LOCUS       sequence-445432-       15793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of heterologous genes under alcohol induction optimised 
            with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA 
            expression cassette; unique restriction sites: PacI, NotI, AsiSI, 
            AscI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Roux I, Woodcraft C, Sbaraini N, Pepper A, Wong E, Bracegirdle J, 
            Chooi YH
  TITLE     Next-generation AMA1-based plasmids for enhanced heterologous 
            expression in filamentous fungi.
  JOURNAL   Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 
            10.1111/1751-7915.70010.
  PUBMED    39276061
REFERENCE   2  (bases 1 to 15793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol."; date: "2024-09"; volume: "17(9):e70010. doi"; pages: "
            10.1111/1751-7915.70010"
COMMENT     Sequence Label: pYFAC-CH2-ubi-M-pyrG
FEATURES             Location/Qualifiers
     source          1..15793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1004..1231
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             join(3239..3307,3308..4099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3457..3476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4270..4858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4759..4778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5442..5475
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5554..6357)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6358..6578)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6749..6768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6790..12038
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     12208..12229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcccagaaaa ttacgggtat gtcctccact gtgttgaagt cagaaaaaca atcgataatt
       61 tgaagctcat taaaatgcca gttccgtcgt cgccgttgcc ctaggagcta ttccagtgct
      121 tagcttcatt catggatgta tcgtgactgg ccttcggaag gatgccaagg tcccctatcc
      181 gcacagctat gcgaccgttg agcaatgcaa gtcaaatgtg tgcatacctg gtctttggtt
      241 tggtacaccg ctatgtgaag attattaatg ctgaagtcat gatacaggcc aaagccgagc
      301 aatttaactg cgcgcagcgg gcccacgcta actacctcga gaatatgcct caaacaatgc
      361 tcttcaccct cttcgcgggt ctgaaatacc ctcacctggc aacagcaatt ggcgcttcat
      421 ggctgttttt ccgatctctc tacttgtacg gctatgtgta ctcgggtaag ccacaaggca
      481 agggcagatt gctgggaggt ttcttctggt tttctcaagg cgctctgtgg gctctgagtg
      541 tgtttggtgt tgccaaagac atgatctctt actgagagtt attctgtgtc tgacgaaata
      601 tgttgtgtat atatatatat gtacgttaaa agttccgtgg agttaccagt gattgaccaa
      661 tgttttatct tctacagttc tgcctgtcta ccccattcta gctgtacctg actacagagt
      721 agtttaattg tggttgaccc cacagtcgga ggcggaggaa tacagcaccg atgtggcctg
      781 tctccatcca gattggcacg caatttttac acgcggaaaa gatcgagata gagtacgact
      841 ttaaatttag tccccggcgg cttctatttt agaatatttg agatttgatt ctcaagcaat
      901 tgatttggtt gggtcaccct caattggata atatacctca ttgctcggct acttcaactc
      961 atcaatcacc gtcatacccc gcatataacc ctccattccc acgatgcaga ttttcgtcaa
     1021 gactttgacc ggtaaaacca taacattgga agttgaatct tccgatacca tcgacaacgt
     1081 taagtcgaaa attcaagaca aggaaggtat ccctccagat caacaaagat tgatctttgc
     1141 cggtaagcag ctagaagacg gtagaacgct gtctgattac aacattcaga aggagtccac
     1201 cttacatctt gtgctaaggc taagaggtgg tatgcacggg tccggagctt ggctgttgcc
