LOCUS       sequence-445457-        7410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpL and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBLK
FEATURES             Location/Qualifiers
     source          1..7410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2927..2944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5668..5686)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5682..5729
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5796..5887
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6752..6791
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6822..7410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7311..7330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgctgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa
     1141 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     1201 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     1261 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     1321 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     1381 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     1441 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     1501 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     1561 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     1621 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     1681 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     1741 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     1801 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     1861 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     1921 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     1981 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     2041 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     2101 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     2161 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     2221 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     2281 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     2341 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     2401 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     2461 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     2521 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     2581 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     2641 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     2701 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     2761 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     2821 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     2881 aaaaagaagt caagacagat gtgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     2941 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     3001 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     3061 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     3121 aagagaaata acgtgcttca caaattagta accgcaccca ttaaccttgt agtgaaaatc
     3181 ggcgaaaaag tacaggaaga agctgataaa cagctatatg accttccgac gattcagcaa
     3241 aagctcattc agcttcaaat gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa
     3301 gaaaaagaag atgaattgtt aatgaggtac gaaattgcga aacgcagaga aattgaacaa
     3361 tgggaagagc taacacaaaa aagaaatgag gaatcctaga gtgaggaatt aacattatgt
     3421 ctcttaaaca atccatggag aataaagata ttgctcttat tgatatttta gatgtcattt
     3481 tagataaagg agtcgccatt aaaggagact taatcatttc catagctggc gtcgatttag
     3541 tgtatttgga tttgcgggtg cttatttctt cggttgaaac gcttgtgcaa gcaaaagaag
     3601 gaaatcacaa accaatcact tctgaacaat ttgataaaca aaaggaagag ttaatggacg
     3661 cgaccggcca gccgagcaaa tggaccaatc cgcttggctc ctgacccttg cgagcagtcg
     3721 tatgctttct cgaattccga gtaggaggaa cagtatggca gtcgaacata atatgcagtc
     3781 aagtacgatt gtagatgtgc tcgaaaagat tttggataaa ggagtcgtta tagcggggga
     3841 catcaccgta ggaattgcag atgtcgagct attaacgata aagatccgct tgattgtggc
     3901 ttcggttgat aaggcaaaag aaatcggcat ggactggtgg gaaaatgatc cgtatctcag
     3961 ttcaaaagga gccaataaca aagcgctcga agaagaaaat aaaatgctgc atgagcggtt
     4021 aaaaacgctt gaagaaaaaa tagaaacgaa acgttaaaaa ctgtacgcta cttaaaaaat
     4081 ggagggattt acaatggcaa ctgaaacaaa attagataac acacaggcag aaaacaagga
     4141 aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt tcaaaagcaa gcaaaacaac
     4201 aagcagcggg ccaatcaaac gagcggtagc aggaggcatc atcggtgcaa cgattggata
     4261 tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc attgatacag acgaattaaa
     4321 aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa tcaaaaagca gcgtggccag
     4381 cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa gataaatcaa aagatgatga
     4441 agaaaacgta aattcttcta gtagcgaaac agaagacgat aacgttcaag agtacgacga
     4501 gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca cagcttgaag aaaaaatgaa
     4561 catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa gaagcggaag atacagattc
     4621 cgacgaagaa gagaacgatg agaacgatga aaacgatgaa aacgagcagg acgatgaaaa
     4681 cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa gaagctgagg aagaagaaag
     4741 tgaaagtgac gaagacagcg aggaagaaga ggaagattct cgctcaaaca aaaaaaataa
     4801 aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa gaagaaaaaa aggaagcgaa
     4861 accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa gcaaagaaaa acacggacga
     4921 agaagatgat gaagcaacat ctctttctag tgaagacgat acaacagcct aagacgtaaa
     4981 ggaggaaaga aagacatgag tacaggccct tctttttcaa ctaaagacaa tacgcttgaa
     5041 tactttgtga aagcttctaa taaacacggc ttttcacttg atatttcatt aaatgtaaac
     5101 ggcgctgtga tttccggtac catgatttca gcaaaagaat actttgatta cttaagcgaa
     5161 acgtttgaag aaggcagtga agtggctcag gcgctaagcg aacaattctc tttagcaagc
     5221 gaagcgagcg aatcaaacgg agaagcagaa gcccatttta ttcatttgaa aaatacaaag
     5281 atttactgtg gagacagtaa atctactcct tctaaaggca aaatcttttg gagagggaaa
     5341 atagcagaag tagacgggtt tttcttagga aagatttctg atgcaaaatc aacgagtaaa
     5401 aagagttcat aaaaaacggc ggggtgattg ccccgccgtt ttttagtgat gtgatgagat
     5461 gtgcagcttc ttttttcgat acatgagcgg agtcatcttc atttcttttc gaaactgatt
     5521 aataaaatag cttgtactat taaaccctac ttgataggcg acttccgtca catttgcttc
     5581 tgcttgctgc agtccggatt cgcgaacgcg tctcgagaga tccggctgct aacaaagccc
     5641 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     5701 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc gactagtagc
     5761 ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc
     5821 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5881 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5941 ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag aggaccaatt
     6001 gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc ttgagagttt
     6061 tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat gtggagcagt
     6121 attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact attcgcagaa
     6181 tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa tgacggtaag
     6241 agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact tacttctgac
     6301 aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag accatgtaac
     6361 tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg agcgagacac
     6421 cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg aactacttac
     6481 tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg caggaccact
     6541 actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg caggagagcg
     6601 tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc gtatcgtagt
     6661 tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga ttgctgaaat
     6721 aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct tttggagcct
     6781 ttttttttgg acggaccggt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6841 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6901 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6961 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7021 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7081 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7141 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7201 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7261 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7321 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7381 tgctcgtcag gggggcggag cctatggaaa
//
