LOCUS       sequence-445302-       14534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 based vector for optimised episomal expression in filamentous 
            fungi, with increased selection stringency using the modified 
            ubi-Y-pyrG marker (high stringency)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14534)
  AUTHORS   Roux I, Woodcraft C, Sbaraini N, Pepper A, Wong E, Bracegirdle J, 
            Chooi YH
  TITLE     Next-generation AMA1-based plasmids for enhanced heterologous 
            expression in filamentous fungi.
  JOURNAL   Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 
            10.1111/1751-7915.70010.
  PUBMED    39276061
REFERENCE   2  (bases 1 to 14534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol."; date: "2024-09"; volume: "17(9):e70010. doi"; pages: "
            10.1111/1751-7915.70010"
COMMENT     Sequence Label: pYFAC-ubi-Y-pyrG
FEATURES             Location/Qualifiers
     source          1..14534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2803..2824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             4384..4611
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             join(6619..6687,6688..7479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6837..6856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7650..8238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8139..8158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8392..8409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8526..8547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8562..8567,8568..8585,8586..8592)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8600..8616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8605..8627
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8624..8640
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8624..8640
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      8657..9999
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9620..9667)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10040..10058
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10096..10118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10218..10237
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10261..10476
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10477..11280
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(11562..11579)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11562..11578)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11571..11593)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11878..11897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      join(11919..14534,1..2633)
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 ctgcagattt tgacctagtt aacaaggcac atcatgctat cacttccaac actcttgctg
       61 agcgcgttct gccttgcctt tctggttcta cgaagtatcc agtcccgcct gcaacgccgc
      121 agaaacgcca aaaggtgggg ttgtcaaccg ccagctagag ctccatcggg attctttggc
      181 attccgccgt tcctcggtgg tcgcagataa ctccctaaat tccctgagct aactctaagt
      241 cgaccatgcc gtttatggtt agcgcctccc aaaaaggaat ggccgactta gagttacctc
      301 ttgaccgact ttttctttcc tcccccttac atttcgttac cacaacacat tcctatatcc
      361 aaactccagg tacataacta gtcgaaatct cttttaaatc tagtcaagaa ctagatactt
      421 aaccttcata ctgcttagag atagcctttg aagctcttat ttcgctcttt gtctcactct
      481 caccttcctc ctcctcctcc aatccttttt gctcgggact agtccaagac tagtcaacga
      541 ttagtatgcc gagcattcgc gataacgatc tccgaaagtc cccagagtac tgtcactatc
      601 tcgaggcagt taaggacggg gagcttacgc tgccggattt caagatagta agccgacccg
      661 actagcttta agcctagtta ctgactagtc cacaggacga caatggcgtg cctgatatcc
      721 atccatatga agtctactgc cgagtgaagg gatgcctcaa gcgtacagtg agtctactgc
      781 tatactagtt tctgactagt tattaactag ttgacaggtt ccctctgcca acagaaacat
      841 attggtcaag cacttgaagg acaagaactc ccacggcatg gagtttacat tgcacaatgg
      901 acctcccact atgaaggaac tgatggaggc caaaggcaag tcctatctag attacttggt
      961 gactagtccc tgactagtca agtagcatgg tatgaaggct tgtttgaagg cactgttctc
     1021 ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt aagtttctgt gagtctaact
     1081 agtgtattag ctaatatata gcagcaccaa gtccaaggac cacaatacta agggtgtcga
     1141 gaattcgtga gtttcttctc ccatttcaac tagtccttga ctagtcacta actactttgc
     1201 agcaacgagg gtgaagctgg aaatgatcag gacaatggcg agggcccgta agtacagcca
     1261 ttcaatgcag actagttgct aactaatctg tgactagttc aagtggtccg tacgccgtgc
     1321 atacccctgt gactggtagg aatttgagca agcctgtctt gccgcgcgat gagaaaggaa
     1381 aggcaagtta cattcagccc cgtacctagg atcagtgcta atttataacc tctagccact
     1441 ctttatgcag atccgccgtg agggtagcaa ggcagctaaa tcagctggtg agaaaggaac
     1501 cataccctgc aagacctgtc gcaacgcaaa gggcaaaggt aagctatcca agctagtttg