     1261 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgtcgtcca agtcgcaatt
     1321 gacttacggt gctcgagcca gcaagcaccc caatcctctg gcaaagagac tttttgagat
     1381 tgccgaagca aagaagacaa acgttaccgt ctctgctgat gtgacgacaa cccgagaact
     1441 cctggacctc gctgaccgta cggaagctgt tggatccaat acatatgccg tctagcaatg
     1501 gactaatcaa cttttgatga tacaggtctc ggtccctaca tcgccgtcat caagacacac
     1561 atcgacatcc tcaccgattt cagcgtcgac actatcaatg gcctgaatgt gctggctcaa
     1621 aagcacaact ttttgatctt cgaggaccgc aaattcatcg acatcggcaa taccgtccag
     1681 aagcaatacc acggcggtgc tctgaggatc tccgaatggg cccacattat caactgcagc
     1741 gttctccctg gcgagggcat cgtcgaggct ctggcccaga ccgcatctgc gcaagacttc
     1801 ccctatggtc ctgagagagg actgttggtc ctggcagaga tgacctccaa aggatcgctg
     1861 gctacgggcg agtataccaa ggcatcggtt gactacgctc gcaaatacaa gaacttcgtt
     1921 atgggtttcg tgtcgacgcg ggccctgacg gaagtgcagt cggatgtgtc ttcagcctcg
     1981 gaggatgaag atttcgtggt cttcacgacg ggtgtgaacc tctcttccaa aggagataag
     2041 cttggacagc aataccagac tcctgcatcg gctattggac gcggtgccga ctttatcatc
     2101 gccggtcgag gcatctacgc tgctcccgac ccggttgaag ctgcacagcg gtaccagaaa
     2161 gaaggctggg aagcttatat ggccagagta tgcggcaagt catgatttcc tcttggagca
     2221 aaagtgtagt gccagtacga gtgttgtgga ggaaggctgc atacattgtg cctgtcatta
     2281 aacgatgagc tcgtccgtat tggcccctgt aatgccatgt tttccgcccc caatcgtcaa
     2341 ggttttccct ttgttagatt cctaccagtc atctagcaag tgaggtaagc tttgccagaa
     2401 acgccaaggc tttatctatg tagtcgataa gcaaagtgga ctgatagctt aatatggaag
     2461 gtccctcagg acaagtcgac ctgtgcagaa gagataacag cttggcatca cgcatcagtg
     2521 cctcctctca gacagaataa gtcgccataa gttatcgacc gaacctaggt agggtatatt
     2581 tggtgacgac aatacctccc gacgatacct ggattagaca ccgtatgccg cgaattaacg
     2641 ccttccgacc aagtcccttt tggtgatcga cactttagga tcatctcctg ccagtcgccc
     2701 agactgacgg tgttatggtg gcgcgctatg gctttcctag ccggtcaagc tccgtgcact
     2761 agagagcgga gaaggagggg gagttgatag ggaatgatat ctaggtagag aagtactcca
     2821 accagagata tgcggatcaa tgctccggca gttgttagca taccccaacc tcgtttaagt
     2881 atcctcttct tccatccatc tttatcatta tttgtctatg tgcatcccta gatcacgtca
     2941 attatttcga taaatggata cgtgcactcg ggaatgcgtg attggctcgt ctcgtgttct
     3001 cagttcctca ttccccccca cgttacattg acaacggagt attagcctat ggtcctgttc
     3061 gataagcttg ctccatcgcc tccactacgt caagcggagt atcagacatg agctctaccc
     3121 cagatatcgc ctgcttatcg ctggttgctt tgtcatgcaa gtacagatca catcggcaat
     3181 tctgataaca gatggtgcca ttgtcccgta atcaattgcc cattgaaaaa ggaagagtat
     3241 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3301 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3361 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3421 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3481 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3541 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3601 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3661 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3721 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3781 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3841 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3901 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3961 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4021 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4081 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4141 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4381 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcatgatta
