LOCUS       sequence-445266-        7942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpR and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBRS
FEATURES             Location/Qualifiers
     source          1..7942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2661..2678
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6200..6218)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6214..6261
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6328..6419
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7284..7323
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7354..7942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7843..7862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaaagatgg
     1141 caggaggaac gtaaaaatga ccgtcttaac agacaaaagg aaaaaaggca gtggagcttt
     1201 tatacaagat gacgagacaa aagaggttct ttcaagagcg ctgagctatt taaaatccgg
     1261 ctattccatt cattttacag gtcctgccgg cggaggcaaa acctctttag cgcgagcgct
     1321 tgctaaaaag agaaagcgtc ctgtaatgct gatgcacggg aatcacgagc tcaacaacaa
     1381 agatttaatt ggcgatttta cgggatacac gagcaaaaaa gtaatcgacc agtacgttcg
     1441 ttctgtctat aaaaaagatg aacaggtgag tgaaaactgg caggatggcc gattgcttga
     1501 agctgtaaaa aatggctata cgctgattta cgacgaattt actcgttcta agcctgcgac
     1561 gaataatatc tttctatcga tattagaaga aggcgtgctg ccgctgtatg gagtaaaaat
     1621 gaccgatcct tttgtgcgcg tgcatcccga tttccgcgtc atcttcacaa gcaatccagc
     1681 tgagtatgcc ggcgtatatg atacgcaaga tgcgcttctc gacaggttaa ttaccatgtt
     1741 tattgattat aaagacatcg acagagagac agcgatttta acggagaaaa cggacgtaga
     1801 agaagatgaa gcgcgcacaa ttgtaacgct cgtagcaaac gtgcgaaacc gctctggaga
     1861 cgaaaacagc agcggactta gcctgcgggc ttcgcttatg atcgctaccc ttgccacgca
     1921 gcaagacatt cctatcgatg gaagtgacga agattttcaa acgttatgta tcgatatttt
     1981 gcatcatccg cttaccaaat gtttggatga agaaaatgca aaaagcaaag ccgaaaaaat
     2041 cattttagaa gaatgtaaga atatagacac tgaagaaaag taaaggagct tgaaaacatg
     2101 agtgaaacaa acgaaacagg tatttatatt tttagcgcca ttcaaacgga taaagacgaa
     2161 gaatttggcg ccgtggaagt agaaggaaca aaagctgaaa catttttgat tcgctacaaa
     2221 gacgcggcta tggtagcagc tgaagtaccg atgaaaattt atcatcctaa tcgccaaaat
     2281 ttattaatgc atcaaaacgc agtagcagcg attatggaca agaacgatac ggttattcca
     2341 atcagctttg ggaatgtatt caaatcaaaa gaagacgtaa aagttctttt ggaaaacctt
     2401 tatccgcagt ttgaaaagct gtttccagcg atcaaaggaa aaattgaagt cggtttaaaa
     2461 gtaattggga aaaaagaatg gcttgagaaa aaagtaaacg aaaatcctga acttgagaaa
     2521 gtatcagcat ccgtaaaagg aaaatcagaa gcagccggtt attatgagcg tattcaactt
     2581 ggaggaatgg ctcaaaagat gtttacttcc ctgcaaaaag aagtcaagac agatgtgttt
     2641 tctccgcttg aagaagcagc ggaagcagca aaagcaaatg agccaacggg cgaaacgatg
     2701 cttttaaacg cgtctttctt aattaaccga gaagatgaag cgaagtttga tgaaaaagta
     2761 aatgaagcgc atgaaaactg gaaagacaaa gccgattttc attacagcgg tccttggcct
     2821 gcttataatt ttgtgaacat tcgcctaaaa gtagaagaga aataacgtgc ttcacaaatt
     2881 agtaaccgca cccattaacc ttgtagtgaa aatcggcgaa aaagtacagg aagaagctga
     2941 taaacagcta tatgaccttc cgacgattca gcaaaagctc attcagcttc aaatgatgtt
     3001 tgagcttggt gaaattccag aagaagcgtt tcaagaaaaa gaagatgaat tgttaatgag
     3061 gtacgaaatt gcgaaacgca gagaaattga acaatgggaa gagctaacac aaaaaagaaa
     3121 tgaggaatcc tagatgggag aattactgta tttatacggt ttaattccaa caaaagaagc
     3181 agcagccata gagccgtttc catcttataa ggggtttgac ggagaacatt cactgtaccc
     3241 aattgcgttt gatcaggtga cggctgtagt ttctaagctg gatgctgaca cctattcaga
     3301 aaaagtgatt caagaaaaaa tggagcagga tatgagctgg ctgcaggaaa aagcatttca
     3361 tcatcacgaa acggtagccg ctttgtacga agaatttacg atcattccat taaaattttg
     3421 caccatttat aaaggtgaag aaagtctgca ggcagctatt gagattaaca aagaaaagat
     3481 agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg aatgtgaaaa tttactgtga
     3541 tgatacagag cttaaaaaag gaatcagcga aacgaatgaa agcgtgaaag cgaaaaaaca
     3601 agaaattagt cacttatcac caggaagaca gttttttgaa aagaaaaaaa tagatcagct
     3661 gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt gaagagatac atgacaagct
     3721 aaaagaatta tcgctttatg actctgttaa aaagaattgg agcaaagacg taactggcgc
     3781 agctgaacag atggcgtgga acagcgtgtt tcttctcccg tctctgcaga ttactaagtt
     3841 cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat aaaggctgga agtttgaagt
     3901 gacgggacca tggccgccct atcatttctc gagctttccg taaaagcggt taagcgtgac
     3961 agtccaggag gaattaatgg atgcaaccgg tcagccaagc aaatggacga atccacttgg
     4021 atcctgatca agctgaacaa ggcttagcgc agcttgtgat gacagttatt gagctattga
     4081 ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg tggaacgttg acggacgaac
     4141 aaattgaaaa cttaggaatt gcactaatga acttagaaga aaaaatggac gagttgaaag
