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(813..815,816..818,819..1529)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(858..879)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1119..1138)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1466..1487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1545..1569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3840..3857
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(5741..5765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5773..5814
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5854..5873)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5898..5946
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6148..6576
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6235..6254)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6445..6466
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6585..6605)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6590..6919
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6770..6905
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6832..6851
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6967..7014
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7033..7431
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7589..7710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7626..7645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7680..7699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7759..7775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7759..7775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7772..7794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7783..7799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7807..7813,7814..7831,7832..7837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7852..7873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7990..8007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8161..8749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8241..8260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8920..9711,9712..9780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9543..9562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9781..9885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9960..9979)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      781 agaggatccc taccggtgat atcctcgaga ccatggtgag caagggcgag gagctgttca
      841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     1021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1261 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
     1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1501 tcactctcgg catggacgag ctgtacaagg gctcgagccc atcaacaagt ttgtacaaaa
     1561 aagcaggctt cgaaggagat agaaccaatt ctctaaggaa atacttaacc atggtcgact
     1621 ggatcctcat gtctgcggag agcggccctg ggacgagatt gagaaatctg ccagtaatgg
     1681 gggatggact agaaacttcc caaatgtcta caacacaggc ccaggcccaa ccccagccag
     1741 ccaacgcagc cagcaccaac cccccgcccc cagagacctc caaccctaac aagcccaaga
     1801 ggcagaccaa ccaactgcaa tacctgctca gagtggtgct caagacacta tggaaacacc
     1861 agtttgcatg gcctttccag cagcctgtgg atgccgtcaa gctgaacctc cctgattact
     1921 ataagatcat taaaacgcct atggatatgg gaacaataaa gaagcgcttg gaaaacaact
     1981 attactggaa tgctcaggaa tgtatccagg acttcaacac tatgtttaca aattgttaca
     2041 tctacaacaa gcctggagat gacatagtct taatggcaga agctctggaa aagctcttct
     2101 tgcaaaaaat aaatgagcta cccacagaag aaaccgagat catgatagtc caggcaaaag
     2161 gaagaggacg tgggaggaaa gaaacaggga cagcaaaacc tggcgtttcc acggtaccaa
     2221 acacaactca agcatcgact cctccgcaga cccagacccc tcagccgaat cctcctcctg
     2281 tgcaggccac gcctcacccc ttccctgccg tcaccccgga cctcatcgtc cagacccctg
     2341 tcatgacagt ggtgcctccc cagccactgc agacgccccc gccagtgccc ccccagccac
     2401 aacccccacc cgctccagct ccccagcccg tacagagcca cccacccatc atcgcggcca
     2461 ccccacagcc tgtgaagaca aagaagggag tgaagaggaa agcagacacc accaccccca
     2521 ccaccattga ccccattcac gagccaccct cgctgccccc ggagcccaag accaccaagc
     2581 tgggccagcg gcgggagagc agccggcctg tgaaacctcc aaagaaggac gtgcccgact
     2641 ctcagcagca cccagcacca gagaagagca gcaaggtctc ggagcagctc aagtgctgca
     2701 gcggcatcct caaggagatg tttgccaaga agcacgccgc ctacgcctgg cccttctaca
     2761 agcctgtgga cgtggaggca ctgggcctac acgactactg tgacatcatc aagcacccca
     2821 tggacatgag cacaatcaag tctaaactgg aggcccgtga gtaccgtgat gctcaggagt
     2881 ttggtgctga cgtccgattg atgttctcca actgctataa gtacaaccct cctgaccatg
     2941 aggtggtggc catggcccgc aagctccagg atgtgttcga aatgcgcttt gccaagatgc
     3001 cggacgagcc tgaggagcca gtggtggccg tgtcctcccc ggcagtgccc cctcccacca
     3061 aggttgtggc cccgccctca tccagcgaca gcagcagcga tagctcctcg gacagtgaca
     3121 gttcgactga tgactctgag gaggagcgag cccagcggct ggctgagctc caggagcagc
     3181 tcaaagccgt gcacgagcag cttgcagccc tctctcagcc ccagcagaac aaaccaaaga
     3241 aaaaggagaa agacaagaag gaaaagaaaa aagaaaagca caaaaggaaa gaggaagtgg
     3301 aagagaataa aaaaagcaaa gccaaggaac ctcctcctaa aaagacgaag aaaaataata
     3361 gcagcaacag caatgtgagc aagaaggagc cagcgcccat gaagagcaag ccccctccca
     3421 cgtatgagtc ggaggaagag gacaagtgca agcctatgtc ctatgaggag aagcggcagc
     3481 tcagcttgga catcaacaag ctccccggcg agaagctggg ccgcgtggtg cacatcatcc
     3541 agtcacggga gccctccctg aagaattcca accccgacga gattgaaatc gactttgaga
     3601 ccctgaagcc gtccacactg cgtgagctgg agcgctatgt cacctcctgt ttgcggaaga
     3661 aaaggaaacc tcaagctgag aaagttgatg tgattgccgg ctcctccaag atgaagggct
     3721 tctcgtcctc agagtcggag agctccagtg agtccagctc ctctgacagc gaagactccg
