LOCUS       sequence-445263-        7310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpR and GvpL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBRL
FEATURES             Location/Qualifiers
     source          1..7310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2620..2637
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5568..5586)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5582..5629
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5696..5787
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6652..6691
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6722..7310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7211..7230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatc aggaggaacg taaaaatgac cgtcttaaca gacaaaagga
     1141 aaaaaggcag tggagctttt atacaagatg acgagacaaa agaggttctt tcaagagcgc
     1201 tgagctattt aaaatccggc tattccattc attttacagg tcctgccggc ggaggcaaaa
     1261 cctctttagc gcgagcgctt gctaaaaaga gaaagcgtcc tgtaatgctg atgcacggga
     1321 atcacgagct caacaacaaa gatttaattg gcgattttac gggatacacg agcaaaaaag
     1381 taatcgacca gtacgttcgt tctgtctata aaaaagatga acaggtgagt gaaaactggc
     1441 aggatggccg attgcttgaa gctgtaaaaa atggctatac gctgatttac gacgaattta
     1501 ctcgttctaa gcctgcgacg aataatatct ttctatcgat attagaagaa ggcgtgctgc
     1561 cgctgtatgg agtaaaaatg accgatcctt ttgtgcgcgt gcatcccgat ttccgcgtca
     1621 tcttcacaag caatccagct gagtatgccg gcgtatatga tacgcaagat gcgcttctcg
     1681 acaggttaat taccatgttt attgattata aagacatcga cagagagaca gcgattttaa
     1741 cggagaaaac ggacgtagaa gaagatgaag cgcgcacaat tgtaacgctc gtagcaaacg
     1801 tgcgaaaccg ctctggagac gaaaacagca gcggacttag cctgcgggct tcgcttatga
     1861 tcgctaccct tgccacgcag caagacattc ctatcgatgg aagtgacgaa gattttcaaa
     1921 cgttatgtat cgatattttg catcatccgc ttaccaaatg tttggatgaa gaaaatgcaa
     1981 aaagcaaagc cgaaaaaatc attttagaag aatgtaagaa tatagacact gaagaaaagt
     2041 aaaggagctt gaaaacatga gtgaaacaaa cgaaacaggt atttatattt ttagcgccat
     2101 tcaaacggat aaagacgaag aatttggcgc cgtggaagta gaaggaacaa aagctgaaac
     2161 atttttgatt cgctacaaag acgcggctat ggtagcagct gaagtaccga tgaaaattta
     2221 tcatcctaat cgccaaaatt tattaatgca tcaaaacgca gtagcagcga ttatggacaa
     2281 gaacgatacg gttattccaa tcagctttgg gaatgtattc aaatcaaaag aagacgtaaa
     2341 agttcttttg gaaaaccttt atccgcagtt tgaaaagctg tttccagcga tcaaaggaaa
     2401 aattgaagtc ggtttaaaag taattgggaa aaaagaatgg cttgagaaaa aagtaaacga
     2461 aaatcctgaa cttgagaaag tatcagcatc cgtaaaagga aaatcagaag cagccggtta
     2521 ttatgagcgt attcaacttg gaggaatggc tcaaaagatg tttacttccc tgcaaaaaga
     2581 agtcaagaca gatgtgtttt ctccgcttga agaagcagcg gaagcagcaa aagcaaatga
     2641 gccaacgggc gaaacgatgc ttttaaacgc gtctttctta attaaccgag aagatgaagc
     2701 gaagtttgat gaaaaagtaa atgaagcgca tgaaaactgg aaagacaaag ccgattttca
     2761 ttacagcggt ccttggcctg cttataattt tgtgaacatt cgcctaaaag tagaagagaa
     2821 ataacgtgct tcacaaatta gtaaccgcac ccattaacct tgtagtgaaa atcggcgaaa
     2881 aagtacagga agaagctgat aaacagctat atgaccttcc gacgattcag caaaagctca
     2941 ttcagcttca aatgatgttt gagcttggtg aaattccaga agaagcgttt caagaaaaag
     3001 aagatgaatt gttaatgagg tacgaaattg cgaaacgcag agaaattgaa caatgggaag
     3061 agctaacaca aaaaagaaat gaggaatcct agtgaggaat taacattatg tctcttaaac
     3121 aatccatgga gaataaagat attgctctta ttgatatttt agatgtcatt ttagataaag
     3181 gagtcgccat taaaggagac ttaatcattt ccatagctgg cgtcgattta gtgtatttgg
     3241 atttgcgggt gcttatttct tcggttgaaa cgcttgtgca agcaaaagaa ggaaatcaca
     3301 aaccaatcac ttctgaacaa tttgataaac aaaaggagga attaatggat gcaaccggtc
     3361 agccaagcaa atggacgaat ccacttggat cctgatcaag ctgaacaagg cttagcgcag
     3421 cttgtgatga cagttattga gctattgagg caaatcgttg aacgtcatgc catgaggcgg
     3481 gtggagggtg gaacgttgac ggacgaacaa attgaaaact taggaattgc actaatgaac
     3541 ttagaagaaa aaatggacga gttgaaagag gtgttcggtc tggatgcaga agatttaaat
     3601 attgatcttg gaccgctagg cagcctgctt taagcggtca gtaggaggaa cagtatggca
     3661 gtcgaacata atatgcagtc aagtacgatt gtagatgtgc tcgaaaagat tttggataaa