     1561 ggactagatt ctaactagtc tcagcaccgt gtggttcaaa gccatattgc gagttttggc
     1621 gctttttctc atcgattgac gaggcaaagg gagcgagtat gcaacctcgt aagtcagact
     1681 cagacaaaga ccaactagtt attgaccagt cttctagaag gctctgttgt ggatcttgag
     1741 gccctggaga gttcctccaa caatccggag acaagcaagt cctcgtcgga ctagtcacta
     1801 actagactct aactagttgc agacatggat aatgcaaaag agacaagcaa tgaagaaagt
     1861 ggtaagacat ttctcctttg tggttctgga ctagtctttg actagtcaca gtcttaaaca
     1921 aggaaaatga gcatgaaaat gaggaggaaa aggctgctga gcccgaggaa gtgcagggtg
     1981 atggcagaca tggtaggtta ataccttgtt agttattgct agtcactgac tagtcaataa
     2041 ctagtctctg aacaccttgc aatcactccg tttgcgcagc tgaacagtgg tgaggataat
     2101 agtagtaagt tattctagct tcagagttat aggagactag atactaacta gtattagttg
     2161 caactaacct ggatctcaga gactttggcc tcaatctaga atctatctag ttgtcaacta
     2221 gactgtggta tcattgtctt ttattttcct agtcctggaa ctagcttcta actagtctcc
     2281 ctaatatgtg gctgtcttgt tttttttttt tgtttcccta cccggatatc tagtcccctt
     2341 ctaggttctg ttaacctctc gggctctgat ttagtttaac gcaaacctga gattagtttc
     2401 taactagtct ctaggttttc tatccacctt taattgtaat aataaataca agcaacgttt
     2461 atacgtcaaa agcatttata aacttttacc ctaaagtagc ttgcttgtgt gtttagttta
     2521 taattagtct cttattaatt tgatgtaggt aagcccgcca caaatatata tttttaacaa
     2581 gataccgtgg aaaaacttcg tgctatcaca aaacagtata caaaaaataa gctggctttc
     2641 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2701 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2761 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2821 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     2881 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2941 aaatggggtg acgatgagcc gctcttgcat ctttgtttgt attatactgt ctttcttgtt
     3001 acacataatt attctagaat gccccaccgt tacatacggg acacagccat ttacatatgc
     3061 atgtggatta cgagctaacg agttcattca aatctcagaa ctatcacata atcatcattc
     3121 ccctatcgtc aaagaccgta agacaaatcc ggttcatgca ctgaacccat tcgggtagtg
     3181 agtcatttac tcagcacact cgcgctgacg ctcgtcgaac accttcaatg cctcctcggc
     3241 agccttgaca ccactgagaa ccatggcacc gaaggtaggg cccatgcggt taaagccatc
     3301 aatttcagac agctccatac cgccgattat caagccctta gtaacctcgc gggtgttctt
     3361 gacgatggca tcctgcggcc gcccagaaaa ttacgggtat gtcctccact gtgttgaagt
     3421 cagaaaaaca atcgataatt tgaagctcat taaaatgcca gttccgtcgt cgccgttgcc
     3481 ctaggagcta ttccagtgct tagcttcatt catggatgta tcgtgactgg ccttcggaag
     3541 gatgccaagg tcccctatcc gcacagctat gcgaccgttg agcaatgcaa gtcaaatgtg
     3601 tgcatacctg gtctttggtt tggtacaccg ctatgtgaag attattaatg ctgaagtcat
     3661 gatacaggcc aaagccgagc aatttaactg cgcgcagcgg gcccacgcta actacctcga
     3721 gaatatgcct caaacaatgc tcttcaccct cttcgcgggt ctgaaatacc ctcacctggc
     3781 aacagcaatt ggcgcttcat ggctgttttt ccgatctctc tacttgtacg gctatgtgta
     3841 ctcgggtaag ccacaaggca agggcagatt gctgggaggt ttcttctggt tttctcaagg
     3901 cgctctgtgg gctctgagtg tgtttggtgt tgccaaagac atgatctctt actgagagtt
     3961 attctgtgtc tgacgaaata tgttgtgtat atatatatat gtacgttaaa agttccgtgg
     4021 agttaccagt gattgaccaa tgttttatct tctacagttc tgcctgtcta ccccattcta
     4081 gctgtacctg actacagagt agtttaattg tggttgaccc cacagtcgga ggcggaggaa
     4141 tacagcaccg atgtggcctg tctccatcca gattggcacg caatttttac acgcggaaaa
     4201 gatcgagata gagtacgact ttaaatttag tccccggcgg cttctatttt agaatatttg
     4261 agatttgatt ctcaagcaat tgatttggtt gggtcaccct caattggata atatacctca
     4321 ttgctcggct acttcaactc atcaatcacc gtcatacccc gcatataacc ctccattccc
     4381 acgatgcaga ttttcgtcaa gactttgacc ggtaaaacca taacattgga agttgaatct
     4441 tccgatacca tcgacaacgt taagtcgaaa attcaagaca aggaaggtat ccctccagat
     4501 caacaaagat tgatctttgc cggtaagcag ctagaagacg gtagaacgct gtctgattac
     4561 aacattcaga aggagtccac cttacatctt gtgctaaggc taagaggtgg ttatcacgga
     4621 tccggagctt ggctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     4681 acgtcgtcca agtcgcaatt gacttacggt gctcgagcca gcaagcaccc caatcctctg
     4741 gcaaagagac tttttgagat tgccgaagca aagaagacaa acgttaccgt ctctgctgat
     4801 gtgacgacaa cccgagaact cctggacctc gctgaccgta cggaagctgt tggatccaat
     4861 acatatgccg tctagcaatg gactaatcaa cttttgatga tacaggtctc ggtccctaca
     4921 tcgccgtcat caagacacac atcgacatcc tcaccgattt cagcgtcgac actatcaatg
     4981 gcctgaatgt gctggctcaa aagcacaact ttttgatctt cgaggaccgc aaattcatcg
     5041 acatcggcaa taccgtccag aagcaatacc acggcggtgc tctgaggatc tccgaatggg
     5101 cccacattat caactgcagc gttctccctg gcgagggcat cgtcgaggct ctggcccaga
     5161 ccgcatctgc gcaagacttc ccctatggtc ctgagagagg actgttggtc ctggcagaga
     5221 tgacctccaa aggatcgctg gctacgggcg agtataccaa ggcatcggtt gactacgctc
