LOCUS       sequence-445439-        7768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLS
FEATURES             Location/Qualifiers
     source          1..7768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..142
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1007..1046
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1077..1665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1566..1585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1819..1836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1851..1990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1916..1935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2076..2098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2092..2283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2520..2539
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2520..2538
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4927..4944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(7691..7709)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7705..7752
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tatccgacta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
       61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga
      241 cgcagaggac caattggggg cacgagtggg atacatagaa ctggacttga atagcggtaa
      301 aatccttgag agttttcgcc ctgaagagcg ttttccaatg atgagcactt tcaaagttct
      361 gctatgtgga gcagtattat cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat
      421 acactattcg cagaatgact tggttgaata ctccccagtg acagaaaagc accttacgga
      481 cggaatgacg gtaagagaat tatgtagtgc cgccataacg atgagtgata acactgcggc
      541 gaacttactt ctgacaacca tcggtggacc gaaggaatta accgcttttt tgcacaatat
      601 gggagaccat gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa
      661 cgacgagcga gacaccacaa tgcctgcggc aatggcaaca acattacgca aactattaac
      721 tggcgaacta cttactctgg cttcacggca acaattaata gactggcttg aagcggataa
      781 agttgcagga ccactactgc gttcggcact tcctgctggc tggtttattg ctgataaatc
      841 tggggcagga gagcgtggtt cacggggtat cattgccgca cttggaccag atggtaagcc
      901 ttcccgtatc gtagttatct acacgacggg tagtcaggca actatggacg aacgaaatag
      961 acagattgct gaaatagggg cttcactgat taagcattgg taaaccgata caattaaagg
     1021 ctccttttgg agcctttttt tttggacgga ccggtagaaa agatcaaagg atcttcttga
     1081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1201 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1741 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1801 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     1861 tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa
     1921 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     1981 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     2041 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     2101 ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg
     2161 aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag
     2221 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt
     2281 cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacga
     2341 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2401 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     2461 tgccagcgct tcgttaatac agatgtaggt gttggcacca tgcatcgatc ccgcgaaatt
     2521 aatacgactc actataggga gaccacaacg gtttccctct agagatagct tagcgatgtg
     2581 aaagaagctg gagaaaactt tcaagaaaaa gtttcttctt cagacgataa agagaagtca
     2641 aaaaataagc ggaaaataaa aggcgtaaat cagattaaaa aagcaacgga tattaaaagc
     2701 tctactaaga ttaaaagttc taatgatatt aaatcatcta cagatttaaa aacaatggga
     2761 tcataagcga aaggagaaat gagatatgtc tattcaaaaa agtactaata gttcaagttt
     2821 agcagaagtc attgaccgta ttttagataa aggaattgtt attgatgctt ttgcaagagt
     2881 ttctgttgta ggaattgaaa ttttaacgat tgaagcgcga gtggttattg ccagtgttga
     2941 tacatggtta cgctatgcag aagcagtagg gcttcttcgt gacgacgtag aagaaaacgg
     3001 tcttcctgaa cgttcaaatt caagtgaagg gcagccgcgt tttagtattt aatatgaagt
     3061 ggtatggaac cttccttttt tgagggtggt tccagctttt agatctaact attggaggct
     3121 actaaaaatg gaaattaaaa aaattatgca agccgtgaac gactttttcg gtgaacacgt
     3181 agctcctcct cataaaatta cctcggtgga agctactgaa gatgaaggtt ggagagttat
     3241 tgttgaagtc attgaagaac gagaatatat gaaaaaatac gccaaagatg aaatgctcgg
     3301 aacgtacgag tgctttgtaa ataaagaaaa agaagtcatt tcattcaaac gactcgacgt
     3361 cagatataga agcgccattg gcattgaagc ataaacgata agatggcagg aggaacgtaa
     3421 aaatgaccgt cttaacagac aaaaggaaaa aaggcagtgg agcttttata caagatgacg
     3481 agacaaaaga ggttctttca agagcgctga gctatttaaa atccggctat tccattcatt
     3541 ttacaggtcc tgccggcgga ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa
     3601 agcgtcctgt aatgctgatg cacgggaatc acgagctcaa caacaaagat ttaattggcg
     3661 attttacggg atacacgagc aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa
     3721 aagatgaaca ggtgagtgaa aactggcagg atggccgatt gcttgaagct gtaaaaaatg
     3781 gctatacgct gatttacgac gaatttactc gttctaagcc tgcgacgaat aatatctttc
     3841 tatcgatatt agaagaaggc gtgctgccgc tgtatggagt aaaaatgacc gatccttttg
