LOCUS       sequence-44527-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6641 MSCV-P C-FlagHA 18E7-Kozak
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..88
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     104..120
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     148..167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        148..166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    196..220
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(536..560)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             592..615
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             631..657
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             631..657
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(705..728)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cctctgggta gccctttgtc accctaagcc tccgcctcct cttcctccat ccgccccgtc
       61 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
      121 tccttctcta ggcgccggaa ttagatctaa tacgactcac tatagggaga cccaagctgg
      181 ctagttaagc tatcaacaag tttgtacaaa aaagttggca ccatgcatgg acctaaggca
      241 acattgcaag acattgtatt gcatttagag ccccaaaatg aaattccggt tgaccttcta
      301 tgtcacgagc aattaagcga ctcagaggaa gaaaacgatg aaatagatgg agttaatcat
      361 caacatttac cagcccgacg agccgaacca caacgtcaca caatgttgtg tatgtgttgt
      421 aagtgtgaag ccagaattga gctagtagta gaaagctcag cagacgacct tcgagcattc
      481 cagcagctgt ttctgaacac cctgtccttt gtgtgtccgt ggtgtgcatc ccagcagcca
      541 actttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaactcga ggactacaag
      601 gacgacgatg acaagtcggc cgctggagga tacccctacg acgtgcccga ctacgcctag
      661 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      721 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      781 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      841 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      901 aagtagcacg
//
