LOCUS       sequence-445479-        4380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G,
            M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M 
            Simpson E, R Leavitt B
  TITLE     New MiniPromoter Ple389 (ADORA2A) drives selective expression in 
            medium spiny neurons in mice and non-human primates.
  JOURNAL   Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y.
  PUBMED    39548191
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-15"; volume: "14(1):28194. doi"; pages: " 
            10.1038/s41598-024-79004-y"
COMMENT     Sequence Label: pEMS2374
FEATURES             Location/Qualifiers
     source          1..4380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(886..902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(886..902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(899..921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    910..926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(934..940,941..958,959..964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    979..1000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1117..1134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1288..1876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1368..1387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2047..2838,2839..2907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2670..2689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2908..3012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3080..3098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3136..3158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3258..3277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3471..3493
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3485..3502
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3486..3502
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agaaagaaag aaaaagaaag gaaggaagaa agaaagaaag aaaggaaaga aagaaagaga
       61 aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaaagaaaa
      121 gaaaagaaag aaaaggagag aggaagaaaa ggggagaaga acgaggttcc tctgcagccc
      181 aaatgacagt gggcagggta gcagcggaac acccttcacc actctatctc tttctccctc
      241 tccccactcc ctgcctcaca cacctcagtt tggagtcaag ggtgagctgg aacccctgag
      301 gcctcctttt gcatttccat gtcaggtcaa ctccaaacag gctttttcct ttggatgagt
      361 ccagcaatag aactgactac atagattcca agacctgaag tcagaaaacg gatgcggacc
      421 tcttccaaca cctcctctcc ttgcctgact tgctggatgg gtggtgcctc ccgcaaccct
      481 tggcttctga gtcctcaaag gagaagactg gcgagcagac ggtgaggacc cagcctgcaa
      541 tcacagctta ttactctggg tgtgggtggg agcgcagtgg gcggtcgagg gttgcgttcc
      601 cgcctcaaaa caagggatgg cggaatcccc caacccctcc tacccgttca ggccggggat
      661 cgccgaggag gtacagctcc tttggtgggg ggcgggggcg gggcctgtct caggggcggg
      721 gaccggggca cctccctccc gcgctccccg cgctcgggcg ccgcagagct gtccagcttc
      781 agtgccgaac cggcagcctc acgcgcgcac cgcgccgcct ccgccccgtc cccgcgctcc
      841 ctcctgcccg cccgccccgg gcgcgccaag cttggcgtaa tcatggtcat agctgtttcc
      901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      961 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1021 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2041 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2101 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2161 tggccccagt gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc
     2221 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2281 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2341 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2401 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2461 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2521 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2581 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2641 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2701 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2761 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2821 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2881 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2941 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3001 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3061 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttg cgcgtttcgg
     3121 tgatgacggt gaaaacctct gacacatgca gctcccggag actgtcacag cttgtctgta
     3181 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3241 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3301 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     3361 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3421 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     3481 acgttgtaaa acgacggcca gtgaattcgg ccggccaagg cagcaggaca ggcagctgat
     3541 gggcaggcct aggtaaaggc tctgactctc aaggttgcaa ggttgcccaa gtttcctgca
     3601 gtctctttac ctggcatcta ggtggcaggg ggcaggggtg tgagaaggca gaaagggcac
     3661 tttagaagca aggaagttag agccaccaag catgggctgg gggctgggct gacctttcct
     3721 ttcctttagc tgtgacgtga gctctagaaa tagaggcagg aaggaaggag ggtgaagggc
     3781 aggggcggga ggcggggcag gggaaagttg cccacagttg tcacgaggct tcatgtcttt
     3841 ctgccagaca gcagattgac agctagagtg ggcaggggga gggctgggct ccaccctctc
     3901 cccctctcag cacttcaggg gcaaagaaat gggaggagta ggacccgaca cgacacggga
     3961 acacaaggtg gaagggggtg gcccaggctc tgactctctg tcattggagg aaatgctgag
     4021 atgaagttaa tccttgtggc ccagagaggc tggattccag ccatgcaggg ccgactccct
     4081 gctctgcaga gtcagtcctc tgcccctgct cttccctctc cctggagtgt tgtgccacct
     4141 tctccccttg ggaactggtg ttgctccttc aatgcccggt tcctgtaaag tctcgctttt
     4201 ttgtgcttca cagcacctgt ttaagcctca gttatttccc tgggggtccc ctctctggat
     4261 tgccagggtg cgggatcagg ggcctggttt tctttctgtg tgtgacttct gaatctgaca
     4321 cagaatctgg caaactgagg acagttggta actaattgct gcagaagaaa gaaaagaaag
//