     3901 tgcgcgtgca tcccgatttc cgcgtcatct tcacaagcaa tccagctgag tatgccggcg
     3961 tatatgatac gcaagatgcg cttctcgaca ggttaattac catgtttatt gattataaag
     4021 acatcgacag agagacagcg attttaacgg agaaaacgga cgtagaagaa gatgaagcgc
     4081 gcacaattgt aacgctcgta gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg
     4141 gacttagcct gcgggcttcg cttatgatcg ctacccttgc cacgcagcaa gacattccta
     4201 tcgatggaag tgacgaagat tttcaaacgt tatgtatcga tattttgcat catccgctta
     4261 ccaaatgttt ggatgaagaa aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat
     4321 gtaagaatat agacactgaa gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga
     4381 aacaggtatt tatattttta gcgccattca aacggataaa gacgaagaat ttggcgccgt
     4441 ggaagtagaa ggaacaaaag ctgaaacatt tttgattcgc tacaaagacg cggctatggt
     4501 agcagctgaa gtaccgatga aaatttatca tcctaatcgc caaaatttat taatgcatca
     4561 aaacgcagta gcagcgatta tggacaagaa cgatacggtt attccaatca gctttgggaa
     4621 tgtattcaaa tcaaaagaag acgtaaaagt tcttttggaa aacctttatc cgcagtttga
     4681 aaagctgttt ccagcgatca aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa
     4741 agaatggctt gagaaaaaag taaacgaaaa tcctgaactt gagaaagtat cagcatccgt
     4801 aaaaggaaaa tcagaagcag ccggttatta tgagcgtatt caacttggag gaatggctca
     4861 aaagatgttt acttccctgc aaaaagaagt caagacagat gtgttttctc cgcttgaaga
     4921 agcagcggaa gcagcaaaag caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc
     4981 tttcttaatt aaccgagaag atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga
     5041 aaactggaaa gacaaagccg attttcatta cagcggtcct tggcctgctt ataattttgt
     5101 gaacattcgc ctaaaagtag aagagaaata acgtgcttca caaattagta accgcaccca
     5161 ttaaccttgt agtgaaaatc ggcgaaaaag tacaggaaga agctgataaa cagctatatg
     5221 accttccgac gattcagcaa aagctcattc agcttcaaat gatgtttgag cttggtgaaa
     5281 ttccagaaga agcgtttcaa gaaaaagaag atgaattgtt aatgaggtac gaaattgcga
     5341 aacgcagaga aattgaacaa tgggaagggc taacacaaaa aagaaatgag gaatcctaga
     5401 cccttgcgag cagtcgtatg ctttctcgaa ttccgagcgg ttaagcgtga cagtccagga
     5461 ggaattaata gatgcaaccg gtcagccaag caaatggacg aatccacttg gatcctgatc
     5521 aagctgaaca aggcttagcg cagcttgtga tgacagttat tgagctattg aggcaaatcg
     5581 ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt gacggacgaa caaattgaaa
     5641 acttaggaat tgcactaatg aacttagaag aaaaaatgga cgagttgaaa gaggtgttcg
     5701 gtctggatgc agaagattta aatattgatc ttggcccgct aggcagcctg ctttaagcgg
     5761 tcagtaggag gaacagtatg gcagtcgaac ataatatgca gtcaagtacg attgtagatg
     5821 tgctcgaaaa gattttggat aaaggagtcg ttatagcggg ggacatcacc gtaggaattg
     5881 cagatgtcga gctattaacg ataaagatcc gcttgattgt ggcttcggtt gataaggcaa
     5941 aagaaatcgg catggactgg tgggaaaatg atccgtatct cagttcaaaa ggagccaata
     6001 acaaagcgct cgaagaagaa aataaaatgc tgcatgagcg gttaaaaacg cttgaagaaa
     6061 aaatagaaac gaaacgttaa aaactgtacg ctacttaaaa aatggaggga tttacaatgg
     6121 caactgaaac aaaattagat aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa
     6181 acggttcaaa agaaaagaac ggttcaaaag caagcaaaac aacaagcagc gggccaatca
     6241 aacgagcggt agcaggaggc atcatcggtg caacgattgg atatgtatcg actcctgaaa
     6301 atcgaaaaag tctccttgac cgcattgata cagacgaatt aaaaagcaaa gcatctgatt
     6361 taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg
     6421 gaagcttgtt taaaaaagat aaagataaat caaaagatga tgaagaaaac gtaaattctt
     6481 ctagtagcga aacagaagac gataacgttc aagagtacga cgagttaaaa gaagaaaatc
     6541 aaactcttca agatcgctta tcacagcttg aagaaaaaat gaacatgctt gttgagctta
     6601 gcctcaataa aaatcaagac gaagaagcgg aagatacaga ttccgacgaa gaagagaacg
     6661 atgagaacga tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa gaaacatcta
     6721 agccacgtaa aaaggataaa aaagaagctg aggaagaaga aagtgaaagt gacgaagaca
     6781 gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag
     6841 aagaagacga agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag
     6901 ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa
     6961 catctctttc tagtgaagac gatacaacag cctaagacgt aaaggaggaa agaaagacat
     7021 gagtacaggc ccttcttttt caactaaaga caatacgctt gaatactttg tgaaagcttc
     7081 taataaacac ggcttttcac ttgatatttc attaaatgta aacggcgctg tgatttccgg
     7141 taccatgatt tcagcaaaag aatactttga ttacttaagc gaaacgtttg aagaaggcag
     7201 tgaagtggct caggcgctaa gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa
     7261 cggagaagca gaagcccatt ttattcattt gaaaaataca aagatttact gtggagacag
     7321 taaatctact ccttctaaag gcaaaatctt ttggagaggg aaaatagcag aagtagacgg
     7381 gtttttctta ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt cataaaaaac
     7441 ggcggggtga ttgccccgcc gttttttagt gatgtgatga gatgtgcagc ttcttttttc
     7501 gatacatgag cggagtcatc ttcatttctt ttcgaaactg attaataaaa tagcttgtac
     7561 tattaaaccc tacttgatag gcgacttccg tcacatttgc ttctgcttgc tgcagtccgg
     7621 attcgcgaac gcgtctcgag agatccggct gctaacaaag cccgaaagga agctgagttg
     7681 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     7741 aggggttttt tgctgaaagg aggaacta
//