     3721 ggagtcgtta tagcggggga catcaccgta ggaattgcag atgtcgagct attaacgata
     3781 aagatccgct tgattgtggc ttcggttgat aaggcaaaag aaatcggcat ggactggtgg
     3841 gaaaatgatc cgtatctcag ttcaaaagga gccaataaca aagcgctcga agaagaaaat
     3901 aaaatgctgc atgagcggtt aaaaacgctt gaagaaaaaa tagaaacgaa acgttaaaaa
     3961 ctgtacgcta cttaaaaaat ggagggattt acaatggcaa ctgaaacaaa attagataac
     4021 acacaggcag aaaacaagga aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt
     4081 tcaaaagcaa gcaaaacaac aagcagcggg ccaatcaaac gagcggtagc aggaggcatc
     4141 atcggtgcaa cgattggata tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc
     4201 attgatacag acgaattaaa aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa
     4261 tcaaaaagca gcgtggccag cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa
     4321 gataaatcaa aagatgatga agaaaacgta aattcttcta gtagcgaaac agaagacgat
     4381 aacgttcaag agtacgacga gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca
     4441 cagcttgaag aaaaaatgaa catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa
     4501 gaagcggaag atacagattc cgacgaagaa gagaacgatg agaacgatga aaacgatgaa
     4561 aacgagcagg acgatgaaaa cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa
     4621 gaagctgagg aagaagaaag tgaaagtgac gaagacagcg aggaagaaga ggaagattct
     4681 cgctcaaaca aaaaaaataa aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa
     4741 gaagaaaaaa aggaagcgaa accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa
     4801 gcaaagaaaa acacggacga agaagatgat gaagcaacat ctctttctag tgaagacgat
     4861 acaacagcct aagacgtaaa ggaggaaaga aagacatgag tacaggccct tctttttcaa
     4921 ctaaagacaa tacgcttgaa tactttgtga aagcttctaa taaacacggc ttttcacttg
     4981 atatttcatt aaatgtaaac ggcgctgtga tttccggtac catgatttca gcaaaagaat
     5041 actttgatta cttaagcgaa acgtttgaag aaggcagtga agtggctcag gcgctaagcg
     5101 aacaattctc tttagcaagc gaagcgagcg aatcaaacgg agaagcagaa gcccatttta
     5161 ttcatttgaa aaatacaaag atttactgtg gagacagtaa atctactcct tctaaaggca
     5221 aaatcttttg gagagggaaa atagcagaag tagacgggtt tttcttagga aagatttctg
     5281 atgcaaaatc aacgagtaaa aagagttcat aaaaaacggc ggggtgattg ccccgccgtt
     5341 ttttagtgat gtgatgagat gtgcagcttc ttttttcgat acatgagcgg agtcatcttc
     5401 atttcttttc gaaactgatt aataaaatag cttgtactat taaaccctac ttgataggcg
     5461 acttccgtca catttgcttc tgcttgctgc agtccggatt cgcgaacgcg tctcgagaga
     5521 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     5581 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     5641 aactatatcc gactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
     5701 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5761 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5821 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta
     5881 aaagacgcag aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc
     5941 ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa
     6001 gttctgctat gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga
     6061 cgaatacact attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt
     6121 acggacggaa tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact
     6181 gcggcgaact tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac
     6241 aatatgggag accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata
     6301 ccaaacgacg agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta
     6361 ttaactggcg aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg
     6421 gataaagttg caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat
     6481 aaatctgggg caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt
     6541 aagccttccc gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga
     6601 aatagacaga ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt
     6661 aaaggctcct tttggagcct ttttttttgg acggaccggt agaaaagatc aaaggatctt
     6721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6841 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
//