     3781 aaacagagat ggctccgaag tcaaaaaaga aggggcaccc cgggagggag cagaagaagc
     3841 accatcatca ccaccatcag cagatgcagc aggccccggc tcctgtgccc cagcagccgc
     3901 ccccgcctcc ccagcagccc ccaccgcctc cacctccgca gcagcaacag cagccgccac
     3961 ccccgcctcc cccaccctcc atgccgcagc aggcagcccc ggcgatgaag tcctcgcccc
     4021 cacccttcat tgccacccag gtgcccgtcc tggagcccca gctcccaggc agcgtctttg
     4081 accccatcgg ccacttcacc cagcccatcc tgcacctgcc gcagcctgag ctgccccctc
     4141 acctgcccca gccgcctgag cacagcactc caccccatct caaccagcac gcagtggtct
     4201 ctcctccagc tttgcacaac gcactacccc agcagccatc acggcccagc aaccgagccg
     4261 ctgccctgcc tcccaagccc gcccggcccc cagccgtgtc accagccttg acccaaacac
     4321 ccctgctccc acagcccccc atggcccaac ccccccaagt gctgctggag gatgaagagc
     4381 cacctgcccc acccctcacc tccatgcaga tgcagctgta cctgcagcag ctgcagaagg
     4441 tgcagccccc tacgccgcta ctcccttccg tgaaggtgca gtcccagccc ccaccccccc
     4501 tgccgccccc accccacccc tctgtgcagc agcagctgca gcagcagccg ccaccacccc
     4561 caccacccca gccccagcct ccaccccagc agcagcatca gccccctcca cggcccgtgc
     4621 acttgcagcc catgcagttt tccacccaca tccaacagcc cccgccaccc cagggccagc
     4681 agccccccca tccgccccca ggccagcagc cacccccgcc gcagcctgcc aagcctcagc
     4741 aagtcatcca gcaccaccat tcaccccggc accacaagtc ggacccctac tcaaccggtc
     4801 acctccgcga agccccctcc ccgcttatga tacattcccc ccagatgtca cagttccaga
     4861 gcctgaccca ccagtctcca ccccagcaaa acgtccagcc taagaaacag gagctgcgtg
     4921 ctgcctccgt ggtccagccc cagcccctcg tggtggtgaa ggaggagaag atccactcac
     4981 ccatcatccg cagcgagccc ttcagcccct cgctgcggcc ggagcccccc aagcacccgg
     5041 agagcatcaa ggcccccgtc cacctgcccc agcggccgga aatgaagcct gtggatgtcg
     5101 ggaggcctgt gatccggccc ccagagcaga acgcaccgcc accaggggcc cctgacaagg
     5161 acaaacagaa acaggagccg aagactccag ttgcgcccaa aaaggacctg aaaatcaaga
     5221 acatgggctc ctgggccagc ctagtgcaga agcatccgac caccccctcc tccacagcca
     5281 agtcatccag cgacagcttc gagcagttcc gccgcgccgc tcgggagaaa gaggagcgtg
     5341 agaaggccct gaaggctcag gccgagcacg ctgagaagga gaaggagcgg ctgcggcagg
     5401 agcgcatgag gagccgagag gacgaggatg cgctggagca ggcccggcgg gcccatgagg
     5461 aggcacgtcg gcgccaggag cagcagcagc agcagcgcca ggagcaacag cagcagcagc
     5521 aacagcaagc agctgcggtg gctgccgccg ccaccccaca ggcccagagc tcccagcccc
     5581 agtccatgct ggaccagcag agggagttgg cccggaagcg ggagcaggag cgaagacgcc
     5641 gggaagccat ggcagctacc attgacatga atttccagag tgatctattg tcaatatttg
     5701 aagaaaatct tttctgagcg gccgcactcg agatatctag acccagcttt cttgtacaaa
     5761 gtggttgata acgggaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     5821 ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga
     5881 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     5941 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     6001 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     6061 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc
     6121 gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     6181 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6241 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6301 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6361 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6421 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6481 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6541 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     6601 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6661 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6721 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6781 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6841 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6901 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6961 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7021 aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     7081 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     7141 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     7201 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     7261 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     7321 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     7381 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt
     7441 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     7501 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7561 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7681 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7741 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7801 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7861 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7921 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7981 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8221 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8281 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8341 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8401 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8461 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8521 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8581 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8641 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8761 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8821 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9061 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9901 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9961 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
    10021 gtcgctgagt agtgcgcgag caaaat
//
