LOCUS       sequence-445456-        7396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpL and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBLS
FEATURES             Location/Qualifiers
     source          1..7396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2927..2944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5654..5672)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5668..5715
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5782..5873
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6738..6777
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6808..7396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7297..7316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa
     1141 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     1201 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     1261 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     1321 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     1381 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     1441 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     1501 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     1561 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     1621 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     1681 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     1741 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     1801 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     1861 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     1921 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     1981 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     2041 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     2101 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     2161 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     2221 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     2281 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     2341 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     2401 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     2461 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     2521 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     2581 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     2641 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     2701 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     2761 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     2821 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     2881 aaaaagaagt caagacagat gtgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     2941 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     3001 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     3061 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     3121 aagagaaata acgtgcttca caaattagta accgcaccca ttaaccttgt agtgaaaatc
     3181 ggcgaaaaag tacaggaaga agctgataaa cagctatatg accttccgac gattcagcaa
     3241 aagctcattc agcttcaaat gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa
     3301 gaaaaagaag atgaattgtt aatgaggtac gaaattgcga aacgcagaga aattgaacaa
     3361 tgggaagagc taacacaaaa aagaaatgag gaatcctagg cggttaagcg tgacagtcca
     3421 ggaggaatta atggatgcaa ccggtcagcc aagcaaatgg acgaatccac ttggatcctg
     3481 atcaagctga acaaggctta gcgcagcttg tgatgacagt tattgagcta ttgaggcaaa
     3541 tcgttgaacg tcatgccatg aggcgggtgg agggtggaac gttgacggac gaacaaattg
     3601 aaaacttagg aattgcacta atgaacttag aagaaaaaat ggacgagttg aaagaggtgt
     3661 tcggtctgga tgcagaagat ttaaatattg atcttggacc gctaggcagc ctgctttaag
     3721 cggtcagtag gaggaacagt atggcagtcg aacataatat gcagtcaagt acgattgtag
     3781 atgtgctcga aaagattttg gataaaggag tcgttatagc gggggacatc accgtaggaa
     3841 ttgcagatgt cgagctatta acgataaaga tccgcttgat tgtggcttcg gttgataagg
     3901 caaaagaaat cggcatggac tggtgggaaa atgatccgta tctcagttca aaaggagcca
     3961 ataacaaagc gctcgaagaa gaaaataaaa tgctgcatga gcggttaaaa acgcttgaag
     4021 aaaaaataga aacgaaacgt taaaaactgt acgctactta aaaaatggag ggatttacaa
     4081 tggcaactga aacaaaatta gataacacac aggcagaaaa caaggaaaat aaaaatgcgg
     4141 aaaacggttc aaaagaaaag aacggttcaa aagcaagcaa aacaacaagc agcgggccaa
     4201 tcaaacgagc ggtagcagga ggcatcatcg gtgcaacgat tggatatgta tcgactcctg
     4261 aaaatcgaaa aagtctcctt gaccgcattg atacagacga attaaaaagc aaagcatctg
     4321 atttaggaac aaaggtaaaa gaaaaatcaa aaagcagcgt ggccagcctg aaaacatctg
     4381 cgggaagctt gtttaaaaaa gataaagata aatcaaaaga tgatgaagaa aacgtaaatt
     4441 cttctagtag cgaaacagaa gacgataacg ttcaagagta cgacgagtta aaagaagaaa
     4501 atcaaactct tcaagatcgc ttatcacagc ttgaagaaaa aatgaacatg cttgttgagc
     4561 ttagcctcaa taaaaatcaa gacgaagaag cggaagatac agattccgac gaagaagaga
     4621 acgatgagaa cgatgaaaac gatgaaaacg agcaggacga tgaaaacgaa gaagaaacat
     4681 ctaagccacg taaaaaggat aaaaaagaag ctgaggaaga agaaagtgaa agtgacgaag
     4741 acagcgagga agaagaggaa gattctcgct caaacaaaaa aaataaaaaa gtaaaaacag
     4801 aagaagaaga cgaagatgaa agcgaagaag aaaaaaagga agcgaaacca aaaaagtcaa
     4861 cagctaaaaa atcaaaaaat acaaaagcaa agaaaaacac ggacgaagaa gatgatgaag
     4921 caacatctct ttctagtgaa gacgatacaa cagcctaaga cgtaaaggag gaaagaaaga
     4981 catgagtaca ggcccttctt tttcaactaa agacaatacg cttgaatact ttgtgaaagc
     5041 ttctaataaa cacggctttt cacttgatat ttcattaaat gtaaacggcg ctgtgatttc
     5101 cggtaccatg atttcagcaa aagaatactt tgattactta agcgaaacgt ttgaagaagg
     5161 cagtgaagtg gctcaggcgc taagcgaaca attctcttta gcaagcgaag cgagcgaatc
     5221 aaacggagaa gcagaagccc attttattca tttgaaaaat acaaagattt actgtggaga
     5281 cagtaaatct actccttcta aaggcaaaat cttttggaga gggaaaatag cagaagtaga
     5341 cgggtttttc ttaggaaaga tttctgatgc aaaatcaacg agtaaaaaga gttcataaaa
     5401 aacggcgggg tgattgcccc gccgtttttt agtgatgtga tgagatgtgc agcttctttt
     5461 ttcgatacat gagcggagtc atcttcattt cttttcgaaa ctgattaata aaatagcttg
     5521 tactattaaa ccctacttga taggcgactt ccgtcacatt tgcttctgct tgctgcagtc
     5581 cggattcgcg aacgcgtctc gagagatccg gctgctaaca aagcccgaaa ggaagctgag
     5641 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     5701 ttgaggggtt ttttgctgaa aggaggaact atatccgact agtagcccgc ctaatgagcg
     5761 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5821 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5881 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5941 ctcacccaga aacgctcgtg aaagtaaaag acgcagagga ccaattgggg gcacgagtgg
     6001 gatacataga actggacttg aatagcggta aaatccttga gagttttcgc cctgaagagc
     6061 gttttccaat gatgagcact ttcaaagttc tgctatgtgg agcagtatta tcccgtgtag
     6121 atgcggggca agagcaactc ggacgacgaa tacactattc gcagaatgac ttggttgaat
     6181 actccccagt gacagaaaag caccttacgg acggaatgac ggtaagagaa ttatgtagtg
     6241 ccgccataac gatgagtgat aacactgcgg cgaacttact tctgacaacc atcggtggac
     6301 cgaaggaatt aaccgctttt ttgcacaata tgggagacca tgtaactcgc cttgaccgtt
     6361 gggaaccaga actgaatgaa gccataccaa acgacgagcg agacaccaca atgcctgcgg
     6421 caatggcaac aacattacgc aaactattaa ctggcgaact acttactctg gcttcacggc
     6481 aacaattaat agactggctt gaagcggata aagttgcagg accactactg cgttcggcac
     6541 ttcctgctgg ctggtttatt gctgataaat ctggggcagg agagcgtggt tcacggggta
     6601 tcattgccgc acttggacca gatggtaagc cttcccgtat cgtagttatc tacacgacgg
     6661 gtagtcaggc aactatggac gaacgaaata gacagattgc tgaaataggg gcttcactga
     6721 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacgg
     6781 accggtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6841 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6901 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6961 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7021 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7081 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7141 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7201 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7261 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7321 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7381 gcggagccta tggaaa
//
