LOCUS       sequence-445434-       15792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of heterologous genes under alcohol induction optimised 
            with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA 
            expression cassette; unique restriction sites: PacI, NotI, AsiSI, 
            AscI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15792)
  AUTHORS   Roux I, Woodcraft C, Sbaraini N, Pepper A, Wong E, Bracegirdle J, 
            Chooi YH
  TITLE     Next-generation AMA1-based plasmids for enhanced heterologous 
            expression in filamentous fungi.
  JOURNAL   Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 
            10.1111/1751-7915.70010.
  PUBMED    39276061
REFERENCE   2  (bases 1 to 15792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol."; date: "2024-09"; volume: "17(9):e70010. doi"; pages: "
            10.1111/1751-7915.70010"
COMMENT     Sequence Label: pYFAC-CH2-ubi-Y-pyrG
FEATURES             Location/Qualifiers
     source          1..15792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1003..1230
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             join(3238..3306,3307..4098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3456..3475)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4269..4857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4758..4777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5441..5474
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5553..6356)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6357..6577)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6748..6767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6789..12037
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     12207..12228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcccagaaaa ttacgggtat gtcctccatg tgttgaagtc agaaaaacaa tcgataattt
       61 gaagctcatt aaaatgccag ttccgtcgtc gccgttgccc taggagctat tccagtgctt
      121 agcttcattc atggatgtat cgtgactggc cttcggaagg atgccaaggt cccctatccg
      181 cacagctatg cgaccgttga gcaatgcaag tcaaatgtgt gcatacctgg tctttggttt
      241 ggtacaccgc tatgtgaaga ttattaatgc tgaagtcatg atacaggcca aagccgagca
      301 atttaactgc gcgcagcggg cccacgctaa ctacctcgag aatatgcctc aaacaatgct
      361 cttcaccctc ttcgcgggtc tgaaataccc tcacctggca acagcaattg gcgcttcatg
      421 gctgtttttc cgatctctct acttgtacgg ctatgtgtac tcgggtaagc cacaaggcaa
      481 gggcagattg ctgggaggtt tcttctggtt ttctcaaggc gctctgtggg ctctgagtgt
      541 gtttggtgtt gccaaagaca tgatctctta ctgagagtta ttctgtgtct gacgaaatat
      601 gttgtgtata tatatatatg tacgttaaaa gttccgtgga gttaccagtg attgaccaat
      661 gttttatctt ctacagttct gcctgtctac cccattctag ctgtacctga ctacagagta
      721 gtttaattgt ggttgacccc acagtcggag gcggaggaat acagcaccga tgtggcctgt
      781 ctccatccag attggcacgc aatttttaca cgcggaaaag atcgagatag agtacgactt
      841 taaatttagt ccccggcggc ttctatttta gaatatttga gatttgattc tcaagcaatt
      901 gatttggttg ggtcaccctc aattggataa tatacctcat tgctcggcta cttcaactca
      961 tcaatcaccg tcataccccg catataaccc tccattccca cgatgcagat tttcgtcaag
     1021 actttgaccg gtaaaaccat aacattggaa gttgaatctt ccgataccat cgacaacgtt
     1081 aagtcgaaaa ttcaagacaa ggaaggtatc cctccagatc aacaaagatt gatctttgcc
     1141 ggtaagcagc tagaagacgg tagaacgctg tctgattaca acattcagaa ggagtccacc
     1201 ttacatcttg tgctaaggct aagaggtggt tatcacggat ccggagcttg gctgttgccc
     1261 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgtcgtccaa gtcgcaattg