     5281 gcaaatacaa gaacttcgtt atgggtttcg tgtcgacgcg ggccctgacg gaagtgcagt
     5341 cggatgtgtc ttcagcctcg gaggatgaag atttcgtggt cttcacgacg ggtgtgaacc
     5401 tctcttccaa aggagataag cttggacagc aataccagac tcctgcatcg gctattggac
     5461 gcggtgccga ctttatcatc gccggtcgag gcatctacgc tgctcccgac ccggttgaag
     5521 ctgcacagcg gtaccagaaa gaaggctggg aagcttatat ggccagagta tgcggcaagt
     5581 catgatttcc tcttggagca aaagtgtagt gccagtacga gtgttgtgga ggaaggctgc
     5641 atacattgtg cctgtcatta aacgatgagc tcgtccgtat tggcccctgt aatgccatgt
     5701 tttccgcccc caatcgtcaa ggttttccct ttgttagatt cctaccagtc atctagcaag
     5761 tgaggtaagc tttgccagaa acgccaaggc tttatctatg tagtcgataa gcaaagtgga
     5821 ctgatagctt aatatggaag gtccctcagg acaagtcgac ctgtgcagaa gagataacag
     5881 cttggcatca cgcatcagtg cctcctctca gacagaataa gtcgccataa gttatcgacc
     5941 gaacctaggt agggtatatt tggtgacgac aatacctccc gacgatacct ggattagaca
     6001 ccgtatgccg cgaattaacg ccttccgacc aagtcccttt tggtgatcga cactttagga
     6061 tcatctcctg ccagtcgccc agactgacgg tgttatggtg gcgcgctatg gctttcctag
     6121 ccggtcaagc tccgtgcact agagagcgga gaaggagggg gagttgatag ggaatgatat
     6181 ctaggtagag aagtactcca accagagata tgcggatcaa tgctccggca gttgttagca
     6241 taccccaacc tcgtttaagt atcctcttct tccatccatc tttatcatta tttgtctatg
     6301 tgcatcccta gatcacgtca attatttcga taaatggata cgtgcactcg ggaatgcgtg
     6361 attggctcgt ctcgtgttct cagttcctca ttccccccca cgttacattg acaacggagt
     6421 attagcctat ggtcctgttc gataagcttg ctccatcgcc tccactacgt caagcggagt
     6481 atcagacatg agctctaccc cagatatcgc ctgcttatcg ctggttgctt tgtcatgcaa
     6541 gtacagatca catcggcaat tctgataaca gatggtgcca ttgtcccgta atcaattgcc
     6601 cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6721 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6781 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6841 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6901 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6961 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7021 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7081 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7141 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7321 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8341 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8401 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8461 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8521 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8581 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8641 catgattacg ccactgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     8701 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     8761 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     8821 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     8881 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     8941 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     9001 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     9061 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     9121 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     9181 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     9241 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     9301 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     9361 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     9421 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     9481 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     9541 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     9601 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     9661 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     9721 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     9781 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     9841 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     9901 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     9961 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
    10021 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    10081 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10141 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10201 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataccacctt
    10261 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt
    10321 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
    10381 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
    10441 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
    10501 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
    10561 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
    10621 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
