LOCUS       sequence-445473-        4087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G,
            M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M 
            Simpson E, R Leavitt B
  TITLE     New MiniPromoter Ple389 (ADORA2A) drives selective expression in 
            medium spiny neurons in mice and non-human primates.
  JOURNAL   Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y.
  PUBMED    39548191
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-15"; volume: "14(1):28194. doi"; pages: " 
            10.1038/s41598-024-79004-y"
COMMENT     Sequence Label: pEMS2368
FEATURES             Location/Qualifiers
     source          1..4087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     235..257
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     249..266
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     250..266
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1738..1754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1738..1754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1751..1773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1762..1778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1786..1792,1793..1810,1811..1816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1831..1852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1969..1986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2140..2728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2220..2239)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2899..3690,3691..3759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3522..3541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3760..3864)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3932..3950
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3988..4010)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
       61 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
      121 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
      181 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      241 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacctcgcga atgcatctag
      301 atggccggcc gaaaaatacc tagtcttgaa ctttggagtt ccaaattcca catgatcgac
      361 ttcctcaagg cttttcactt gacgaacaaa ttgcatgcca agaaatcgtc aaaattctat
      421 caagcttaat tatgcaattt ctgtctataa atgacatacc tcctgtaccc tatgacaccc
      481 acctatgcac taataagatg tgacagctgc atcaccaaca aagcctcccc cgcctgccat
      541 tctcttcgga ggtgagcacc tcctgctcgg gaggaactga cagagacagg aggaggtggg
      601 gacacagttc ccctactctg acaggaggca aggtgggaat aattggtact gatagatcta
      661 agtttatggg aggtgggagg aagttcatag agttcctacc tgacaaccta gattcatctg
      721 aaggaggagt gccagttaag agactgttac aagaagtatc gagaacctga gttgatgaca
      781 ttgggaacaa agctgtccca acctccccct tctccggggt ttcttttttg acctgatccc
      841 tcagtgatct ttgacgctgg gctttgctgc ccggcaggtg tcactcatcc ttcacccctc
      901 ccctcccagc tcaccctctt cgccgcctgg tctcagggtc ttcttcactc ctttctacct
      961 ctccttgtct ctctcccatc ccattcctgg aagttctgga ctggaggtgg ttgctctggc
     1021 ttctcctccc tcctaggacc ctcagatagt gttgggggca gggggagtgc ggcggggagg
     1081 gcacaggatg ggctggcgag gagagtgagt gctgggctcg caccgggagc cgggagcctg
     1141 acgtcaccac gccctgcctg tcaatctgca gcgcgcgccg ctcgcagccg ccttttctgc
     1201 caccaactgt atctctcact cgcggagccg gcacagcgac aggcgccccg cgcttgcctc
     1261 ctccccggcc cggccgcctc ctgcaggcag ctgctctgtt tgcgcgggga gctcagcccg
     1321 gcgccccgcc tcggcgccca tgaccagcga cttccagaag tcctgacgcc ccaggtgagt
     1381 ggccgagttc ccagggaact ttgaggatgt ggggagagga ggggagaaag atatccaggg
     1441 gacaggtctt tccagaagag gagaaatgga gccaaccttg actcccaccc tctgctgccc
     1501 ccatacacac accctagtct ctcctcccag acccccttcg caggggtctc tgggctccag
     1561 gactctccag gcttcgcaat gccagaaact tcgctttcag caccgcggac agcgtctctg
     1621 ctgcccagcc ccatggggat gtcctggtcg cgccgccaca acccaggcgg gaggcgcgcc
     1681 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt aatcatggtc
     1741 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1801 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1861 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1921 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1981 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2041 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2101 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2161 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2221 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2281 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2341 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2401 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2461 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2521 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2581 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2641 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2701 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2761 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3001 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3781 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3841 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3901 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     3961 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4021 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4081 tggcggg
//
