LOCUS       sequence-445277-        8329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpG and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaGK
FEATURES             Location/Qualifiers
     source          1..8329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6587..6605)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6601..6648
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6715..6806
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7671..7710
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7741..8329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8230..8249
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgaa atgaggaatc
     3481 ctagatggga gaattactgt atttatacgg tttaattcca acaaaagaag cagcagccat
     3541 agagccgttt ccatcttata aggggtttga cggagaacat tcactgtacc caattgcgtt
     3601 tgatcaggtg acggctgtag tttctaagct ggatgctgac acctattcag aaaaagtgat
     3661 tcaagaaaaa atggagcagg atatgagctg gctgcaggaa aaagcatttc atcatcacga
     3721 aacggtagcc gctttgtacg aagaatttac gatcattcca ttaaaatttt gcaccattta
     3781 taaaggtgaa gaaagtctgc aggcagctat tgagattaac aaagaaaaga tagagaattc
     3841 actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa atttactgtg atgatacaga
     3901 gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa gcgaaaaaac aagaaattag
     3961 tcacttatca ccaggaagac agttttttga aaagaaaaaa atagatcagc tgattgaaaa
     4021 agaattagag cttcacaaaa acaaagtgtg tgaagagata catgacaagc taaaagaatt
     4081 atcgctttat gactctgtta aaaagaattg gagcaaagac gtaactggcg cagctgaaca
     4141 gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag attactaagt tcgtaaacga
     4201 aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg aagtttgaag tgacgggacc
     4261 atggccgccc tatcatttct cgagctttgc gtaaagtgag gaattaacat tatgtctctt
     4321 aaacaatcca tggagaataa agatattgct cttattgata ttttagatgt cattttagat
     4381 aaaggagtcg ccattaaagg agacttaatc atttccatag ctggcgtcga tttagtgtat
     4441 ttggatttgc gggtgcttat ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat
     4501 cacaaaccaa tcacttctga acaatttgat aaacaaaaag aagagctgat ggacgctacg
     4561 ggccagccta gtaagtggac gaaccctcta ggttcgtgac ccttgcgagc agtcgtatgc
     4621 tttctcgaat tccgagcggt taagcgtgac aggcggtcag taggaggaac agtatggcag
     4681 tcgaacataa tatgcagtca agtacgattg tagatgtgct cgaaaagatt ttggataaag
     4741 gagtcgttat agcgggggac atcaccgtag gaattgcaga tgtcgagcta ttaacgataa
     4801 agatccgctt gattgtggct tcggttgata aggcaaaaga aatcggcatg gactggtggg
     4861 aaaatgatcc gtatctcagt tcaaaaggag ccaataacaa agcgctcgaa gaagaaaata
     4921 aaatgctgca tgagcggtta aaaacgcttg aagaaaaaat agaaacgaaa cgttaaaaac
     4981 tgtacgctac ttaaaaaatg gagggattta caatggcaac tgaaacaaaa ttagataaca
     5041 cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg ttcaaaagaa aagaacggtt
     5101 caaaagcaag caaaacaaca agcagcgggc caatcaaacg agcggtagca ggaggcatca
     5161 tcggtgcaac gattggatat gtatcgactc ctgaaaatcg aaaaagtctc cttgaccgca
     5221 ttgatacaga cgaattaaaa agcaaagcat ctgatttagg aacaaaggta aaagaaaaat
     5281 caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag cttgtttaaa aaagataaag
     5341 ataaatcaaa agatgatgaa gaaaacgtaa attcttctag tagcgaaaca gaagacgata
     5401 acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac tcttcaagat cgcttatcac
     5461 agcttgaaga aaaaatgaac atgcttgttg agcttagcct caataaaaat caagacgaag
     5521 aagcggaaga tacagattcc gacgaagaag agaacgatga gaacgatgaa aacgatgaaa
     5581 acgagcagga cgatgaaaac gaagaagaaa catctaagcc acgtaaaaag gataaaaaag
     5641 aagctgagga agaagaaagt gaaagtgacg aagacagcga ggaagaagag gaagattctc
     5701 gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga agacgaagat gaaagcgaag
     5761 aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa aaaatcaaaa aatacaaaag
     5821 caaagaaaaa cacggacgaa gaagatgatg aagcaacatc tctttctagt gaagacgata
     5881 caacagccta agacgtaaag gaggaaagaa agacatgagt acaggccctt ctttttcaac
     5941 taaagacaat acgcttgaat actttgtgaa agcttctaat aaacacggct tttcacttga
     6001 tatttcatta aatgtaaacg gcgctgtgat ttccggtacc atgatttcag caaaagaata
     6061 ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa gtggctcagg cgctaagcga
     6121 acaattctct ttagcaagcg aagcgagcga atcaaacgga gaagcagaag cccattttat
     6181 tcatttgaaa aatacaaaga tttactgtgg agacagtaaa tctactcctt ctaaaggcaa
     6241 aatcttttgg agagggaaaa tagcagaagt agacgggttt ttcttaggaa agatttctga
     6301 tgcaaaatca acgagtaaaa agagttcata aaaaacggcg gggtgattgc cccgccgttt
     6361 tttagtgatg tgatgagatg tgcagcttct tttttcgata catgagcgga gtcatcttca
     6421 tttcttttcg aaactgatta ataaaatagc ttgtactatt aaaccctact tgataggcga
     6481 cttccgtcac atttgcttct gcttgctgca gtccggattc gcgaacgcgt ctcgagagat
     6541 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     6601 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     6661 actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
     6721 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6781 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6841 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa
     6901 aagacgcaga ggaccaattg ggggcacgag tgggatacat agaactggac ttgaatagcg
     6961 gtaaaatcct tgagagtttt cgccctgaag agcgttttcc aatgatgagc actttcaaag
     7021 ttctgctatg tggagcagta ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac
     7081 gaatacacta ttcgcagaat gacttggttg aatactcccc agtgacagaa aagcacctta
     7141 cggacggaat gacggtaaga gaattatgta gtgccgccat aacgatgagt gataacactg
     7201 cggcgaactt acttctgaca accatcggtg gaccgaagga attaaccgct tttttgcaca
     7261 atatgggaga ccatgtaact cgccttgacc gttgggaacc agaactgaat gaagccatac
     7321 caaacgacga gcgagacacc acaatgcctg cggcaatggc aacaacatta cgcaaactat
     7381 taactggcga actacttact ctggcttcac ggcaacaatt aatagactgg cttgaagcgg
     7441 ataaagttgc aggaccacta ctgcgttcgg cacttcctgc tggctggttt attgctgata
     7501 aatctggggc aggagagcgt ggttcacggg gtatcattgc cgcacttgga ccagatggta
     7561 agccttcccg tatcgtagtt atctacacga cgggtagtca ggcaactatg gacgaacgaa
     7621 atagacagat tgctgaaata ggggcttcac tgattaagca ttggtaaacc gatacaatta
     7681 aaggctcctt ttggagcctt tttttttgga cggaccggta gaaaagatca aaggatcttc
     7741 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7801 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7861 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7921 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7981 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8041 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8101 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8161 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8221 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8281 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa
//
