LOCUS       sequence-445272-        7428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpF and GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBFG
FEATURES             Location/Qualifiers
     source          1..7428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..130
                     /gene="bla"
                     /label=AmpR promoter
     terminator      995..1034
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1065..1653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1554..1573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1807..1824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1839..1978
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1904..1923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2064..2086
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2080..2271)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2508..2527
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2508..2526
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7339..7357)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7353..7400
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agcccgccta atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca
      241 attgggggca cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag
      301 ttttcgccct gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc
      361 agtattatcc cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca
      421 gaatgacttg gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt
      481 aagagaatta tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct
      541 gacaaccatc ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt
      601 aactcgcctt gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga
      661 caccacaatg cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact
      721 tactctggct tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc
      781 actactgcgt tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga
      841 gcgtggttca cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt
      901 agttatctac acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga
      961 aataggggct tcactgatta agcattggta aaccgataca attaaaggct ccttttggag
     1021 cctttttttt tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt
     1081 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1141 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1201 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1261 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1321 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1381 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1441 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1501 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1561 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1621 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1681 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1741 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1801 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     1861 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     1921 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     1981 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2041 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2101 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     2161 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     2221 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     2281 cgtgtaaggg ggatttctgt tcatggggta atgataccga tgaaacgaga gaggatgctc
     2341 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     2401 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc atggtcaatg ccagcgcttc
     2461 gttaatacag atgtaggtgt tggcaccatg catcgatccc gcgaaattaa tacgactcac
     2521 tatagggaga ccacaacggt ttccctctag agatagctta gcgatgtgaa agaagctgga
     2581 gaaaactttc aagaaaaagt ttcttcttca gacgataaag agaagtcaaa aaataagcgg
     2641 aaaataaaag gcgtaaatca gattaaaaaa gcaacggata ttaaaagctc tactaagatt
     2701 aaaagttcta atgatattaa atcatctaca gatttaaaaa caatgggatc attagatcta
     2761 actattggag gctactaaaa atggaaatta aaaaaattat gcaagccgtg aacgactttt
     2821 tcggtgaaca cgtagctcct cctcataaaa ttacctcggt ggaagctact gaagatgaag
     2881 gttggagagt tattgttgaa gtcattgaag aacgagaata tatgaaaaaa tacgccaaag
     2941 atgaaatgct cggaacgtac gagtgctttg taaataaaga aaaagaagtc atttcattca
     3001 aacgactcga cgtcagatat agaagcgcca ttggcattga agcataaacg ataagatggc
     3061 aggaggaacg taaaaatgac cgtcttaaca gacaaaagga aaaaaggcag tggagctttt
     3121 atacaagatg acgagacaaa agaggttctt tcaagagcgc tgagctattt aaaatccggc
     3181 tattccattc attttacagg tcctgccggc ggaggcaaaa cctctttagc gcgagcgctt
     3241 gctaaaaaga gaaagcgtcc tgtaatgctg atgcacggga atcacgagct caacaacaaa
     3301 gatttaattg gcgattttac gggatacacg agcaaaaaag taatcgacca gtacgttcgt
     3361 tctgtctata aaaaagatga acaggtgagt gaaaactggc aggatggccg attgcttgaa
     3421 gctgtaaaaa atggctatac gctgatttac gacgaattta ctcgttctaa gcctgcgacg
