LOCUS       sequence-445268-       10420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging plasmid for TadCBEd in v3b PE-eVLP architecture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: P4-TadCBEd
FEATURES             Location/Qualifiers
     source          1..10420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             133..159
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             172..198
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             211..237
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             322..342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(940..963,964..966,967..5040)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             940..5040
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5071..5319
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5350..5598
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5641..5661
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5667..5686)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5732..5787
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5786..5805)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6145..6161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6145..6161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6158..6180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6169..6185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6193..6199,6200..6217,6218..6223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6238..6259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6376..6393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6547..7135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6627..6646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7306..8097,8098..8166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7929..7948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8167..8271)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8297..8752)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8434..8455)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8646..8665
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8879..8901
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8893..8910
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8894..8910
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8920..8939
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8920..8938
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        9059..9438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9439..9642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9593..9613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9639..9663
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          9754..10326
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgtctcccg aagataaaat agctcaactc aaacagaaaa ttcaggcttt gaaacaagaa
       61 aaccagcagt tggaggaaga aaatgcggcc ctggagtatg gcagtggcgg tagttctggc
      121 ggctcacttc aactgcctcc acttgaaaga ctgacactgg gatcattaca attacctcct
      181 ttagaacgat taacactcgg ttcactacag cttccgcctc ttgagagatt gacattaagt
      241 ggtggatcta cgtccacgct gctaatggag aactcgtctg gaggttctaa acggacagcc
      301 gacggaagcg agttcgagtc accaaagaag aagcggaaag tcagttctga ggtggagttt
      361 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgagagg
      421 aaggcgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      481 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatagccct gagacagggc
      541 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
      601 tgcgtgatgt gcgccggcgc catgatcaac tctaggatcg gccgcgtggt gtttggcgtg
      661 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggaatg
      721 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      781 gatttctatc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
      841 aactctggcg gatctagcgg aggatcctct ggcagcgaga caccaggaac aagcgagtca
      901 gcaacaccag agagcagtgg cggcagcagc ggcggcagcg acaagaagta cagcatcggc
      961 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     1021 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     1081 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     1141 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     1201 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     1261 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     1321 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     1381 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1441 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1501 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1561 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     1621 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1681 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1741 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1801 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1861 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     1921 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     1981 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     2041 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     2101 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     2161 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     2221 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     2281 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     2341 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     2401 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2461 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2521 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2581 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2641 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2701 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2761 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2821 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     2881 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     2941 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     3001 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     3061 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     3121 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     3181 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     3241 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     3301 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     3361 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3421 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     3481 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3541 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3601 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3661 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3721 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3781 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3841 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     3901 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     3961 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     4021 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     4081 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     4141 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     4201 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     4261 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     4321 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     4381 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     4441 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     4501 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     4561 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     4621 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     4681 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     4741 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     4801 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     4861 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     4921 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     4981 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac
     5041 agcggcggga gcggcgggag cggggggagc actaatctga gcgacatcat tgagaaggag
     5101 actgggaaac agctggtcat tcaggagtcc atcctgatgc tgcctgagga ggtggaggaa
     5161 gtgatcggca acaagccaga gtctgacatc ctggtgcaca ccgcctacga cgagtccaca
     5221 gatgagaatg tgatgctgct gacctctgac gcccccgagt ataagccttg ggccctggtc
     5281 atccaggatt ctaacggcga gaataagatc aagatgctga gcggaggatc cggaggatct
     5341 ggaggcagca ccaacctgtc tgacatcatc gagaaggaga caggcaagca gctggtcatc
     5401 caggagagca tcctgatgct gcccgaagaa gtcgaagaag tgatcggaaa caagcctgag
     5461 agcgatatcc tggtccatac cgcctacgac gagagtaccg acgaaaatgt gatgctgctg
     5521 acatccgacg ccccagagta taagccctgg gctctggtca tccaggattc caacggagag
     5581 aacaaaatca aaatgctgtc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     5641 cccaagaaga agaggaaagt ctgagatctt tttccctctg ccaaaaatta tggggacatc
     5701 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     5761 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     5821 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     5881 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     5941 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     6001 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     6061 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     6121 ggatcggccg caattcgtaa ccatgtcata gctgtttcct gtgtgaaatt gttatccgct
     6181 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6241 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6301 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8281 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8341 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8401 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8461 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8521 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8581 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8641 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8701 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     8761 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8821 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8881 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     8941 gaattggagc tccaccgcgg tggcggccgc tctagagagc ttggcccatt gcatacgttg
     9001 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga
     9061 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     9121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     9181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     9481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     9541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     9601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     9661 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     9721 agcctccggt cgaccgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct
     9781 ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg
     9841 tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt
     9901 cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact
     9961 ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg
    10021 tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat
    10081 tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt
    10141 ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg
    10201 gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata
    10261 aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc
    10321 ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc
    10381 ctcgagggcc ggatccgaat tcctgtattt gtctgaaaac
//
