LOCUS       sequence-44531-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS5c-RFP
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(223..229,230..247,248..253))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(339..358)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        564..641
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(661..680)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cggacacctt tggtaacttt cagtttagcg gtctgggtac cttcgtacgg acgaccttca
       61 ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg aactttgaaa
      121 cgcatgaact ctttgataac gtcttcgcta ctcgccatat gtatatctcc ttcttaaaag
      181 atcttttgaa ttctgaaatt gttatccgct cacaattcca cacattatac gagccggaag
      241 cataaagtgt aaagcctggg gtgcctaaac gatctcgatc ctctacgccg gacgcatcgt
      301 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      361 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      421 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
      481 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
      541 taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
      601 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
      661 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
      721 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
      781 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
      841 ccacctccag tctggccctg cacgcgccgt
//