     1321 acttacggtg ctcgagccag caagcacccc aatcctctgg caaagagact ttttgagatt
     1381 gccgaagcaa agaagacaaa cgttaccgtc tctgctgatg tgacgacaac ccgagaactc
     1441 ctggacctcg ctgaccgtac ggaagctgtt ggatccaata catatgccgt ctagcaatgg
     1501 actaatcaac ttttgatgat acaggtctcg gtccctacat cgccgtcatc aagacacaca
     1561 tcgacatcct caccgatttc agcgtcgaca ctatcaatgg cctgaatgtg ctggctcaaa
     1621 agcacaactt tttgatcttc gaggaccgca aattcatcga catcggcaat accgtccaga
     1681 agcaatacca cggcggtgct ctgaggatct ccgaatgggc ccacattatc aactgcagcg
     1741 ttctccctgg cgagggcatc gtcgaggctc tggcccagac cgcatctgcg caagacttcc
     1801 cctatggtcc tgagagagga ctgttggtcc tggcagagat gacctccaaa ggatcgctgg
     1861 ctacgggcga gtataccaag gcatcggttg actacgctcg caaatacaag aacttcgtta
     1921 tgggtttcgt gtcgacgcgg gccctgacgg aagtgcagtc ggatgtgtct tcagcctcgg
     1981 aggatgaaga tttcgtggtc ttcacgacgg gtgtgaacct ctcttccaaa ggagataagc
     2041 ttggacagca ataccagact cctgcatcgg ctattggacg cggtgccgac tttatcatcg
     2101 ccggtcgagg catctacgct gctcccgacc cggttgaagc tgcacagcgg taccagaaag
     2161 aaggctggga agcttatatg gccagagtat gcggcaagtc atgatttcct cttggagcaa
     2221 aagtgtagtg ccagtacgag tgttgtggag gaaggctgca tacattgtgc ctgtcattaa
     2281 acgatgagct cgtccgtatt ggcccctgta atgccatgtt ttccgccccc aatcgtcaag
     2341 gttttccctt tgttagattc ctaccagtca tctagcaagt gaggtaagct ttgccagaaa
     2401 cgccaaggct ttatctatgt agtcgataag caaagtggac tgatagctta atatggaagg
     2461 tccctcagga caagtcgacc tgtgcagaag agataacagc ttggcatcac gcatcagtgc
     2521 ctcctctcag acagaataag tcgccataag ttatcgaccg aacctaggta gggtatattt
     2581 ggtgacgaca atacctcccg acgatacctg gattagacac cgtatgccgc gaattaacgc
     2641 cttccgacca agtccctttt ggtgatcgac actttaggat catctcctgc cagtcgccca
     2701 gactgacggt gttatggtgg cgcgctatgg ctttcctagc cggtcaagct ccgtgcacta
     2761 gagagcggag aaggaggggg agttgatagg gaatgatatc taggtagaga agtactccaa
     2821 ccagagatat gcggatcaat gctccggcag ttgttagcat accccaacct cgtttaagta
     2881 tcctcttctt ccatccatct ttatcattat ttgtctatgt gcatccctag atcacgtcaa
     2941 ttatttcgat aaatggatac gtgcactcgg gaatgcgtga ttggctcgtc tcgtgttctc
     3001 agttcctcat tcccccccac gttacattga caacggagta ttagcctatg gtcctgttcg
     3061 ataagcttgc tccatcgcct ccactacgtc aagcggagta tcagacatga gctctacccc
     3121 agatatcgcc tgcttatcgc tggttgcttt gtcatgcaag tacagatcac atcggcaatt
     3181 ctgataacag atggtgccat tgtcccgtaa tcaattgccc attgaaaaag gaagagtatg
     3241 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3301 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3361 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3421 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3481 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3541 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3601 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3661 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3721 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3781 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3841 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3901 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3961 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4021 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4081 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4141 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4201 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4261 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4321 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4381 