LOCUS       sequence-445440-        7769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLK
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6027..6045)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6041..6088
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6155..6246
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7111..7150
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7181..7769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7670..7689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagagtgag gaattaacat tatgtctctt aaacaatcca tggagaataa
     3781 agatattgct cttattgata ttttagatgt cattttagat aaaggagtcg ccattaaagg
     3841 agacttaatc atttccatag ctggcgtcga tttagtgtat ttggatttgc gggtgcttat
     3901 ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat cacaaaccaa tcacttctga
     3961 acaatttgat aaacaaaaag aagagctgat ggacgctacg ggccagccta gtaagtggac
     4021 gaaccctcta ggttcgtgac ccttgcgagc agtcgtatgc tttctcgaat tccgagcggt
     4081 taagcgtgac aggcggtcag taggaggaac agtatggcag tcgaacataa tatgcagtca
     4141 agtacgattg tagatgtgct cgaaaagatt ttggataaag gagtcgttat agcgggggac
     4201 atcaccgtag gaattgcaga tgtcgagcta ttaacgataa agatccgctt gattgtggct
     4261 tcggttgata aggcaaaaga aatcggcatg gactggtggg aaaatgatcc gtatctcagt
     4321 tcaaaaggag ccaataacaa agcgctcgaa gaagaaaata aaatgctgca tgagcggtta
     4381 aaaacgcttg aagaaaaaat agaaacgaaa cgttaaaaac tgtacgctac ttaaaaaatg
     4441 gagggattta caatggcaac tgaaacaaaa ttagataaca cacaggcaga aaacaaggaa
     4501 aataaaaatg cggaaaacgg ttcaaaagaa aagaacggtt caaaagcaag caaaacaaca
     4561 agcagcgggc caatcaaacg agcggtagca ggaggcatca tcggtgcaac gattggatat
     4621 gtatcgactc ctgaaaatcg aaaaagtctc cttgaccgca ttgatacaga cgaattaaaa
     4681 agcaaagcat ctgatttagg aacaaaggta aaagaaaaat caaaaagcag cgtggccagc
     4741 ctgaaaacat ctgcgggaag cttgtttaaa aaagataaag ataaatcaaa agatgatgaa
     4801 gaaaacgtaa attcttctag tagcgaaaca gaagacgata acgttcaaga gtacgacgag
     4861 ttaaaagaag aaaatcaaac tcttcaagat cgcttatcac agcttgaaga aaaaatgaac
     4921 atgcttgttg agcttagcct caataaaaat caagacgaag aagcggaaga tacagattcc
     4981 gacgaagaag agaacgatga gaacgatgaa aacgatgaaa acgagcagga cgatgaaaac
     5041 gaagaagaaa catctaagcc acgtaaaaag gataaaaaag aagctgagga agaagaaagt
     5101 gaaagtgacg aagacagcga ggaagaagag gaagattctc gctcaaacaa aaaaaataaa
     5161 aaagtaaaaa cagaagaaga agacgaagat gaaagcgaag aagaaaaaaa ggaagcgaaa
     5221 ccaaaaaagt caacagctaa aaaatcaaaa aatacaaaag caaagaaaaa cacggacgaa
     5281 gaagatgatg aagcaacatc tctttctagt gaagacgata caacagccta agacgtaaag
     5341 gaggaaagaa agacatgagt acaggccctt ctttttcaac taaagacaat acgcttgaat
     5401 actttgtgaa agcttctaat aaacacggct tttcacttga tatttcatta aatgtaaacg
     5461 gcgctgtgat ttccggtacc atgatttcag caaaagaata ctttgattac ttaagcgaaa
     5521 cgtttgaaga aggcagtgaa gtggctcagg cgctaagcga acaattctct ttagcaagcg
     5581 aagcgagcga atcaaacgga gaagcagaag cccattttat tcatttgaaa aatacaaaga
     5641 tttactgtgg agacagtaaa tctactcctt ctaaaggcaa aatcttttgg agagggaaaa
     5701 tagcagaagt agacgggttt ttcttaggaa agatttctga tgcaaaatca acgagtaaaa
     5761 agagttcata aaaaacggcg gggtgattgc cccgccgttt tttagtgatg tgatgagatg
     5821 tgcagcttct tttttcgata catgagcgga gtcatcttca tttcttttcg aaactgatta
     5881 ataaaatagc ttgtactatt aaaccctact tgataggcga cttccgtcac atttgcttct
     5941 gcttgctgca gtccggattc gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg
     6001 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     6061 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg actagtagcc
     6121 cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca
     6181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6241 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6301 cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg
     6361 ggggcacgag tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt
     6421 cgccctgaag agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta
     6481 ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat
     6541 gacttggttg aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga
     6601 gaattatgta gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca
     6661 accatcggtg gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact
     6721 cgccttgacc gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc
     6781 acaatgcctg cggcaatggc aacaacatta cgcaaactat taactggcga actacttact
     6841 ctggcttcac ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta
     6901 ctgcgttcgg cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt
     6961 ggttcacggg gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt
     7021 atctacacga cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata
     7081 ggggcttcac tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt
     7141 tttttttgga cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7321 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7741 gctcgtcagg ggggcggagc ctatggaaa
//
