LOCUS       sequence-445261-        7272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpT and GvpU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaTU
FEATURES             Location/Qualifiers
     source          1..7272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5530..5548)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5544..5591
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5658..5749
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6614..6653
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6684..7272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7173..7192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagatggga gaattactgt atttatacgg tttaattcca acaaaagaag
     3781 cagcagccat agagccgttt ccatcttata aggggtttga cggagaacat tcactgtacc
     3841 caattgcgtt tgatcaggtg acggctgtag tttctaagct ggatgctgac acctattcag
     3901 aaaaagtgat tcaagaaaaa atggagcagg atatgagctg gctgcaggaa aaagcatttc
     3961 atcatcacga aacggtagcc gctttgtacg aagaatttac gatcattcca ttaaaatttt
     4021 gcaccattta taaaggtgaa gaaagtctgc aggcagctat tgagattaac aaagaaaaga
     4081 tagagaattc actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa atttactgtg
     4141 atgatacaga gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa gcgaaaaaac
     4201 aagaaattag tcacttatca ccaggaagac agttttttga aaagaaaaaa atagatcagc
     4261 tgattgaaaa agaattagag cttcacaaaa acaaagtgtg tgaagagata catgacaagc
     4321 taaaagaatt atcgctttat gactctgtta aaaagaattg gagcaaagac gtaactggcg
     4381 cagctgaaca gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag attactaagt
     4441 tcgtaaacga aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg aagtttgaag
     4501 tgacgggacc atggccgccc tatcatttct cgagctttgc gtaaagtgag gaattaacat
     4561 tatgtctctt aaacaatcca tggagaataa agatattgct cttattgata ttttagatgt
     4621 cattttagat aaaggagtcg ccattaaagg agacttaatc atttccatag ctggcgtcga
     4681 tttagtgtat ttggatttgc gggtgcttat ttcttcggtt gaaacgcttg tgcaagcaaa
     4741 agaaggaaat cacaaaccaa tcacttctga acaatttgat aaacaaaagg aggaattaat
     4801 ggatgcaacc ggtcagccaa gcaaatggac gaatccactt ggatcctgat caagctgaac
     4861 aaggcttagc gcagcttgtg atgacagtta ttgagctatt gaggcaaatc gttgaacgtc
     4921 atgccatgag gcgggtggag ggtggaacgt tgacggacga acaaattgaa aacttaggaa
     4981 ttgcactaat gaacttagaa gaaaaaatgg acgagttgaa agaggtgttc ggtctggatg
     5041 cagaagattt aaatattgat cttggaccgc taggcagcct gctttaagcg gtcagtagga
     5101 ggaacagtat ggcagtcgaa cataatatgc agtcaagtac gattgtagat gtgctcgaaa
     5161 agattttgga taaaggagtc gttatagcgg gggacatcac cgtaggaatt gcagatgtcg
     5221 agctattaac gataaagatc cgcttgattg tggcttcggt tgataaggca aaagaaatcg
     5281 gcatggactg gtgggaaaat gatccgtatc tcagttcaaa aggagccaat aacaaagcgc
     5341 tcgaagaaga aaataaaatg ctgcatgagc ggttaaaaac gcttgaagaa aaaatagaaa
     5401 cgaaacgtta aaaactgtac gctacttcgt cacatttgct tctgcttgct gcagtccgga
     5461 ttcgcgaacg cgtctcgaga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     5521 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     5581 ggggtttttt gctgaaagga ggaactatat ccgactagta gcccgcctaa tgagcgggct
     5641 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5701 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5761 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5821 cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa ttgggggcac gagtgggata
     5881 catagaactg gacttgaata gcggtaaaat ccttgagagt tttcgccctg aagagcgttt
     5941 tccaatgatg agcactttca aagttctgct atgtggagca gtattatccc gtgtagatgc
     6001 ggggcaagag caactcggac gacgaataca ctattcgcag aatgacttgg ttgaatactc
     6061 cccagtgaca gaaaagcacc ttacggacgg aatgacggta agagaattat gtagtgccgc
     6121 cataacgatg agtgataaca ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa
     6181 ggaattaacc gcttttttgc acaatatggg agaccatgta actcgccttg accgttggga
     6241 accagaactg aatgaagcca taccaaacga cgagcgagac accacaatgc ctgcggcaat
     6301 ggcaacaaca ttacgcaaac tattaactgg cgaactactt actctggctt cacggcaaca
     6361 attaatagac tggcttgaag cggataaagt tgcaggacca ctactgcgtt cggcacttcc
     6421 tgctggctgg tttattgctg ataaatctgg ggcaggagag cgtggttcac ggggtatcat
     6481 tgccgcactt ggaccagatg gtaagccttc ccgtatcgta gttatctaca cgacgggtag
     6541 tcaggcaact atggacgaac gaaatagaca gattgctgaa ataggggctt cactgattaa
     6601 gcattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacggaccg
     6661 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6721 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6781 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6841 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6901 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6961 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7021 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7081 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7141 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7201 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7261 agcctatgga aa
//
