LOCUS       sequence-44543-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: TALE12
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..68)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(90..239)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(264..284)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgcccttgc tcactgggcc aggattctcc tcgacgtcac cgcatgttag cagacttcct
       61 ctgccctctc cactgcctct agagttaatc agcatgtcca ggtcgaaatc atcaagggcg
      121 tcactgccga gcatgtcgag gtcaaagtca tcaagggcat ccgaaccaag catgtcaagg
      181 tcaaaatcat cgagggcgtc acttcccagc atatccagat caaaatcgtc caatgcgtca
      241 gcccgtccgg aaccgctggc ctccaccttt ctcttcttct tggggctagc tgacgcgcga
      301 gtttggtccc cttcatgcat gggcgagggc gcgtccaaat ccctctccag cgagtctgcg
      361 aacgcatgaa ggctcgcctg gtcaggtgtc tgagttgacg taggtgaagg cttcgcgcgt
      421 ttcataccgc tcgcttggag aatcctgtcc caccgctggg aggcgggagg cagtgtgccc
      481 gagcgggcct cgagttctgt gacaccgact ctacgaaaga gctgcagcag tccgtgtctc
      541 gacataccaa attgagtcat ggcgtcatcg aacgcttgtg cggggtggga gtgacactgg
      601 aagaatccga gcacgcggac cacttgcgcg tgatctgcca ctcgatgtga tgtcctctcg
      661 ggaatccttc tgttggtccg cttaatcaat gcaggcgcgt gcgggagccc cttcttcacc
      721 gcatccaagg cgggtcgtcc gccgaggcag gccagcgcta caagatgatc attagtgagt
      781 gcggccagcg cggggtccgg cctcgaaagc tgggccacga ttgactccag tgcgggtctg
      841 cccccgatat tggatgcaat agccactacc tgctcaggcg tgagtccgtg cgcttggcac
      901 agcacaggca acagcctctg tactgtttcc aaggcttgct tt
//
