LOCUS       sequence-445454-       11024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rho reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada 
            R, Umar M, Mavalwala K, Hayer A
  TITLE     Excitable Rho dynamics control cell shape and motility by 
            sequentially activating ERM proteins and actomyosin contractility.
  JOURNAL   Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. 
            Epub 2024 Sep 6.
  PUBMED    39241071
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-09-6"; volume: "10(36):eadn6858. doi"; pages: " 
            10.1126/sciadv.adn6858. Epub 2024 Sep 6"
COMMENT     Sequence Label: pLV-RhoB Sensor-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..11024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3392..3409
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3695..4426
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGSGSGSVSKGEELFTGVVPILVELDGDVNGHKFSVSGE
                     GEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEG
                     YVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV
                     YITADKQKNGIKANFKIRHNIE"
     primer_bind     3829..3850
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3953..3974)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4214..4233)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5988..6537
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6132..6149)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6359..6378
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6992..7580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7045..7065)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7463..7474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7787..8420
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8548..8564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8548..8564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8561..8583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8572..8588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8596..8602,8603..8620,8621..8626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8779..8796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8950..9538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9030..9049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9709..10500,10501..10569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10332..10351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10570..10674)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10722..10856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10759..10778)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10813..10832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11024,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccatggc acaccatcac caccatcacg gatctggttc
     3421 tagcctgggc cccgtagagc tgctgctgcg gggctcctcg cgccgcctcg acctgctgca
     3481 ccagcagctg caggagctgc acgcccacgg ttctggttcc ctgctagagc agctgggcct
     3541 ggccggggca gacctggcgg cccccggggt acagcagcag ctggagctgg agcgggagcg
     3601 gctgcggcgg gaaatccgca aggagctgaa gctgaaggag ggtgctgaga acctgcggcg
     3661 ggccaccact gacctgggcc gcggtaccgg ttctgacggc ggcgtgcagc tcgccgacca
     3721 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3781 gagctaccag tccgcactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3841 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggttctgg
     3901 aagtggttct gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3961 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4021 cacctacggc aagctgaccc tgaagttgat ctgcaccacc ggcaagctgc ccgtgccctg
     4081 gcccaccctc gtgaccaccc tcggctacgg cctgcagtgc ttcgcccgct accccgacca
     4141 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4201 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4261 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4321 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     4381 gaagaacggc atcaaggcaa acttcaagat ccgccacaac atcgagggat cagaagcaca
     4441 aaagcagaag gaacagagac aggcagcaga ggagctggca aacggatcgg aggctcagaa
     4501 acaaaaagag caaaggcaag ctgctgaaga actagctaat ggttctgagg cgcaaaagca
     4561 gaaagaacaa cgtcaggccg ccgaagagtt agcgaacgga tccgggagtg tttccaaagg
     4621 ggaagaattg tttaccggcg tggtccccat tctcgtggaa cttgatggcg atgtgaatgg
     4681 acacaaattc tccgtaagcg gagagggtga gggtgatgct acttacggga agttgactct
     4741 taagtttatt tgcacgactg ggaaactccc cgtcccttgg cctactctgg tgactacact
     4801 gagttgggga gttcaatgct ttgcccgata tcctgaccat atgaaacagc atgacttttt
     4861 taaatcagct atgcctgagg ggtatgttca ggaacgcact atctttttca aagacgatgg
     4921 gaattataaa accagggctg aagtgaaatt tgagggtgac acactggtca atcgaatcga
     4981 actgaaagga attgacttta aagaagatgg gaacattctt ggacacaaac tggaatacaa
     5041 tgcgatccac gggaacgtgt acatcactgc cgataaacag aaaaatggga tcaaagccaa
     5101 tttcggactt aattgtaaca ttgaagatgg cagtgtgcaa cttgccgatc actatcagca
     5161 aaataccccg atcggagacg gacctgtgct cctgccagat aaccattatc tgagtacgca
     5221 gtctgcactc agcaaggacc ctaatgagaa gagagaccat atggtgctcc tggaatttgt
     5281 caccgctgcc ggcgctagcg cggccatccg caagaagctg gtggttgtgg gcgacggcgc
     5341 gtgtggcaag acgtgcctgc tgatcgtgtt cagtaaggac gagttccccg aggtgtacgt
     5401 gcccaccgtc ttcgagaact atgtggccga cattgaggtg gacggcaagc aggtggagct
     5461 ggcgctgtgg gacacggcgg gccaggagga ctacgaccgc ctgcggccgc tctcctaccc
     5521 ggacaccgac gtcattctca tgtgcttctc ggtggacagc ccggactcgc tggagaacat
     5581 ccccgagaag tgggtccccg aggtgaagca cttctgtccc aatgtgccca tcatcctggt
     5641 ggccaacaaa aaagacctgc gcagcgacga gcatgtccgc acagagctgg cccgcatgaa
     5701 gcaggaaccc gtgcgcacgg atgacggccg cgccatggcc gtgcgcatcc aagcctacga
     5761 ctacctcgag tgctctgcca agaccaagga aggcgtgcgc gaggtcttcg agacggccac
     5821 gcgcgccgcg ctgcagaagc gctacggctc ccagaacggc tgcatcaact gctgcaaggt
     5881 gctatgagcg gccgctctag agtcgacggg ccgcggtaac aattgttaac taacttaagc
     5941 tagcaacggt ttccctctag cgggatcaat tccgcccccc ccccctaacg ttactggccg
     6001 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     6061 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     6121 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     6181 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     6241 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     6301 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     6361 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     6421 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     6481 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca
     6541 tggtcatgaa aacatttaac atttctcaac aagatctaga attagtagaa gtagcgacag
     6601 agaagattac aatgctttat gaggataata aacatcatgt gggagcggca attcgtacga
     6661 aaacaggaga aatcatttcg gcagtacata ttgaagcgta tataggacga gtaactgttt
     6721 gtgcagaagc cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat tttgacacga
     6781 ttgtagctgt tagacaccct tattctgacg aagtagatag aagtattcga gtggtaagtc
     6841 cttgtggtat gtgtagggag ttgatttcag actatgcacc agattgtttt gtgttaatag
     6901 aaatgaatgg caagttagtc aaaactacga ttgaagaact cattccactc aaatataccc
     6961 gaaattaacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     7021 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7081 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7141 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7201 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7261 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7321 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7381 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7441 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7501 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7561 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     7621 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7681 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7741 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7801 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7861 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7921 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7981 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8041 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     8101 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8161 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     8221 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8281 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8341 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8401 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8461 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     8521 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8701 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10681 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    10741 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10801 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10861 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10921 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10981 aaagaaattg tatttgttaa atatgtacta caaacttagt agtt
//