     4921 cgaattcatc acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa
     4981 attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat
     5041 gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct
     5101 caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct
     5161 gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg
     5221 tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt
     5281 tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac
     5341 aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata
     5401 cgaggcgcgt gtaagttacg aattcgagct cggtacccgg gataacttcg tataatgtat
     5461 gctatacgaa gttatgggta ataactgata taattaaatt gaagctctaa tttgtgagtt
     5521 tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa
     5581 atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt
     5641 tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca catcatccac
     5701 ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat
     5761 aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat
     5821 aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga
     5881 tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac
     5941 ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt
     6001 cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt
     6061 attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa
     6121 tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt
     6181 ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt
     6241 acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt
     6301 ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat
     6361 ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg gttaagaata ctgggcaatt
     6421 tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct
     6481 tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca
     6541 aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa gcgtggtgca gcctgatgcg
     6601 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     6661 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6721 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6781 acgttcgcca gcttattttt tgtatactgt tttgtgatag cacgaagttt ttccacggta
     6841 tcttgttaaa aatatatatt tgtggcgggc ttacctacat caaattaata agagactaat
     6901 tataaactaa acacacaagc aagctacttt agggtaaaag tttataaatg cttttgacgt
     6961 ataaacgttg cttgtattta ttattacaat taaaggtgga tagaaaacct agagactagt
     7021 tagaaactaa tctcaggttt gcgttaaact aaatcagagc ccgagaggtt aacagaacct
     7081 agaaggggac tagatatccg ggtagggaaa caaaaaaaaa aaacaagaca gccacatatt
     7141 agggagacta gttagaagct agttccagga ctaggaaaat aaaagacaat gataccacag
     7201 tctagttgac aactagatag attctagatt gaggccaaag tctctgagat ccaggttagt
     7261 tgcaactaat actagttagt atctagtctc ctataactct gaagctagaa taacttacta
     7321 ctattatcct caccactgtt cagctgcgca aacggagtga ttgcaaggtg ttcagagact
     7381 agttattgac tagtcagtga ctagcaataa ctaacaaggt attaacctac catgtctgcc
     7441 atcaccctgc acttcctcgg gctcagcagc cttttcctcc tcattttcat gctcattttc
     7501 cttgtttaag actgtgacta gtcaaagact agtccagaac cacaaaggag aaatgtctta
     7561 ccactttctt cattgcttgt ctcttttgca ttatccatgt ctgcaactag ttagagtcta
     7621 gttagtgact agtccgacga ggacttgctt gtctccggat tgttggagga actctccagg
     7681 gcctcaagat ccacaacaga gccttctaga agactggtca ataactagtt ggtctttgtc