     4201 aggtgttcgg tctggatgca gaagatttaa atattgatct tggaccgcta ggcagcctgc
     4261 tttaagcggt cagtaggagg aacagtatgg cagtcgaaca taatatgcag tcaagtacga
     4321 ttgtagatgt gctcgaaaag attttggata aaggagtcgt tatagcgggg gacatcaccg
     4381 taggaattgc agatgtcgag ctattaacga taaagatccg cttgattgtg gcttcggttg
     4441 ataaggcaaa agaaatcggc atggactggt gggaaaatga tccgtatctc agttcaaaag
     4501 gagccaataa caaagcgctc gaagaagaaa ataaaatgct gcatgagcgg ttaaaaacgc
     4561 ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc tacttaaaaa atggagggat
     4621 ttacaatggc aactgaaaca aaattagata acacacaggc agaaaacaag gaaaataaaa
     4681 atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc aagcaaaaca acaagcagcg
     4741 ggccaatcaa acgagcggta gcaggaggca tcatcggtgc aacgattgga tatgtatcga
     4801 ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac agacgaatta aaaagcaaag
     4861 catctgattt aggaacaaag gtaaaagaaa aatcaaaaag cagcgtggcc agcctgaaaa
     4921 catctgcggg aagcttgttt aaaaaagata aagataaatc aaaagatgat gaagaaaacg
     4981 taaattcttc tagtagcgaa acagaagacg ataacgttca agagtacgac gagttaaaag
     5041 aagaaaatca aactcttcaa gatcgcttat cacagcttga agaaaaaatg aacatgcttg
     5101 ttgagcttag cctcaataaa aatcaagacg aagaagcgga agatacagat tccgacgaag
     5161 aagagaacga tgagaacgat gaaaacgatg aaaacgagca ggacgatgaa aacgaagaag
     5221 aaacatctaa gccacgtaaa aaggataaaa aagaagctga ggaagaagaa agtgaaagtg
     5281 acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa caaaaaaaat aaaaaagtaa
     5341 aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa aaaggaagcg aaaccaaaaa
     5401 agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa aaacacggac gaagaagatg
     5461 atgaagcaac atctctttct agtgaagacg atacaacagc ctaagacgta aaggaggaaa
     5521 gaaagacatg agtacaggcc cttctttttc aactaaagac aatacgcttg aatactttgt
     5581 gaaagcttct aataaacacg gcttttcact tgatatttca ttaaatgtaa acggcgctgt
     5641 gatttccggt accatgattt cagcaaaaga atactttgat tacttaagcg aaacgtttga
     5701 agaaggcagt gaagtggctc aggcgctaag cgaacaattc tctttagcaa gcgaagcgag
     5761 cgaatcaaac ggagaagcag aagcccattt tattcatttg aaaaatacaa agatttactg
     5821 tggagacagt aaatctactc cttctaaagg caaaatcttt tggagaggga aaatagcaga
     5881 agtagacggg tttttcttag gaaagatttc tgatgcaaaa tcaacgagta aaaagagttc
     5941 ataaaaaacg gcggggtgat tgccccgccg ttttttagtg atgtgatgag atgtgcagct
     6001 tcttttttcg atacatgagc ggagtcatct tcatttcttt tcgaaactga ttaataaaat
     6061 agcttgtact attaaaccct acttgatagg cgacttccgt cacatttgct tctgcttgct
     6121 gcagtccgga ttcgcgaacg cgtctcgaga gatccggctg ctaacaaagc ccgaaaggaa
     6181 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     6241 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccgactagta gcccgcctaa
     6301 tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt
     6361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6421 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6481 tttttgctca cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa ttgggggcac
     6541 gagtgggata catagaactg gacttgaata gcggtaaaat ccttgagagt tttcgccctg
     6601 aagagcgttt tccaatgatg agcactttca aagttctgct atgtggagca gtattatccc
     6661 gtgtagatgc ggggcaagag caactcggac gacgaataca ctattcgcag aatgacttgg
     6721 ttgaatactc cccagtgaca gaaaagcacc ttacggacgg aatgacggta agagaattat
     6781 gtagtgccgc cataacgatg agtgataaca ctgcggcgaa cttacttctg acaaccatcg
     6841 gtggaccgaa ggaattaacc gcttttttgc acaatatggg agaccatgta actcgccttg
     6901 accgttggga accagaactg aatgaagcca taccaaacga cgagcgagac accacaatgc
     6961 ctgcggcaat ggcaacaaca ttacgcaaac tattaactgg cgaactactt actctggctt
     7021 cacggcaaca attaatagac tggcttgaag cggataaagt tgcaggacca ctactgcgtt
     7081 cggcacttcc tgctggctgg tttattgctg ataaatctgg ggcaggagag cgtggttcac
     7141 ggggtatcat tgccgcactt ggaccagatg gtaagccttc ccgtatcgta gttatctaca
     7201 cgacgggtag tcaggcaact atggacgaac gaaatagaca gattgctgaa ataggggctt
     7261 cactgattaa gcattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt
     7321 ggacggaccg gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7381 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7441 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7501 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7561 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7621 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7681 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7741 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7801 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7861 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7921 aggggggcgg agcctatgga aa
//
