LOCUS       sequence-445463-        7054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNF
FEATURES             Location/Qualifiers
     source          1..7054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5312..5330)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5326..5373
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5440..5531
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6396..6435
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6466..7054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6955..6974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 ggtagaagag aaataacgtg cttcacaaat tagtaaccgc acccattaac cttgtagtga
     1801 aaatcggcga aaaagtacag gaagaagctg ataaacagct atatgacctt ccgacgattc
     1861 agcaaaagct cattcagctt caaatgatgt ttgagcttgg tgaaattcca gaagaagcgt
     1921 ttcaagaaaa agaagatgaa ttgttaatga ggtacgaaat tgcgaaacgc agagaaattg
     1981 aacaatggga agagctaaca caaaaaagaa atgaggaatc ctagatggga gaattactgt
     2041 atttatacgg tttaattcca acaaaagaag cagcagccat agagccgttt ccatcttata
     2101 aggggtttga cggagaacat tcactgtacc caattgcgtt tgatcaggtg acggctgtag
     2161 tttctaagct ggatgctgac acctattcag aaaaagtgat tcaagaaaaa atggagcagg
     2221 atatgagctg gctgcaggaa aaagcatttc atcatcacga aacggtagcc gctttgtacg
     2281 aagaatttac gatcattcca ttaaaatttt gcaccattta taaaggtgaa gaaagtctgc
     2341 aggcagctat tgagattaac aaagaaaaga tagagaattc actgacgctg cttcaaggaa
     2401 atgaagagtg gaatgtgaaa atttactgtg atgatacaga gcttaaaaaa ggaatcagcg
     2461 aaacgaatga aagcgtgaaa gcgaaaaaac aagaaattag tcacttatca ccaggaagac
     2521 agttttttga aaagaaaaaa atagatcagc tgattgaaaa agaattagag cttcacaaaa
     2581 acaaagtgtg tgaagagata catgacaagc taaaagaatt atcgctttat gactctgtta
     2641 aaaagaattg gagcaaagac gtaactggcg cagctgaaca gatggcgtgg aacagcgtgt
     2701 ttcttctccc gtctctgcag attactaagt tcgtaaacga aatagaagag cttcagcaaa
     2761 ggcttgaaaa taaaggctgg aagtttgaag tgacgggacc atggccgccc tatcatttct
     2821 cgagctttgc gtaaagtgag gaattaacat tatgtctctt aaacaatcca tggagaataa
     2881 agatattgct cttattgata ttttagatgt cattttagat aaaggagtcg ccattaaagg
     2941 agacttaatc atttccatag ctggcgtcga tttagtgtat ttggatttgc gggtgcttat
     3001 ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat cacaaaccaa tcacttctga
     3061 acaatttgat aaacaaaagg aggaattaat ggatgcaacc ggtcagccaa gcaaatggac
     3121 gaatccactt ggatcctgat caagctgaac aaggcttagc gcagcttgtg atgacagtta
     3181 ttgagctatt gaggcaaatc gttgaacgtc atgccatgag gcgggtggag ggtggaacgt
     3241 tgacggacga acaaattgaa aacttaggaa ttgcactaat gaacttagaa gaaaaaatgg
     3301 acgagttgaa agaggtgttc ggtctggatg cagaagattt aaatattgat cttggaccgc
     3361 taggcagcct gctttaagcg gtcagtagga ggaacagtat ggcagtcgaa cataatatgc
     3421 agtcaagtac gattgtagat gtgctcgaaa agattttgga taaaggagtc gttatagcgg
     3481 gggacatcac cgtaggaatt gcagatgtcg agctattaac gataaagatc cgcttgattg
     3541 tggcttcggt tgataaggca aaagaaatcg gcatggactg gtgggaaaat gatccgtatc
     3601 tcagttcaaa aggagccaat aacaaagcgc tcgaagaaga aaataaaatg ctgcatgagc
     3661 ggttaaaaac gcttgaagaa aaaatagaaa cgaaacgtta aaaactgtac gctacttaaa
     3721 aaatggaggg atttacaatg gcaactgaaa caaaattaga taacacacag gcagaaaaca
     3781 aggaaaataa aaatgcggaa aacggttcaa aagaaaagaa cggttcaaaa gcaagcaaaa
     3841 caacaagcag cgggccaatc aaacgagcgg tagcaggagg catcatcggt gcaacgattg
