LOCUS       sequence-44542-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE11
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..57)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(79..228)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(253..273)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tactgggcca ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctctcc
       61 actgcctcta gagttaatca gcatgtccag gtcgaaatca tcaagggcgt cactgccgag
      121 catgtcgagg tcaaagtcat caagggcatc cgaaccaagc atgtcaaggt caaaatcatc
      181 gagggcgtca cttcccagca tatccagatc aaaatcgtcc aatgcgtcag cccgtccgga
      241 accgctggcc tccacctttc tcttcttctt ggggctagct gacgcgcgag tttggtcccc
      301 ttcatgcatg ggcgagggcg cgtccaaatc cctctccagc gagtctgcga acgcatgaag
      361 gctcgcctgg tcaggtgtct gagttgacgt aggtgaaggc ttcgcgcgtt tcataccgct
      421 cgcttggaga atcctgtccc accgctggga ggcgggaggc agtgtgcccg agcgggcctc
      481 gagttctgtg acaccgactc tacgaaagag ctgcagcagt ccgtgtctcg acataccaaa
      541 ttgagtcatg gcgtcatcga acgcttgtgc ggggtgggag tgacactgga agaatccgag
      601 cacgcggacc acttgcgcgt gatctgccac tcgatgtgat gtcctctcgg gaatccttct
      661 gttggtccgc ttaatcaatg caggcgcgtg cgggagcccc ttcttcaccg catccaaggc
      721 gggtcgtccg ccgaggcagg ccagcgctac aagatgatca ttagtgagtg cggccagcgc
      781 ggggtccggc ctcgaaagct gggccacgat tgactccagt gcgggtctgc cc
//
