LOCUS       sequence-445441-        7753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLJ
FEATURES             Location/Qualifiers
     source          1..7753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6011..6029)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6025..6072
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6139..6230
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7095..7134
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7165..7753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7654..7673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagagtgag gaattaacat tatgtctctt aaacaatcca tggagaataa
     3781 agatattgct cttattgata ttttagatgt cattttagat aaaggagtcg ccattaaagg
     3841 agacttaatc atttccatag ctggcgtcga tttagtgtat ttggatttgc gggtgcttat
     3901 ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat cacaaaccaa tcacttctga
     3961 acaatttgat aaacaaaaag aagagctgat ggacgctacg ggccagccta gtaagtggac
     4021 gaaccctcta ggttcgtgac ccttgcgagc agtcgtatgc tttctcgaat tccgagcggt
     4081 taagcgtgac agtccaggag gaattaatag atgcaaccgg tcagccaagc aaatggacga
     4141 atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat gacagttatt
     4201 gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg tggaacgttg
     4261 acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga aaaaatggac
     4321 gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct tggcccgcta
     4381 ggcagcctgc tttaagcggt cagctgtacg ctacttaaaa aatggaggga tttacaatgg
     4441 caactgaaac aaaattagat aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa
     4501 acggttcaaa agaaaagaac ggttcaaaag caagcaaaac aacaagcagc gggccaatca
     4561 aacgagcggt agcaggaggc atcatcggtg caacgattgg atatgtatcg actcctgaaa
     4621 atcgaaaaag tctccttgac cgcattgata cagacgaatt aaaaagcaaa gcatctgatt
     4681 taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg
     4741 gaagcttgtt taaaaaagat aaagataaat caaaagatga tgaagaaaac gtaaattctt
     4801 ctagtagcga aacagaagac gataacgttc aagagtacga cgagttaaaa gaagaaaatc
     4861 aaactcttca agatcgctta tcacagcttg aagaaaaaat gaacatgctt gttgagctta
     4921 gcctcaataa aaatcaagac gaagaagcgg aagatacaga ttccgacgaa gaagagaacg
     4981 atgagaacga tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa gaaacatcta
     5041 agccacgtaa aaaggataaa aaagaagctg aggaagaaga aagtgaaagt gacgaagaca
     5101 gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag
     5161 aagaagacga agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag
     5221 ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa
     5281 catctctttc tagtgaagac gatacaacag cctaagacgt aaaggaggaa agaaagacat
     5341 gagtacaggc ccttcttttt caactaaaga caatacgctt gaatactttg tgaaagcttc
     5401 taataaacac ggcttttcac ttgatatttc attaaatgta aacggcgctg tgatttccgg
     5461 taccatgatt tcagcaaaag aatactttga ttacttaagc gaaacgtttg aagaaggcag
     5521 tgaagtggct caggcgctaa gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa
     5581 cggagaagca gaagcccatt ttattcattt gaaaaataca aagatttact gtggagacag
     5641 taaatctact ccttctaaag gcaaaatctt ttggagaggg aaaatagcag aagtagacgg
     5701 gtttttctta ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt cataaaaaac
     5761 ggcggggtga ttgccccgcc gttttttagt gatgtgatga gatgtgcagc ttcttttttc
     5821 gatacatgag cggagtcatc ttcatttctt ttcgaaactg attaataaaa tagcttgtac
     5881 tattaaaccc tacttgatag gcgacttccg tcacatttgc ttctgcttgc tgcagtccgg
     5941 attcgcgaac gcgtctcgag agatccggct gctaacaaag cccgaaagga agctgagttg
     6001 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     6061 aggggttttt tgctgaaagg aggaactata tccgactagt agcccgccta atgagcgggc
     6121 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6241 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6301 acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca cgagtgggat
     6361 acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct gaagagcgtt
     6421 ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc cgtgtagatg
     6481 cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg gttgaatact
     6541 ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta tgtagtgccg
     6601 ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc ggtggaccga
     6661 aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt gaccgttggg
     6721 aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg cctgcggcaa
     6781 tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct tcacggcaac
     6841 aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt tcggcacttc
     6901 ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca cggggtatca
     6961 ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac acgacgggta
     7021 gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct tcactgatta
     7081 agcattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacggacc
     7141 ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7741 gagcctatgg aaa
//