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4441 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4501 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4561 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4621 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4681 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4741 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4801 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4861 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcatgattac
     4921 gaattcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     4981 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     5041 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     5101 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     5161 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     5221 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
     5281 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
     5341 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
     5401 gaggcgcgtg taagttacga attcgagctc ggtacccggg ataacttcgt ataatgtatg
     5461 ctatacgaag ttatgggtaa taactgatat aattaaattg aagctctaat ttgtgagttt
     5521 agtatacatg catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa
     5581 tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt
     5641 gcaaatagtc ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg
     5701 gttctatact gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata
     5761 atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata
     5821 acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat
     5881 agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact
     5941 tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc
     6001 gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta
     6061 ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat
     6121 gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt
     6181 tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta
     6241 cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg
     6301 gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt
     6361 tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt
     6421 catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt
     6481 cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa
     6541 aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag cgtggtgcag cctgatgcgg
     6601 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     6661 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6721 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6781 cgttcgccag cttatttttt gtatactgtt ttgtgatagc acgaagtttt tccacggtat
     6841 cttgttaaaa atatatattt gtggcgggct tacctacatc aaattaataa gagactaatt
     6901 ataaactaaa cacacaagca agctacttta gggtaaaagt ttataaatgc ttttgacgta
     6961 taaacgttgc ttgtatttat tattacaatt aaaggtggat agaaaaccta gagactagtt
     7021 agaaactaat ctcaggtttg cgttaaacta aatcagagcc cgagaggtta acagaaccta
     7081 gaaggggact agatatccgg gtagggaaac aaaaaaaaaa aacaagacag ccacatatta
     7141 gggagactag ttagaagcta gttccaggac taggaaaata aaagacaatg ataccacagt
     7201 ctagttgaca actagataga ttctagattg aggccaaagt ctctgagatc caggttagtt
     7261 gcaactaata ctagttagta tctagtctcc tataactctg aagctagaat aacttactac
     7321 tattatcctc accactgttc agctgcgcaa acggagtgat tgcaaggtgt tcagagacta
     7381 gttattgact agtcagtgac tagcaataac taacaaggta ttaacctacc atgtctgcca