    10681 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
    10741 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
    10801 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
    10861 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
    10921 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
    10981 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
    11041 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
    11101 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
    11161 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
    11221 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
    11281 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
    11341 ttatatcagt tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    11401 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    11461 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    11521 tagaccgaga tagggttgag tggggtaccg agctcgaatt cactggccgt cgttttacaa
    11581 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    11641 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    11701 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    11761 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
    11821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
    11881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccag cttatttttt gtatactgtt
    11941 ttgtgatagc acgaagtttt tccacggtat cttgttaaaa atatatattt gtggcgggct
    12001 tacctacatc aaattaataa gagactaatt ataaactaaa cacacaagca agctacttta
    12061 gggtaaaagt ttataaatgc ttttgacgta taaacgttgc ttgtatttat tattacaatt
    12121 aaaggtggat agaaaaccta gagactagtt agaaactaat ctcaggtttg cgttaaacta
    12181 aatcagagcc cgagaggtta acagaaccta gaaggggact agatatccgg gtagggaaac
    12241 aaaaaaaaaa aacaagacag ccacatatta gggagactag ttagaagcta gttccaggac
    12301 taggaaaata aaagacaatg ataccacagt ctagttgaca actagataga ttctagattg
    12361 aggccaaagt ctctgagatc caggttagtt gcaactaata ctagttagta tctagtctcc
    12421 tataactctg aagctagaat aacttactac tattatcctc accactgttc agctgcgcaa
    12481 acggagtgat tgcaaggtgt tcagagacta gttattgact agtcagtgac tagcaataac
    12541 taacaaggta ttaacctacc atgtctgcca tcaccctgca cttcctcggg ctcagcagcc
    12601 ttttcctcct cattttcatg ctcattttcc ttgtttaaga ctgtgactag tcaaagacta
    12661 gtccagaacc acaaaggaga aatgtcttac cactttcttc attgcttgtc tcttttgcat
    12721 tatccatgtc tgcaactagt tagagtctag ttagtgacta gtccgacgag gacttgcttg
    12781 tctccggatt gttggaggaa ctctccaggg cctcaagatc cacaacagag ccttctagaa
    12841 gactggtcaa taactagttg gtctttgtct gagtctgact tacgaggttg catactcgct
    12901 ccctttgcct cgtcaatcga tgagaaaaag cgccaaaact cgcaatatgg ctttgaacca
    12961 cacggtgctg agactagtta gaatctagtc ccaaactagc ttggatagct tacctttgcc
    13021 ctttgcgttg cgacaggtct tgcagggtat ggttcctttc tcaccagctg atttagctgc
    13081 cttgctaccc tcacggcgga tctgcataaa gagtggctag aggttataaa ttagcactga
    13141 tcctaggtac ggggctgaat gtaacttgcc tttcctttct catcgcgcgg caagacaggc
    13201 ttgctcaaat tcctaccagt cacaggggta tgcacggcgt acggaccact tgaactagtc
    13261 acagattagt tagcaactag tctgcattga atggctgtac ttacgggccc tcgccattgt
    13321 cctgatcatt tccagcttca ccctcgttgc tgcaaagtag ttagtgacta gtcaaggact
    13381 agttgaaatg ggagaagaaa ctcacgaatt ctcgacaccc ttagtattgt ggtccttgga
    13441 cttggtgctg ctatatatta gctaatacac tagttagact cacagaaact tacgcagctc
    13501 gcttgcgctt cttggtagga gtcggggttg ggagaacagt gccttcaaac aagccttcat
    13561 accatgctac ttgactagtc agggactagt caccaagtaa tctagatagg acttgccttt
    13621 ggcctccatc agttccttca tagtgggagg tccattgtgc aatgtaaact ccatgccgtg
    13681 ggagttcttg tccttcaagt gcttgaccaa tatgtttctg ttggcagagg gaacctgtca
    13741 actagttaat aactagtcag aaactagtat agcagtagac tcactgtacg cttgaggcat
    13801 cccttcactc ggcagtagac ttcatatgga tggatatcag gcacgccatt gtcgtcctgt
    13861 ggactagtca gtaactaggc ttaaagctag tcgggtcggc ttactatctt gaaatccggc
    13921 agcgtaagct ccccgtcctt aactgcctcg agatagtgac agtactctgg ggactttcgg
    13981 agatcgttat cgcgaatgct cggcatacta atcgttgact agtcttggac tagtcccgag
    14041 caaaaaggat tggaggagga ggaggaaggt gagagtgaga caaagagcga aataagagct
    14101 tcaaaggcta tctctaagca gtatgaaggt taagtatcta gttcttgact agatttaaaa
    14161 gagatttcga ctagttatgt acctggagtt tggatatagg aatgtgttgt ggtaacgaaa
    14221 tgtaaggggg aggaaagaaa aagtcggtca agaggtaact ctaagtcggc cattcctttt
    14281 tgggaggcgc taaccataaa cggcatggtc gacttagagt tagctcaggg aatttaggga
    14341 gttatctgcg accactggac tctcaggctt tccattgggg tactaacata gccatcaaat
    14401 gccggcccgt gtttaatctc ctcgagatgg ctgggctcac atgcgttcta ctcaaaaact
    14461 cttataccta ggccctctcc caatcgtgta tgtttggacc ttcctctctc acactttgga
    14521 taacatcaag cata
//
