LOCUS       sequence-44533-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-GCC
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..104
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggatctgcca ccatggctcc aagaagaagc gtaaggtaga ctacaaagac catgacggtg
       61 attataaaga tcatgacatc gattacaagg atgacgatga caagggtacc cgcccatatg
      121 cttgccctgt cgagtcctgt gaccgccgct tcagtgagcg gggaacactg gcccggcaca
      181 tccggattca cacaggccag aagcccttcc agtgtcgcat ctgcatgagg aacttcagcc
      241 agtctggtga cctcacccgc cacatccgca cccacacagg cgagaagcct tttgcatgcg
      301 acatctgcgg caggaagttc gcgcgcagcg acaacctctc tgagcacacc aagatccaca
      361 caggtggatc ccaactagtc aaaagtgaac tggaggagaa gaaatctgaa cttcgtcata
      421 aattgaaata tgtgcctcat gaatatattg aattaattga aattgccaga aattccactc
      481 aggatagaat tcttgaaatg aaggtaatgg aattttttat gaaagtttat ggatatagag
      541 gtaaacattt gggtggatca aggaaaccgg acggagcaat ttatactgtc ggatctccta
      601 ttgattacgg tgtgatcgtg gatactaaag cttatagcgg aggttataat ctgccaattg
      661 gccaagcaga tgaaatgcaa gactatgtcg aagaaaatca aacacgaaac aaacatatca
      721 accctaatga atggtggaaa gtctatccat cttctgtaac ggaatttaag tttttatttg
      781 tgagtggtca ctttaaagga aactacaaag ctcagcttac acgattaaat catatcacta
      841 attgtaatg
//
