LOCUS       sequence-445471-        7879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB, GvpS and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBSK
FEATURES             Location/Qualifiers
     source          1..7879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2926..2943
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6137..6155)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6151..6198
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6265..6356
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7221..7260
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7291..7879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7780..7799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata gatctaacta ttggaggcta ctaaaaatgg aaattaaaaa
     1141 aattatgcaa gccgtgaacg actttttcgg tgaacacgta gctcctcctc ataaaattac
     1201 ctcggtggaa gctactgaag atgaaggttg gagagttatt gttgaagtca ttgaagaacg
     1261 agaatatatg aaaaaatacg ccaaagatga aatgctcgga acgtacgagt gctttgtaaa
     1321 taaagaaaaa gaagtcattt cattcaaacg actcgacgtc agatatagaa gcgccattgg
     1381 cattgaagca taaacgataa gatggcagga ggaacgtaaa aatgaccgtc ttaacagaca
     1441 aaaggaaaaa aggcagtgga gcttttatac aagatgacga gacaaaagag gttctttcaa
     1501 gagcgctgag ctatttaaaa tccggctatt ccattcattt tacaggtcct gccggcggag
     1561 gcaaaacctc tttagcgcga gcgcttgcta aaaagagaaa gcgtcctgta atgctgatgc
     1621 acgggaatca cgagctcaac aacaaagatt taattggcga ttttacggga tacacgagca
     1681 aaaaagtaat cgaccagtac gttcgttctg tctataaaaa agatgaacag gtgagtgaaa
     1741 actggcagga tggccgattg cttgaagctg taaaaaatgg ctatacgctg atttacgacg
     1801 aatttactcg ttctaagcct gcgacgaata atatctttct atcgatatta gaagaaggcg
     1861 tgctgccgct gtatggagta aaaatgaccg atccttttgt gcgcgtgcat cccgatttcc
     1921 gcgtcatctt cacaagcaat ccagctgagt atgccggcgt atatgatacg caagatgcgc
     1981 ttctcgacag gttaattacc atgtttattg attataaaga catcgacaga gagacagcga
     2041 ttttaacgga gaaaacggac gtagaagaag atgaagcgcg cacaattgta acgctcgtag
     2101 caaacgtgcg aaaccgctct ggagacgaaa acagcagcgg acttagcctg cgggcttcgc
     2161 ttatgatcgc tacccttgcc acgcagcaag acattcctat cgatggaagt gacgaagatt
     2221 ttcaaacgtt atgtatcgat attttgcatc atccgcttac caaatgtttg gatgaagaaa
     2281 atgcaaaaag caaagccgaa aaaatcattt tagaagaatg taagaatata gacactgaag
     2341 aaaagtaaag gagcttgaaa acatgagtga aacaaacgaa acaggtattt atatttttag
     2401 cgccattcaa acggataaag acgaagaatt tggcgccgtg gaagtagaag gaacaaaagc
     2461 tgaaacattt ttgattcgct acaaagacgc ggctatggta gcagctgaag taccgatgaa
     2521 aatttatcat cctaatcgcc aaaatttatt aatgcatcaa aacgcagtag cagcgattat
     2581 ggacaagaac gatacggtta ttccaatcag ctttgggaat gtattcaaat caaaagaaga
     2641 cgtaaaagtt cttttggaaa acctttatcc gcagtttgaa aagctgtttc cagcgatcaa
     2701 aggaaaaatt gaagtcggtt taaaagtaat tgggaaaaaa gaatggcttg agaaaaaagt
     2761 aaacgaaaat cctgaacttg agaaagtatc agcatccgta aaaggaaaat cagaagcagc
     2821 cggttattat gagcgtattc aacttggagg aatggctcaa aagatgttta cttccctgca
     2881 aaaagaagtc aagacagatg tgttttctcc gcttgaagaa gcagcggaag cagcaaaagc
     2941 aaatgagcca acgggcgaaa cgatgctttt aaacgcgtct ttcttaatta accgagaaga
     3001 tgaagcgaag tttgatgaaa aagtaaatga agcgcatgaa aactggaaag acaaagccga
     3061 ttttcattac agcggtcctt ggcctgctta taattttgtg aacattcgcc taaaagtaga
     3121 agagaaataa cgtgcttcac aaattagtaa ccgcacccat taaccttgta gtgaaaatcg
     3181 gcgaaaaagt acaggaagaa gctgataaac agctatatga ccttccgacg attcagcaaa
     3241 agctcattca gcttcaaatg atgtttgagc ttggtgaaat tccagaagaa gcgtttcaag
     3301 aaaaagaaga tgaattgtta atgaggtacg aaattgcgaa acgcagagaa attgaacaat
     3361 gggaagagct aacacaaaaa agaaatgagg aatcctagat gggagaatta ctgtatttat
     3421 acggtttaat tccaacaaaa gaagcagcag ccatagagcc gtttccatct tataaggggt
     3481 ttgacggaga acattcactg tacccaattg cgtttgatca ggtgacggct gtagtttcta
     3541 agctggatgc tgacacctat tcagaaaaag tgattcaaga aaaaatggag caggatatga
     3601 gctggctgca ggaaaaagca tttcatcatc acgaaacggt agccgctttg tacgaagaat
     3661 ttacgatcat tccattaaaa ttttgcacca tttataaagg tgaagaaagt ctgcaggcag
     3721 ctattgagat taacaaagaa aagatagaga attcactgac gctgcttcaa ggaaatgaag
     3781 agtggaatgt gaaaatttac tgtgatgata cagagcttaa aaaaggaatc agcgaaacga
     3841 atgaaagcgt gaaagcgaaa aaacaagaaa ttagtcactt atcaccagga agacagtttt
     3901 ttgaaaagaa aaaaatagat cagctgattg aaaaagaatt agagcttcac aaaaacaaag
     3961 tgtgtgaaga gatacatgac aagctaaaag aattatcgct ttatgactct gttaaaaaga
     4021 attggagcaa agacgtaact ggcgcagctg aacagatggc gtggaacagc gtgtttcttc