     7441 tcaccctgca cttcctcggg ctcagcagcc ttttcctcct cattttcatg ctcattttcc
     7501 ttgtttaaga ctgtgactag tcaaagacta gtccagaacc acaaaggaga aatgtcttac
     7561 cactttcttc attgcttgtc tcttttgcat tatccatgtc tgcaactagt tagagtctag
     7621 ttagtgacta gtccgacgag gacttgcttg tctccggatt gttggaggaa ctctccaggg
     7681 cctcaagatc cacaacagag ccttctagaa gactggtcaa taactagttg gtctttgtct
     7741 gagtctgact tacgaggttg catactcgct ccctttgcct cgtcaatcga tgagaaaaag
     7801 cgccaaaact cgcaatatgg ctttgaacca cacggtgctg agactagtta gaatctagtc
     7861 ccaaactagc ttggatagct tacctttgcc ctttgcgttg cgacaggtct tgcagggtat
     7921 ggttcctttc tcaccagctg atttagctgc cttgctaccc tcacggcgga tctgcataaa
     7981 gagtggctag aggttataaa ttagcactga tcctaggtac ggggctgaat gtaacttgcc
     8041 tttcctttct catcgcgcgg caagacaggc ttgctcaaat tcctaccagt cacaggggta
     8101 tgcacggcgt acggaccact tgaactagtc acagattagt tagcaactag tctgcattga
     8161 atggctgtac ttacgggccc tcgccattgt cctgatcatt tccagcttca ccctcgttgc
     8221 tgcaaagtag ttagtgacta gtcaaggact agttgaaatg ggagaagaaa ctcacgaatt
     8281 ctcgacaccc ttagtattgt ggtccttgga cttggtgctg ctatatatta gctaatacac
     8341 tagttagact cacagaaact tacgcagctc gcttgcgctt cttggtagga gtcggggttg
     8401 ggagaacagt gccttcaaac aagccttcat accatgctac ttgactagtc agggactagt
     8461 caccaagtaa tctagatagg acttgccttt ggcctccatc agttccttca tagtgggagg
     8521 tccattgtgc aatgtaaact ccatgccgtg ggagttcttg tccttcaagt gcttgaccaa
     8581 tatgtttctg ttggcagagg gaacctgtca actagttaat aactagtcag aaactagtat
     8641 agcagtagac tcactgtacg cttgaggcat cccttcactc ggcagtagac ttcatatgga
     8701 tggatatcag gcacgccatt gtcgtcctgt ggactagtca gtaactaggc ttaaagctag
     8761 tcgggtcggc ttactatctt gaaatccggc agcgtaagct ccccgtcctt aactgcctcg
     8821 agatagtgac agtactctgg ggactttcgg agatcgttat cgcgaatgct cggcatacta
     8881 atcgttgact agtcttggac tagtcccgag caaaaaggat tggaggagga ggaggaaggt
     8941 gagagtgaga caaagagcga aataagagct tcaaaggcta tctctaagca gtatgaaggt
     9001 taagtatcta gttcttgact agatttaaaa gagatttcga ctagttatgt acctggagtt
     9061 tggatatagg aatgtgttgt ggtaacgaaa tgtaaggggg aggaaagaaa aagtcggtca
     9121 agaggtaact ctaagtcggc cattcctttt tgggaggcgc taaccataaa cggcatggtc
     9181 gacttagagt tagctcaggg aatttaggga gttatctgcg accactggac tctcaggctt
     9241 tccattgggg tactaacata gccatcaaat gccggcccgt gtttaatctc ctcgagatgg
     9301 ctgggctcac atgcgttcta ctcaaaaact cttataccta ggccctctcc caatcgtgta
     9361 tgtttggacc ttcctctctc acactttgga taacatcaag catactgcag attttgacct
     9421 agttaacaag gcacatcatg ctatcacttc caacactctt gctgagcgcg ttctgccttg
     9481 cctttctggt tctacgaagt atccagtccc gcctgcaacg ccgcagaaac gccaaaaggt
     9541 ggggttgtca accgccagct agagctccat cgggattctt tggcattccg ccgttcctcg
     9601 gtggtcgcag ataactccct aaattccctg agctaactct aagtcgacca tgccgtttat
     9661 ggttagcgcc tcccaaaaag gaatggccga cttagagtta cctcttgacc gactttttct
     9721 ttcctccccc ttacatttcg ttaccacaac acattcctat atccaaactc caggtacata
     9781 actagtcgaa atctctttta aatctagtca agaactagat acttaacctt catactgctt
     9841 agagatagcc tttgaagctc ttatttcgct ctttgtctca ctctcacctt cctcctcctc
     9901 ctccaatcct ttttgctcgg gactagtcca agactagtca acgattagta tgccgagcat
     9961 tcgcgataac gatctccgaa agtccccaga gtactgtcac tatctcgagg cagttaagga
    10021 cggggagctt acgctgccgg atttcaagat agtaagccga cccgactagc tttaagccta
    10081 gttactgact agtccacagg acgacaatgg cgtgcctgat atccatccat atgaagtcta
    10141 ctgccgagtg aagggatgcc tcaagcgtac agtgagtcta ctgctatact agtttctgac
