LOCUS       sequence-445288-        7990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpG and GvpU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaGU
FEATURES             Location/Qualifiers
     source          1..7990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6248..6266)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6262..6309
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6376..6467
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7332..7371
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7402..7990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7891..7910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgaa atgaggaatc
     3481 ctagatggga gaattactgt atttatacgg tttaattcca acaaaagaag cagcagccat
     3541 agagccgttt ccatcttata aggggtttga cggagaacat tcactgtacc caattgcgtt
     3601 tgatcaggtg acggctgtag tttctaagct ggatgctgac acctattcag aaaaagtgat
     3661 tcaagaaaaa atggagcagg atatgagctg gctgcaggaa aaagcatttc atcatcacga
     3721 aacggtagcc gctttgtacg aagaatttac gatcattcca ttaaaatttt gcaccattta
     3781 taaaggtgaa gaaagtctgc aggcagctat tgagattaac aaagaaaaga tagagaattc
     3841 actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa atttactgtg atgatacaga
     3901 gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa gcgaaaaaac aagaaattag
     3961 tcacttatca ccaggaagac agttttttga aaagaaaaaa atagatcagc tgattgaaaa
     4021 agaattagag cttcacaaaa acaaagtgtg tgaagagata catgacaagc taaaagaatt
     4081 atcgctttat gactctgtta aaaagaattg gagcaaagac gtaactggcg cagctgaaca
     4141 gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag attactaagt tcgtaaacga
     4201 aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg aagtttgaag tgacgggacc
     4261 atggccgccc tatcatttct cgagctttgc gtaaagtgag gaattaacat tatgtctctt
     4321 aaacaatcca tggagaataa agatattgct cttattgata ttttagatgt cattttagat
     4381 aaaggagtcg ccattaaagg agacttaatc atttccatag ctggcgtcga tttagtgtat
     4441 ttggatttgc gggtgcttat ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat
     4501 cacaaaccaa tcacttctga acaatttgat aaacaaaagg aggaattaat ggatgcaacc
     4561 ggtcagccaa gcaaatggac gaatccactt ggatcctgat caggaggaat taatggatgc
     4621 aaccggtcag ccaagcaaat ggacgaatcc acttggatcc tgatcaagct gaacaaggct
     4681 tagcgcagct tgtgatgaca gttattgagc tattgaggca aatcgttgaa cgtcatgcca
     4741 tgaggcgggt ggagggtgga acgttgacgg acgaacaaat tgaaaactta ggaattgcac
     4801 taatgaactt agaagaaaaa atggacgagt tgaaagaggt gttcggtctg gatgcagaag
     4861 atttaaatat tgatcttgga ccgctaggca gcctgcttta agcggtcagt aggaggaaca
     4921 gtatggcagt cgaacataat atgcagtcaa gtacgattgt agatgtgctc gaaaagattt
     4981 tggataaagg agtcgttata gcgggggaca tcaccgtagg aattgcagat gtcgagctat
     5041 taacgataaa gatccgcttg attgtggctt cggttgataa ggcaaaagaa atcggcatgg
     5101 actggtggga aaatgatccg tatctcagtt caaaaggagc caataacaaa gcgctcgaag
     5161 aagaaaataa aatgctgcat gagcggttaa aaacgcttga agaaaaaata gaaacgaaac
     5221 gttaaaaact gtacgctact taaaaaatgg agggatttac aatggcaact gaaacaaaat
     5281 tagataacac acaggcagaa aacaaggaaa ataaaaatgc ggaaaacggt tcaaaagaaa
     5341 agaacggttc aaaagcaagc aaaacaacaa gcagcgggcc aatcaaacga gcggtagcag
     5401 gaggcatcat cggtgcaacg attggatatg tatcgactcc tgaaaatcga aaaagtctcc
     5461 ttgaccgcat tgatacagac gaattaaaaa gcaaagcatc tgatttagga acaaaggtaa
     5521 aagaaaaatc aaaaagcagc gtggccagcc tgaaaacatc tgcgggaagc ttgtttaaaa
     5581 aagataaaga taaatcaaaa gatgatgaag aaaacgtaaa ttcttctagt agcgaaacag
     5641 aagacgataa cgttcaagag tacgacgagt taaaagaaga aaatcaaact cttcaagatc
     5701 gcttatcaca gcttgaagaa aaaatgaaca tgcttgttga gcttagcctc aataaaaatc
     5761 aagacgaaga agcggaagat acagattccg acgaagaaga gaacgatgag aacgatgaaa
     5821 acgatgaaaa cgagcaggac gatgaaaacg aagaagaaac atctaagcca cgtaaaaagg
     5881 ataaaaaaga agctgaggaa gaagaaagtg aaagtgacga agacagcgag gaagaagagg
     5941 aagattctcg ctcaaacaaa aaaaataaaa aagtaaaaac agaagaagaa gacgaagatg
     6001 aaagcgaaga agaaaaaaag gaagcgaaac caaaaaagtc aacagctaaa aaatcaaaaa
     6061 atacaaaagc aaagaaaaac acggacgaag aagatgatga agcaacatct ctttctagtg
     6121 aagacgatac aacagcctaa gacgtcgtca catttgcttc tgcttgctgc agtccggatt
     6181 cgcgaacgcg tctcgagaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     6241 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     6301 ggttttttgc tgaaaggagg aactatatcc gactagtagc ccgcctaatg agcgggcttt
     6361 tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6541 cagaaacgct cgtgaaagta aaagacgcag aggaccaatt gggggcacga gtgggataca
     6601 tagaactgga cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc
     6661 caatgatgag cactttcaaa gttctgctat gtggagcagt attatcccgt gtagatgcgg
     6721 ggcaagagca actcggacga cgaatacact attcgcagaa tgacttggtt gaatactccc
     6781 cagtgacaga aaagcacctt acggacggaa tgacggtaag agaattatgt agtgccgcca
     6841 taacgatgag tgataacact gcggcgaact tacttctgac aaccatcggt ggaccgaagg
     6901 aattaaccgc ttttttgcac aatatgggag accatgtaac tcgccttgac cgttgggaac
     6961 cagaactgaa tgaagccata ccaaacgacg agcgagacac cacaatgcct gcggcaatgg
     7021 caacaacatt acgcaaacta ttaactggcg aactacttac tctggcttca cggcaacaat
     7081 taatagactg gcttgaagcg gataaagttg caggaccact actgcgttcg gcacttcctg
     7141 ctggctggtt tattgctgat aaatctgggg caggagagcg tggttcacgg ggtatcattg
     7201 ccgcacttgg accagatggt aagccttccc gtatcgtagt tatctacacg acgggtagtc
     7261 aggcaactat ggacgaacga aatagacaga ttgctgaaat aggggcttca ctgattaagc
     7321 attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg acggaccggt
     7381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7981 cctatggaaa
//