     4081 tcccgtctct gcagattact aagttcgtaa acgaaataga agagcttcag caaaggcttg
     4141 aaaataaagg ctggaagttt gaagtgacgg gaccatggcc gccctatcat ttctcgagct
     4201 ttgcgtaaag taggaggaac agtatggcag tcgaacataa tatgcagtca agtacgattg
     4261 tagatgtgct cgaaaagatt ttggataaag gagtcgttat agcgggggac atcaccgtag
     4321 gaattgcaga tgtcgagcta ttaacgataa agatccgctt gattgtggct tcggttgata
     4381 aggcaaaaga aatcggcatg gactggtggg aaaatgatcc gtatctcagt tcaaaaggag
     4441 ccaataacaa agcgctcgaa gaagaaaata aaatgctgca tgagcggtta aaaacgcttg
     4501 aagaaaaaat agaaacgaaa cgttaaaaac tgtacgctac ttaaaaaatg gagggattta
     4561 caatggcaac tgaaacaaaa ttagataaca cacaggcaga aaacaaggaa aataaaaatg
     4621 cggaaaacgg ttcaaaagaa aagaacggtt caaaagcaag caaaacaaca agcagcgggc
     4681 caatcaaacg agcggtagca ggaggcatca tcggtgcaac gattggatat gtatcgactc
     4741 ctgaaaatcg aaaaagtctc cttgaccgca ttgatacaga cgaattaaaa agcaaagcat
     4801 ctgatttagg aacaaaggta aaagaaaaat caaaaagcag cgtggccagc ctgaaaacat
     4861 ctgcgggaag cttgtttaaa aaagataaag ataaatcaaa agatgatgaa gaaaacgtaa
     4921 attcttctag tagcgaaaca gaagacgata acgttcaaga gtacgacgag ttaaaagaag
     4981 aaaatcaaac tcttcaagat cgcttatcac agcttgaaga aaaaatgaac atgcttgttg
     5041 agcttagcct caataaaaat caagacgaag aagcggaaga tacagattcc gacgaagaag
     5101 agaacgatga gaacgatgaa aacgatgaaa acgagcagga cgatgaaaac gaagaagaaa
     5161 catctaagcc acgtaaaaag gataaaaaag aagctgagga agaagaaagt gaaagtgacg
     5221 aagacagcga ggaagaagag gaagattctc gctcaaacaa aaaaaataaa aaagtaaaaa
     5281 cagaagaaga agacgaagat gaaagcgaag aagaaaaaaa ggaagcgaaa ccaaaaaagt
     5341 caacagctaa aaaatcaaaa aatacaaaag caaagaaaaa cacggacgaa gaagatgatg
     5401 aagcaacatc tctttctagt gaagacgata caacagccta agacgtaaag gaggaaagaa
     5461 agacatgagt acaggccctt ctttttcaac taaagacaat acgcttgaat actttgtgaa
     5521 agcttctaat aaacacggct tttcacttga tatttcatta aatgtaaacg gcgctgtgat
     5581 ttccggtacc atgatttcag caaaagaata ctttgattac ttaagcgaaa cgtttgaaga
     5641 aggcagtgaa gtggctcagg cgctaagcga acaattctct ttagcaagcg aagcgagcga
     5701 atcaaacgga gaagcagaag cccattttat tcatttgaaa aatacaaaga tttactgtgg
     5761 agacagtaaa tctactcctt ctaaaggcaa aatcttttgg agagggaaaa tagcagaagt
     5821 agacgggttt ttcttaggaa agatttctga tgcaaaatca acgagtaaaa agagttcata
     5881 aaaaacggcg gggtgattgc cccgccgttt tttagtgatg tgatgagatg tgcagcttct
     5941 tttttcgata catgagcgga gtcatcttca tttcttttcg aaactgatta ataaaatagc
     6001 ttgtactatt aaaccctact tgataggcga cttccgtcac atttgcttct gcttgctgca
     6061 gtccggattc gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg aaaggaagct
     6121 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     6181 gtcttgaggg gttttttgct gaaaggagga actatatccg actagtagcc cgcctaatga
     6241 gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc
     6301 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6361 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6421 ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag
     6481 tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag
     6541 agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg
     6601 tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg
     6661 aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta
     6721 gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg
     6781 gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc
     6841 gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg
     6901 cggcaatggc aacaacatta cgcaaactat taactggcga actacttact ctggcttcac
     6961 ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg
     7021 cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg
     7081 gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga
     7141 cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac
     7201 tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
     7261 cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7321 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7381 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7441 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7501 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7561 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7621 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7681 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7741 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7801 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7861 ggggcggagc ctatggaaa
//