    10201 tagttattaa ctagttgaca ggttccctct gccaacagaa acatattggt caagcacttg
    10261 aaggacaaga actcccacgg catggagttt acattgcaca atggacctcc cactatgaag
    10321 gaactgatgg aggccaaagg caagtcctat ctagattact tggtgactag tccctgacta
    10381 gtcaagtagc atggtatgaa ggcttgtttg aaggcactgt tctcccaacc ccgactccta
    10441 ccaagaagcg caagcgagct gcgtaagttt ctgtgagtct aactagtgta ttagctaata
    10501 tatagcagca ccaagtccaa ggaccacaat actaagggtg tcgagaattc gtgagtttct
    10561 tctcccattt caactagtcc ttgactagtc actaactact ttgcagcaac gagggtgaag
    10621 ctggaaatga tcaggacaat ggcgagggcc cgtaagtaca gccattcaat gcagactagt
    10681 tgctaactaa tctgtgacta gttcaagtgg tccgtacgcc gtgcataccc ctgtgactgg
    10741 taggaatttg agcaagcctg tcttgccgcg cgatgagaaa ggaaaggcaa gttacattca
    10801 gccccgtacc taggatcagt gctaatttat aacctctagc cactctttat gcagatccgc
    10861 cgtgagggta gcaaggcagc taaatcagct ggtgagaaag gaaccatacc ctgcaagacc
    10921 tgtcgcaacg caaagggcaa aggtaagcta tccaagctag tttgggacta gattctaact
    10981 agtctcagca ccgtgtggtt caaagccata ttgcgagttt tggcgctttt tctcatcgat
    11041 tgacgaggca aagggagcga gtatgcaacc tcgtaagtca gactcagaca aagaccaact
    11101 agttattgac cagtcttcta gaaggctctg ttgtggatct tgaggccctg gagagttcct
    11161 ccaacaatcc ggagacaagc aagtcctcgt cggactagtc actaactaga ctctaactag
    11221 ttgcagacat ggataatgca aaagagacaa gcaatgaaga aagtggtaag acatttctcc
    11281 tttgtggttc tggactagtc tttgactagt cacagtctta aacaaggaaa atgagcatga
    11341 aaatgaggag gaaaaggctg ctgagcccga ggaagtgcag ggtgatggca gacatggtag
    11401 gttaatacct tgttagttat tgctagtcac tgactagtca ataactagtc tctgaacacc
    11461 ttgcaatcac tccgtttgcg cagctgaaca gtggtgagga taatagtagt aagttattct
    11521 agcttcagag ttataggaga ctagatacta actagtatta gttgcaacta acctggatct
    11581 cagagacttt ggcctcaatc tagaatctat ctagttgtca actagactgt ggtatcattg
    11641 tcttttattt tcctagtcct ggaactagct tctaactagt ctccctaata tgtggctgtc
    11701 ttgttttttt tttttgtttc cctacccgga tatctagtcc ccttctaggt tctgttaacc
    11761 tctcgggctc tgatttagtt taacgcaaac ctgagattag tttctaacta gtctctaggt
    11821 tttctatcca cctttaattg taataataaa tacaagcaac gtttatacgt caaaagcatt
    11881 tataaacttt taccctaaag tagcttgctt gtgtgtttag tttataatta gtctcttatt
    11941 aatttgatgt aggtaagccc gccacaaata tatattttta acaagatacc gtggaaaaac
    12001 ttcgtgctat cacaaaacag tatacaaaaa ataagctggc tttccccgtc aagctctaaa
    12061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    12121 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    12181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    12241 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
    12301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatgg ggtgacgatg
    12361 agccgctctt gcatctttgt ttgtattata ctgtctttct tgttacacat aattattcta
    12421 gaatgcccca ccgttacata cgggacacag ccatttacat atgcatgtgg attacgagct
    12481 aacgagttca ttcaaatctc agaactatca cataatcatc attcccctat cgtcaaagac
    12541 cgtaagacaa atccggttca tgcactgaac ccattcgggt agtgagtcat ttactcagca
    12601 cactcgcgct gacgctcgtc gaacaccttc aatgcctcct cggcagcctt gacaccactg
    12661 agaaccatgg caccgaaggt agggcccatg cggttaaagc catcaatttc agacagctcc
    12721 ataccgccga ttatcaagcc cttagtaacc tcgcgggtgt tcttgacgat ggcatcctgc
    12781 gctgattgtg atagttccca cttgtccgtc cgcatcggca tccgcagctc gggatagttc
    12841 cgacctagga ttggatgcat gcggaaccgc acgagggcgg ggcggaaatt gacacaccac
    12901 tcctctccac gcaccgttca agaggtacgc gtatagagcc gtatagagca gagacggagc
    12961 actttctggt actgtccgca cgggatgtcc gcacggagag ccacaaacga gcggggcccc
