LOCUS       sequence-445469-        7301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpL and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBLJ
FEATURES             Location/Qualifiers
     source          1..7301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2927..2944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5558..5576)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5572..5619
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5686..5777
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6713..7301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7202..7221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa
     1141 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     1201 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     1261 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     1321 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     1381 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     1441 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     1501 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     1561 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     1621 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     1681 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     1741 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     1801 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     1861 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     1921 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     1981 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     2041 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     2101 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     2161 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     2221 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     2281 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     2341 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     2401 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     2461 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     2521 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     2581 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     2641 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     2701 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     2761 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     2821 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     2881 aaaaagaagt caagacagat gtgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     2941 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     3001 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     3061 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     3121 aagagaaata acgtgcttca caaattagta accgcaccca ttaaccttgt agtgaaaatc
     3181 ggcgaaaaag tacaggaaga agctgataaa cagctatatg accttccgac gattcagcaa
     3241 aagctcattc agcttcaaat gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa
     3301 gaaaaagaag atgaattgtt aatgaggtac gaaattgcga aacgcagaga aattgaacaa
     3361 tgggaagagc taccacaaaa aagaaatgag gaatcctaga gtgaggaatt aacattatgt
     3421 ctcttaaaca atccatggag aataaagata ttgctcttat tgatatttta gatgtcattt
     3481 tagataaagg agtcgccatt aaaggagact taatcatttc catagctggc gtcgatttag
     3541 tgtatttgga tttgcgggtg cttatttctt cggttgaaac gcttgtgcaa gcaaaagaag
     3601 gaaatcacaa accaatcact tctgaacaat ttgataaaca aaaggaggaa ttaatggatg
     3661 caaccggtca gccaagcaaa tggacgaatc cacttggatc ctgatcaagc tgaacaaggc
     3721 ttagcgcagc ttgtgatgac agttattgag ctattgaggc aaatcgttga acgtcatgcc
     3781 atgaggcggg tggagggtgg aacgttgacg gacgaacaaa ttgaaaactt aggaattgca
     3841 ctaatgaact tagaagaaaa aatggacgag ttgaaagagg tgttcggtct ggatgcagaa
     3901 gatttaaata ttgatcttgg accgctaggc agcctgcttt aagcggtcag ctgtacgcta
     3961 cttaaaaaat ggagggattt acaatggcaa ctgaaacaaa attagataac acacaggcag
     4021 aaaacaagga aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt tcaaaagcaa
     4081 gcaaaacaac aagcagcggg ccaatcaaac gagcggtagc aggaggcatc atcggtgcaa
     4141 cgattggata tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc attgatacag
     4201 acgaattaaa aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa tcaaaaagca
     4261 gcgtggccag cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa gataaatcaa
     4321 aagatgatga agaaaacgta aattcttcta gtagcgaaac agaagacgat aacgttcaag
     4381 agtacgacga gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca cagcttgaag
     4441 aaaaaatgaa catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa gaagcggaag
     4501 atacagattc cgacgaagaa gagaacgatg agaacgatga aaacgatgaa aacgagcagg
     4561 acgatgaaaa cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa gaagctgagg
     4621 aagaagaaag tgaaagtgac gaagacagcg aggaagaaga ggaagattct cgctcaaaca
     4681 aaaaaaataa aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa gaagaaaaaa
     4741 aggaagcgaa accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa gcaaagaaaa
     4801 acacggacga agaagatgat gaagcaacat ctctttctag tgaagacgat acaacagcct
     4861 aagacgtaaa ggaggaaaga aagacatgag tacaggccct tctttttcaa ctaaagacaa
     4921 tacgcttgaa tactttgtga aagcttctaa taaacacggc ttttcacttg atatttcatt
     4981 aaatgtaaac ggcgctgtga tttccggtac catgatttca gcaaaagaat actttgatta
     5041 cttaagcgaa acgtttgaag aaggcagtga agtggctcag gcgctaagcg aacaattctc
     5101 tttagcaagc gaagcgagcg aatcaaacgg agaagcagaa gcccatttta ttcatttgaa
     5161 aaatacaaag atttactgtg gagacagtaa atctactcct tctaaaggca aaatcttttg
     5221 gagagggaaa atagcagaag tagacgggtt tttcttagga aagatttctg atgcaaaatc
     5281 aacgagtaaa aagagttcat aaaaaacggc ggggtgattg ccccgccgtt ttttagtgat
     5341 gtgatgagat gtgcagcttc ttttttcgat acatgagcgg agtcatcttc atttcttttc
     5401 gaaactgatt aataaaatag cttgtactat taaaccctac ttgataggcg acttccgtca
     5461 catttgcttc tgcttgctgc agtccggatt cgcgaacgcg tctcgagaga tccggctgct
     5521 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     5581 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     5641 gactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct
     5701 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5761 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5821 cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag
     5881 aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc
     5941 ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat
     6001 gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact
     6061 attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa
     6121 tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact
     6181 tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag
     6241 accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg
     6301 agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg
     6361 aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg
     6421 caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg
     6481 caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc
     6541 gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga
     6601 ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct
     6661 tttggagcct tttttttttg gacggaccgg tagaaaagat caaaggatct tcttgagatc
     6721 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6781 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6841 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6901 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6961 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7021 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7081 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7141 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7201 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7261 gatttttgtg atgctcgtca ggggggcgga gcctatggaa a
//