     3481 aataatatct ttctatcgat attagaagaa ggcgtgctgc cgctgtatgg agtaaaaatg
     3541 accgatcctt ttgtgcgcgt gcatcccgat ttccgcgtca tcttcacaag caatccagct
     3601 gagtatgccg gcgtatatga tacgcaagat gcgcttctcg acaggttaat taccatgttt
     3661 attgattata aagacatcga cagagagaca gcgattttaa cggagaaaac ggacgtagaa
     3721 gaagatgaag cgcgcacaat tgtaacgctc gtagcaaacg tgcgaaaccg ctctggagac
     3781 gaaaacagca gcggacttag cctgcgggct tcgcttatga tcgctaccct tgccacgcag
     3841 caagacattc ctatcgatgg aagtgacgaa gattttcaaa cgttatgtat cgatattttg
     3901 catcatccgc ttaccaaatg tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc
     3961 attttagaag aatgtaagaa tatagacact gaagaaaagt aagtaggaac aatgggaaga
     4021 gctaacacaa aaaagaaatg aggaatccta gatgggagaa ttactgtatt tatacggttt
     4081 aattccaaca aaagaagcag cagccataga gccgtttcca tcttataagg ggtttgacgg
     4141 agaacattca ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt ctaagctgga
     4201 tgctgacacc tattcagaaa aagtgattca agaaaaaatg gagcaggata tgagctggct
     4261 gcaggaaaaa gcatttcatc atcacgaaac ggtagccgct ttgtacgaag aatttacgat
     4321 cattccatta aaattttgca ccatttataa aggtgaagaa agtctgcagg cagctattga
     4381 gattaacaaa gaaaagatag agaattcact gacgctgctt caaggaaatg aagagtggaa
     4441 tgtgaaaatt tactgtgatg atacagagct taaaaaagga atcagcgaaa cgaatgaaag
     4501 cgtgaaagcg aaaaaacaag aaattagtca cttatcacca ggaagacagt tttttgaaaa
     4561 gaaaaaaata gatcagctga ttgaaaaaga attagagctt cacaaaaaca aagtgtgtga
     4621 agagatacat gacaagctaa aagaattatc gctttatgac tctgttaaaa agaattggag
     4681 caaagacgta actggcgcag ctgaacagat ggcgtggaac agcgtgtttc ttctcccgtc
     4741 tctgcagatt actaagttcg taaacgaaat agaagagctt cagcaaaggc ttgaaaataa
     4801 aggctggaag tttgaagtga cgggaccatg gccgccctat catttctcga gctttgcgta
     4861 aagtgaggaa ttaacattat gtctcttaaa caatccatgg agaataaaga tattgctctt
     4921 attgatattt tagatgtcat tttagataaa ggagtcgcca ttaaaggaga cttaatcatt
     4981 tccatagctg gcgtcgattt agtgtatttg gatttgcggg tgcttatttc ttcggttgaa
     5041 acgcttgtgc aagcaaaaga aggaaatcac aaaccaatca cttctgaaca atttgataaa
     5101 caaaaggagg aattaatgga tgcaaccggt cagccaagca aatggacgaa tccacttgga
     5161 tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg acagttattg agctattgag
     5221 gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga cggacgaaca
     5281 aattgaaaac ttaggaattg cactaatgaa cttagaagaa aaaatggacg agttgaaaga
     5341 ggtgttcggt ctggatgcag aagatttaaa tattgatctt ggaccgctag gcagcctgct
     5401 ttaagcggtc agtaggagga acagtatggc agtcgaacat aatatgcagt caagtacgat
     5461 tgtagatgtg ctcgaaaaga ttttggataa aggagtcgtt atagcggggg acatcaccgt
     5521 aggaattgca gatgtcgagc tattaacgat aaagatccgc ttgattgtgg cttcggttga
     5581 taaggcaaaa gaaatcggca tggactggtg ggaaaatgat ccgtatctca gttcaaaagg
     5641 agccaataac aaagcgctcg aagaagaaaa taaaatgctg catgagcggt taaaaacgct
     5701 tgaagaaaaa atagaaacga aacgttaaaa actgtacgct acttaaaaaa tggagggatt
     5761 tacaatggca actgaaacaa aattagataa cacacaggca gaaaacaagg aaaataaaaa
     5821 tgcggaaaac ggttcaaaag aaaagaacgg ttcaaaagca agcaaaacaa caagcagcgg
     5881 gccaatcaaa cgagcggtag caggaggcat catcggtgca acgattggat atgtatcgac
     5941 tcctgaaaat cgaaaaagtc tccttgaccg cattgataca gacgaattaa aaagcaaagc
     6001 atctgattta ggaacaaagg taaaagaaaa atcaaaaagc agcgtggcca gcctgaaaac
     6061 atctgcggga agcttgttta aaaaagataa agataaatca aaagatgatg aagaaaacgt
     6121 aaattcttct agtagcgaaa cagaagacga taacgttcaa gagtacgacg agttaaaaga
     6181 agaaaatcaa actcttcaag atcgcttatc acagcttgaa gaaaaaatga acatgcttgt
     6241 tgagcttagc ctcaataaaa atcaagacga agaagcggaa gatacagatt ccgacgaaga
     6301 agagaacgat gagaacgatg aaaacgatga aaacgagcag gacgatgaaa acgaagaaga
     6361 aacatctaag ccacgtaaaa aggataaaaa agaagctgag gaagaagaaa gtgaaagtga
     6421 cgaagacagc gaggaagaag aggaagattc tcgctcaaac aaaaaaaata aaaaagtaaa
     6481 aacagaagaa gaagacgaag atgaaagcga agaagaaaaa aaggaagcga aaccaaaaaa
     6541 gtcaacagct aaaaaatcaa aaaatacaaa agcaaagaaa aacacggacg aagaagatga
     6601 tgaagcaaca tctctttcta gtgaagacga tacaacagcc taagacgtaa aggaggaaag
     6661 aaagacatga gtacaggccc ttctttttca actaaagaca atacgcttga atactttgtg
     6721 aaagcttcta ataaacacgg cttttcactt gatatttcat taaatgtaaa cggcgctgtg
     6781 atttccggta ccatgatttc agcaaaagaa tactttgatt acttaagcga aacgtttgaa
     6841 gaaggcagtg aagtggctca ggcgctaagc gaacaattct ctttagcaag cgaagcgagc
     6901 gaatcaaacg gagaagcaga agcccatttt attcatttga aaaatacaaa gatttactgt
     6961 ggagacagta aatctactcc ttctaaaggc aaaatctttt ggagagggaa aatagcagaa
     7021 gtagacgggt ttttcttagg aaagatttct gatgcaaaat caacgagtaa aaagagttca
     7081 taaaaaacgg cggggtgatt gccccgccgt tttttagtga tgtgatgaga tgtgcagctt
     7141 cttttttcga tacatgagcg gagtcatctt catttctttt cgaaactgat taataaaata
     7201 gcttgtacta ttaaacccta cttgataggc gacttccgtc acatttgctt ctgcttgctg
     7261 cagtccggat tcgcgaacgc gtctcgagag atccggctgc taacaaagcc cgaaaggaag
     7321 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     7381 gggtcttgag gggttttttg ctgaaaggag gaactatatc cgactagt
//