    13021 gtacgtgctc tcctacccca ggatcgcatc cccgcatagc tgaacatcta tataaagacc
    13081 cccaaggttc tcagtctcac caacatcatc aaccaacaat caacagttct ctactcagtt
    13141 aattagaact cttccaatcc tatcacctcg ccttaattaa gcggaacaaa ttgaagcgag
    13201 tccccgtgga gcgcgagccc gcgagagagt cgtgcgggat gatgccatga agtatcacct
    13261 acattgtatg ctctccagat cgaagaggaa tactctgaat aagtggattt aggcggtttc
    13321 tttggtccta ctatatgcac actcagattc tgtcaattga aaagtcagcc cgctgtctat
    13381 catgacttct atctccatta attgtcatcc agccgtgcct ctgtatggtg cgtataccct
    13441 cgactcacac ctgtgaagtc tctgccactt agcgcagcca aagaagattt agtctaaagg
    13501 tctacaatca attcaggccg tattcagggc ggccgcttct gctgtttctg ggctcaatgc
    13561 tttggtatct caaaggagag gagaagcgtc cgtggaaggc aggaagacct acaagacctg
    13621 gcactcagaa agagggcggt tgctcttaag tgtagggctg cgcaggggag gaactctggg
    13681 gttgtcaaat gcggcagacg gaaatctaca ttagcagcgt caatccaccc ccttttggtg
    13741 aaccataaag gggctagtct accccagctt cgaatcagaa taatataaat gcggggaaag
    13801 ctttaggtat gcggacagat ccatactggg tcctccaaga gatcggcccg cacatctgac
    13861 gtggtttcaa ggttcgaccc atgcggagcc gcattggagg atctatccct gtaggtacct
    13921 tagctaaaga tctacgccgt atggccaagc ggaagatata ggaacgatcg gccatgaagt
    13981 cgtgtcttct acaaactctc gtatattcac taggttcgac gtctgctaaa ctaacctgac
    14041 ctaacctaac cttaactccc tgccagttca gcccatgcgg acccggcagt catgtctttc
    14101 gcttcagatt gaacgcggct gcctcgtctc gatcaggatt tagcagaaga cagtacacac
    14161 tgaaaaagta ccacaataaa gaagctatat tgcaggccaa atagccgtct gctcggtctg
    14221 tttattcaac ctggcctctt tcatcgtttt tcccttttcc ttattaaacc atatatctcc
    14281 agcagagcga tcgcgggagg agataattta gttgagatcg aagttggctt tatagttgta
    14341 acagtgaaat cagggcacga atctttagta cttcgttgat cgccactcca ccaagggttt
    14401 gccattaagc ataactggca atatttttgt caatttgcct tttcgaccgt gtaaatgtcc
    14461 ctatccatac tctccgcagt caatatccgg aggtcaagtg tgaagactgt ttaccccagc
    14521 aagatacttg gacaagcctg gtttgcaatc catagtttca gcaggcttcc taggtggcct
    14581 taagcctggg atactggctg gctgctagca attgccatga ctatgttgga ggaaatgttc
    14641 gagaggaatc ttgggtccat gaacagagta gtcccacttc aaatgttgcg tatgcctgat
    14701 attacggacc aatagaggaa caacctggca gcagctaact tgtatatctc taatcgcgtt
    14761 gacgaccgtt ctgtgtcagt gcaactgcca cgcaaagact tcagaataat aaactttgcc
    14821 agagaaggca gttgtattat gttaatatcg caacattgtg aaaattaaat gaactaacaa
    14881 tcaaaaagaa agaaaatccg aactttccct ccgtattatc taacccgtgt gcttttgcaa
    14941 cgcaatccaa ctattccagt tgacgacatt aatccaagcc agcataggaa gaataccaaa
    15001 aaccttcaga cagccgtacc cgcaagcctt gttaccaacc agctatagca atcttacccg
    15061 ttcaatttct taatccccat ctagacgctt ccagcactct gactgtcggg agacgggttc
    15121 ctttgccagc catgagcatt cgcctagtta gggagcggcg gatgcctact tacttctggg
    15181 aacgaggcgc gccgtgagcg ggaaatatgg gtatcttggg agaggagagt tggagtaggg
    15241 agaaatgctg gctcggtggg aggacgaggg agctttaata ataccgctct ggctttggta
    15301 gcactgagcc agtggtcgca gcaaaagagg ggaaaggagg ggaacaggtc aagcggagcc
    15361 gctcttgttg aggatagctg ttacccatgg aaattacatg tagcaagcta gcaacccgcc
    15421 gaagtgcccc actggtcttc aagagccagt ccaggtgaac tgcattccgt ccccttcgcc
    15481 gcggtgccca ctgcctccac agcctagatg attctgaaaa cacgttgaag agctcgcgat
    15541 aataaccgag gcgggtaatg caggatgaaa cttacgatct acaacctaca gcaccctggt
    15601 tctgaaacaa agttgaggat ccccgcgatc gataacccag accgtaatgc gaattgcaat
    15661 ttacgattta ccccccgtag agcggactga ttctggacct atcttgagac tatgtggtgg
    15721 agcctgcgat gataaccgag gccgtaatgc aaaaatgaaa attacgaacc aagaggcgtg
    15781 atgagaagat ca
//