     7741 tgagtctgac ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg atgagaaaaa
     7801 gcgccaaaac tcgcaatatg gctttgaacc acacggtgct gagactagtt agaatctagt
     7861 cccaaactag cttggatagc ttacctttgc cctttgcgtt gcgacaggtc ttgcagggta
     7921 tggttccttt ctcaccagct gatttagctg ccttgctacc ctcacggcgg atctgcataa
     7981 agagtggcta gaggttataa attagcactg atcctaggta cggggctgaa tgtaacttgc
     8041 ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag tcacaggggt
     8101 atgcacggcg tacggaccac ttgaactagt cacagattag ttagcaacta gtctgcattg
     8161 aatggctgta cttacgggcc ctcgccattg tcctgatcat ttccagcttc accctcgttg
     8221 ctgcaaagta gttagtgact agtcaaggac tagttgaaat gggagaagaa actcacgaat
     8281 tctcgacacc cttagtattg tggtccttgg acttggtgct gctatatatt agctaataca
     8341 ctagttagac tcacagaaac ttacgcagct cgcttgcgct tcttggtagg agtcggggtt
     8401 gggagaacag tgccttcaaa caagccttca taccatgcta cttgactagt cagggactag
     8461 tcaccaagta atctagatag gacttgcctt tggcctccat cagttccttc atagtgggag
     8521 gtccattgtg caatgtaaac tccatgccgt gggagttctt gtccttcaag tgcttgacca
     8581 atatgtttct gttggcagag ggaacctgtc aactagttaa taactagtca gaaactagta
     8641 tagcagtaga ctcactgtac gcttgaggca tcccttcact cggcagtaga cttcatatgg
     8701 atggatatca ggcacgccat tgtcgtcctg tggactagtc agtaactagg cttaaagcta
     8761 gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc tccccgtcct taactgcctc
     8821 gagatagtga cagtactctg gggactttcg gagatcgtta tcgcgaatgc tcggcatact
     8881 aatcgttgac tagtcttgga ctagtcccga gcaaaaagga ttggaggagg aggaggaagg
     8941 tgagagtgag acaaagagcg aaataagagc ttcaaaggct atctctaagc agtatgaagg
     9001 ttaagtatct agttcttgac tagatttaaa agagatttcg actagttatg tacctggagt
     9061 ttggatatag gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa aaagtcggtc
     9121 aagaggtaac tctaagtcgg ccattccttt ttgggaggcg ctaaccataa acggcatggt
     9181 cgacttagag ttagctcagg gaatttaggg agttatctgc gaccactgga ctctcaggct
     9241 ttccattggg gtactaacat agccatcaaa tgccggcccg tgtttaatct cctcgagatg
     9301 gctgggctca catgcgttct actcaaaaac tcttatacct aggccctctc ccaatcgtgt
     9361 atgtttggac cttcctctct cacactttgg ataacatcaa gcatactgca gattttgacc
     9421 tagttaacaa ggcacatcat gctatcactt ccaacactct tgctgagcgc gttctgcctt
     9481 gcctttctgg ttctacgaag tatccagtcc cgcctgcaac gccgcagaaa cgccaaaagg
     9541 tggggttgtc aaccgccagc tagagctcca tcgggattct ttggcattcc gccgttcctc
     9601 ggtggtcgca gataactccc taaattccct gagctaactc taagtcgacc atgccgttta
     9661 tggttagcgc ctcccaaaaa ggaatggccg acttagagtt acctcttgac cgactttttc
     9721 tttcctcccc cttacatttc gttaccacaa cacattccta tatccaaact ccaggtacat
     9781 aactagtcga aatctctttt aaatctagtc aagaactaga tacttaacct tcatactgct
     9841 tagagatagc ctttgaagct cttatttcgc tctttgtctc actctcacct tcctcctcct
     9901 cctccaatcc tttttgctcg ggactagtcc aagactagtc aacgattagt atgccgagca
     9961 ttcgcgataa cgatctccga aagtccccag agtactgtca ctatctcgag gcagttaagg
    10021 acggggagct tacgctgccg gatttcaaga tagtaagccg acccgactag ctttaagcct
    10081 agttactgac tagtccacag gacgacaatg gcgtgcctga tatccatcca tatgaagtct
    10141 actgccgagt gaagggatgc ctcaagcgta cagtgagtct actgctatac tagtttctga
    10201 ctagttatta actagttgac aggttccctc tgccaacaga aacatattgg tcaagcactt
    10261 gaaggacaag aactcccacg gcatggagtt tacattgcac aatggacctc ccactatgaa
    10321 ggaactgatg gaggccaaag gcaagtccta tctagattac ttggtgacta gtccctgact