     3901 gatatgtatc gactcctgaa aatcgaaaaa gtctccttga ccgcattgat acagacgaat
     3961 taaaaagcaa agcatctgat ttaggaacaa aggtaaaaga aaaatcaaaa agcagcgtgg
     4021 ccagcctgaa aacatctgcg ggaagcttgt ttaaaaaaga taaagataaa tcaaaagatg
     4081 atgaagaaaa cgtaaattct tctagtagcg aaacagaaga cgataacgtt caagagtacg
     4141 acgagttaaa agaagaaaat caaactcttc aagatcgctt atcacagctt gaagaaaaaa
     4201 tgaacatgct tgttgagctt agcctcaata aaaatcaaga cgaagaagcg gaagatacag
     4261 attccgacga agaagagaac gatgagaacg atgaaaacga tgaaaacgag caggacgatg
     4321 aaaacgaaga agaaacatct aagccacgta aaaaggataa aaaagaagct gaggaagaag
     4381 aaagtgaaag tgacgaagac agcgaggaag aagaggaaga ttctcgctca aacaaaaaaa
     4441 ataaaaaagt aaaaacagaa gaagaagacg aagatgaaag cgaagaagaa aaaaaggaag
     4501 cgaaaccaaa aaagtcaaca gctaaaaaat caaaaaatac aaaagcaaag aaaaacacgg
     4561 acgaagaaga tgatgaagca acatctcttt ctagtgaaga cgatacaaca gcctaagacg
     4621 taaaggagga aagaaagaca tgagtacagg cccttctttt tcaactaaag acaatacgct
     4681 tgaatacttt gtgaaagctt ctaataaaca cggcttttca cttgatattt cattaaatgt
     4741 aaacggcgct gtgatttccg gtaccatgat ttcagcaaaa gaatactttg attacttaag
     4801 cgaaacgttt gaagaaggca gtgaagtggc tcaggcgcta agcgaacaat tctctttagc
     4861 aagcgaagcg agcgaatcaa acggagaagc agaagcccat tttattcatt tgaaaaatac
     4921 aaagatttac tgtggagaca gtaaatctac tccttctaaa ggcaaaatct tttggagagg
     4981 gaaaatagca gaagtagacg ggtttttctt aggaaagatt tctgatgcaa aatcaacgag
     5041 taaaaagagt tcataaaaaa cggcggggtg attgccccgc cgttttttag tgatgtgatg
     5101 agatgtgcag cttctttttt cgatacatga gcggagtcat cttcatttct tttcgaaact
     5161 gattaataaa atagcttgta ctattaaacc ctacttgata ggcgacttcc gtcacatttg
     5221 cttctgcttg ctgcagtccg gattcgcgaa cgcgtctcga gagatccggc tgctaacaaa
     5281 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     5341 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccgactag
     5401 tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac
     5461 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5521 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5581 tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa agtaaaagac gcagaggacc
     5641 aattgggggc acgagtggga tacatagaac tggacttgaa tagcggtaaa atccttgaga
     5701 gttttcgccc tgaagagcgt tttccaatga tgagcacttt caaagttctg ctatgtggag
     5761 cagtattatc ccgtgtagat gcggggcaag agcaactcgg acgacgaata cactattcgc
     5821 agaatgactt ggttgaatac tccccagtga cagaaaagca ccttacggac ggaatgacgg
     5881 taagagaatt atgtagtgcc gccataacga tgagtgataa cactgcggcg aacttacttc
     5941 tgacaaccat cggtggaccg aaggaattaa ccgctttttt gcacaatatg ggagaccatg
     6001 taactcgcct tgaccgttgg gaaccagaac tgaatgaagc cataccaaac gacgagcgag
     6061 acaccacaat gcctgcggca atggcaacaa cattacgcaa actattaact ggcgaactac
     6121 ttactctggc ttcacggcaa caattaatag actggcttga agcggataaa gttgcaggac
     6181 cactactgcg ttcggcactt cctgctggct ggtttattgc tgataaatct ggggcaggag
     6241 agcgtggttc acggggtatc attgccgcac ttggaccaga tggtaagcct tcccgtatcg
     6301 tagttatcta cacgacgggt agtcaggcaa ctatggacga acgaaataga cagattgctg
     6361 aaataggggc ttcactgatt aagcattggt aaaccgatac aattaaaggc tccttttgga
     6421 gccttttttt ttggacggac cggtagaaaa gatcaaagga tcttcttgag atcctttttt
     6481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6601 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7021 gtgatgctcg tcaggggggc ggagcctatg gaaa
//
