LOCUS       sequence-44537-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0035
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atggcctctg gagccgattc aaaaggtgat
      121 gatttatcaa cagccattct caaacagaag aaccgaccca atcggttaat tgttgatgaa
      181 gccatcaatg aagataacag cgtggtgtcc ttgtcccagc ccaagatgga tgaactgcag
      241 ttgttccgag gtgacacggt gttgctaaaa ggaaagaaaa gacgggaagc tgtatgcatt
      301 gttctttctg atgacacgtg ttctgatgag aagattcgaa tgaatagagt tgttcggaat
      361 aacctccgag ttcgcctagg agatgtcatc agcatccagc catgccctga tgtaaagtat
      421 ggcaaacgta tccacgttct acccatcgat gacacagtgg aaggcatcac tggcaatctc
      481 tttgaggtat accttaagcc gtacttcctg gaagcttatc ggcccatccg taaaggagat
      541 atttttcttg tccggggtgg gatgcgtgct gtggagttca aagttgtaga gacagatccc
      601 agcccttact gtattgttgc tccagacaca gtgatccact gtgaggggga gccaatcaaa
      661 cgagaggatg aggaggaatc cttgaatgaa gtaggctatg atgacgtcgg tggttgcagg
      721 aagcagctag ctcagataaa ggagatggtg gagctgccac tgagacatcc tgcgctcttc
      781 aaggcgattg gtgtaaagcc tcctcgggga atcttgttgt at
//