    10381 agtcaagtag catggtatga aggcttgttt gaaggcactg ttctcccaac cccgactcct
    10441 accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc taactagtgt attagctaat
    10501 atatagcagc accaagtcca aggaccacaa tactaagggt gtcgagaatt cgtgagtttc
    10561 ttctcccatt tcaactagtc cttgactagt cactaactac tttgcagcaa cgagggtgaa
    10621 gctggaaatg atcaggacaa tggcgagggc ccgtaagtac agccattcaa tgcagactag
    10681 ttgctaacta atctgtgact agttcaagtg gtccgtacgc cgtgcatacc cctgtgactg
    10741 gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa aggaaaggca agttacattc
    10801 agccccgtac ctaggatcag tgctaattta taacctctag ccactcttta tgcagatccg
    10861 ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa ggaaccatac cctgcaagac
    10921 ctgtcgcaac gcaaagggca aaggtaagct atccaagcta gtttgggact agattctaac
    10981 tagtctcagc accgtgtggt tcaaagccat attgcgagtt ttggcgcttt ttctcatcga
    11041 ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc agactcagac aaagaccaac
    11101 tagttattga ccagtcttct agaaggctct gttgtggatc ttgaggccct ggagagttcc
    11161 tccaacaatc cggagacaag caagtcctcg tcggactagt cactaactag actctaacta
    11221 gttgcagaca tggataatgc aaaagagaca agcaatgaag aaagtggtaa gacatttctc
    11281 ctttgtggtt ctggactagt ctttgactag tcacagtctt aaacaaggaa aatgagcatg
    11341 aaaatgagga ggaaaaggct gctgagcccg aggaagtgca gggtgatggc agacatggta
    11401 ggttaatacc ttgttagtta ttgctagtca ctgactagtc aataactagt ctctgaacac
    11461 cttgcaatca ctccgtttgc gcagctgaac agtggtgagg ataatagtag taagttattc
    11521 tagcttcaga gttataggag actagatact aactagtatt agttgcaact aacctggatc
    11581 tcagagactt tggcctcaat ctagaatcta tctagttgtc aactagactg tggtatcatt
    11641 gtcttttatt ttcctagtcc tggaactagc ttctaactag tctccctaat atgtggctgt
    11701 cttgtttttt ttttttgttt ccctacccgg atatctagtc cccttctagg ttctgttaac
    11761 ctctcgggct ctgatttagt ttaacgcaaa cctgagatta gtttctaact agtctctagg
    11821 ttttctatcc acctttaatt gtaataataa atacaagcaa cgtttatacg tcaaaagcat
    11881 ttataaactt ttaccctaaa gtagcttgct tgtgtgttta gtttataatt agtctcttat
    11941 taatttgatg taggtaagcc cgccacaaat atatattttt aacaagatac cgtggaaaaa
    12001 cttcgtgcta tcacaaaaca gtatacaaaa aataagctgg ctttccccgt caagctctaa
    12061 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12121 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12181 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12241 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
    12301 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaatg gggtgacgat
    12361 gagccgctct tgcatctttg tttgtattat actgtctttc ttgttacaca taattattct
    12421 agaatgcccc accgttacat acgggacaca gccatttaca tatgcatgtg gattacgagc
    12481 taacgagttc attcaaatct cagaactatc acataatcat cattccccta tcgtcaaaga
    12541 ccgtaagaca aatccggttc atgcactgaa cccattcggg tagtgagtca tttactcagc
    12601 acactcgcgc tgacgctcgt cgaacacctt caatgcctcc tcggcagcct tgacaccact
    12661 gagaaccatg gcaccgaagg tagggcccat gcggttaaag ccatcaattt cagacagctc
    12721 cataccgccg attatcaagc ccttagtaac ctcgcgggtg ttcttgacga tggcatcctg
    12781 cgctgattgt gatagttccc acttgtccgt ccgcatcggc atccgcagct cgggatagtt
    12841 ccgacctagg attggatgca tgcggaaccg cacgagggcg gggcggaaat tgacacacca
    12901 ctcctctcca cgcaccgttc aagaggtacg cgtatagagc cgtatagagc agagacggag
    12961 cactttctgg tactgtccgc acgggatgtc cgcacggaga gccacaaacg agcggggccc
    13021 cgtacgtgct ctcctacccc aggatcgcat ccccgcatag ctgaacatct atataaagac
