LOCUS       sequence-44521-e        2914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2914)
  TITLE     Alpha Synuclein Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-7 asyn S87E/S129E
FEATURES             Location/Qualifiers
     source          1..2914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(42..833,834..902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     665..684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(903..1007)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1075..1093
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      1223..1811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1712..1731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1965..1982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2073..2092)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2235..2257
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2322..2341
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2322..2340
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2372..2394
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
       61 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      121 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      181 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      241 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      301 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
      361 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
      421 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
      481 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
      541 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
      601 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
      661 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
      721 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
      781 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
      841 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
      901 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      961 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1021 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1081 cgtatcacga ggccctttcg tcttcaagaa ttaaaaggat ctaggtgaag atcctttttg
     1141 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1201 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1261 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1321 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1381 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1441 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1501 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1561 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     1621 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1681 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1741 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1801 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1861 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1921 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1981 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2041 accgcataca gatctgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     2101 agccagtata tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     2161 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2221 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2281 cgcgaggccc agcgattcga acttctgata gacttcgaaa ttaatacgac tcactatagg
     2341 gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatataca
     2401 tatggatgta ttcatgaaag gactttcaaa ggccaaggag ggagttgtgg ctgctgctga
     2461 gaaaaccaaa cagggtgtgg cagaagcagc aggaaagaca aaagagggtg ttctctatgt
     2521 aggctccaaa accaaggagg gagtggtgca tggtgtggca acagtggctg agaagaccaa
     2581 agagcaagtg acaaatgttg gaggagcagt ggtgacgggt gtgacagcag tagcccagaa
     2641 gacagtggag ggagcagggg agattgcagc agccactggc tttgtcaaaa aggaccagtt
     2701 gggcaagaat gaagaaggag ccccacagga aggaattctg gaagatatgc ctgtggatcc
     2761 tgacaatgag gcttatgaaa tgcctgagga ggaagggtat caagactacg aacctgaagc
     2821 ctaagaaata tctttgctcc cagtttcttg agatctgctg acagatgttc catccaagct
     2881 tatcatcgat gataagctgt caaacatgag aatt
//