    13081 ccccaaggtt ctcagtctca ccaacatcat caaccaacaa tcaacagttc tctactcagt
    13141 taattagaac tcttccaatc ctatcacctc gccttaatta agcggaacaa attgaagcga
    13201 gtccccgtgg agcgcgagcc cgcgagagag tcgtgcggga tgatgccatg aagtatcacc
    13261 tacattgtat gctctccaga tcgaagagga atactctgaa taagtggatt taggcggttt
    13321 ctttggtcct actatatgca cactcagatt ctgtcaattg aaaagtcagc ccgctgtcta
    13381 tcatgacttc tatctccatt aattgtcatc cagccgtgcc tctgtatggt gcgtataccc
    13441 tcgactcaca cctgtgaagt ctctgccact tagcgcagcc aaagaagatt tagtctaaag
    13501 gtctacaatc aattcaggcc gtattcaggg cggccgcttc tgctgtttct gggctcaatg
    13561 ctttggtatc tcaaaggaga ggagaagcgt ccgtggaagg caggaagacc tacaagacct
    13621 ggcactcaga aagagggcgg ttgctcttaa gtgtagggct gcgcagggga ggaactctgg
    13681 ggttgtcaaa tgcggcagac ggaaatctac attagcagcg tcaatccacc cccttttggt
    13741 gaaccataaa ggggctagtc taccccagct tcgaatcaga ataatataaa tgcggggaaa
    13801 gctttaggta tgcggacaga tccatactgg gtcctccaag agatcggccc gcacatctga
    13861 cgtggtttca aggttcgacc catgcggagc cgcattggag gatctatccc tgtaggtacc
    13921 ttagctaaag atctacgccg tatggccaag cggaagatat aggaacgatc ggccatgaag
    13981 tcgtgtcttc tacaaactct cgtatattca ctaggttcga cgtctgctaa actaacctga
    14041 cctaacctaa ccttaactcc ctgccagttc agcccatgcg gacccggcag tcatgtcttt
    14101 cgcttcagat tgaacgcggc tgcctcgtct cgatcaggat ttagcagaag acagtacaca
    14161 ctgaaaaagt accacaataa agaagctata ttgcaggcca aatagccgtc tgctcggtct
    14221 gtttattcaa cctggcctct ttcatcgttt ttcccttttc cttattaaac catatatctc
    14281 cagcagagcg atcgcgggag gagataattt agttgagatc gaagttggct ttatagttgt
    14341 aacagtgaaa tcagggcacg aatctttagt acttcgttga tcgccactcc accaagggtt
    14401 tgccattaag cataactggc aatatttttg tcaatttgcc ttttcgaccg tgtaaatgtc
    14461 cctatccata ctctccgcag tcaatatccg gaggtcaagt gtgaagactg tttaccccag
    14521 caagatactt ggacaagcct ggtttgcaat ccatagtttc agcaggcttc ctaggtggcc
    14581 ttaagcctgg gatactggct ggctgctagc aattgccatg actatgttgg aggaaatgtt
    14641 cgagaggaat cttgggtcca tgaacagagt agtcccactt caaatgttgc gtatgcctga
    14701 tattacggac caatagagga acaacctggc agcagctaac ttgtatatct ctaatcgcgt
    14761 tgacgaccgt tctgtgtcag tgcaactgcc acgcaaagac ttcagaataa taaactttgc
    14821 cagagaaggc agttgtatta tgttaatatc gcaacattgt gaaaattaaa tgaactaaca
    14881 atcaaaaaga aagaaaatcc gaactttccc tccgtattat ctaacccgtg tgcttttgca
    14941 acgcaatcca actattccag ttgacgacat taatccaagc cagcatagga agaataccaa
    15001 aaaccttcag acagccgtac ccgcaagcct tgttaccaac cagctatagc aatcttaccc
    15061 gttcaatttc ttaatcccca tctagacgct tccagcactc tgactgtcgg gagacgggtt
    15121 cctttgccag ccatgagcat tcgcctagtt agggagcggc ggatgcctac ttacttctgg
    15181 gaacgaggcg cgccgtgagc gggaaatatg ggtatcttgg gagaggagag ttggagtagg
    15241 gagaaatgct ggctcggtgg gaggacgagg gagctttaat aataccgctc tggctttggt
    15301 agcactgagc cagtggtcgc agcaaaagag gggaaaggag gggaacaggt caagcggagc
    15361 cgctcttgtt gaggatagct gttacccatg gaaattacat gtagcaagct agcaacccgc
    15421 cgaagtgccc cactggtctt caagagccag tccaggtgaa ctgcattccg tccccttcgc
    15481 cgcggtgccc actgcctcca cagcctagat gattctgaaa acacgttgaa gagctcgcga
    15541 taataaccga ggcgggtaat gcaggatgaa acttacgatc tacaacctac agcaccctgg
    15601 ttctgaaaca aagttgagga tccccgcgat cgataaccca gaccgtaatg cgaattgcaa
    15661 tttacgattt accccccgta gagcggactg attctggacc tatcttgaga ctatgtggtg
    15721 gagcctgcga tgataaccga ggccgtaatg caaaaatgaa aattacgaac caagaggcgt
    15781 gatgagaaga tca
//
