LOCUS       sequence-44548-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS297
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     294..310
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     295..311
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(389..412)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agtctttttc nccctcactc gaatctcttg ttttgcaccg ttaccaactt ctcgtacaat
       61 gatgagttca actgtattct ggaaagtttt tagcaaggac actgcttgtc catgttcaat
      121 atttataaaa ctgtagccat tagcctgaat aattttatca cctggctgca gtaattttga
      181 tgctggtcct tcaggttgta cccttgttac aaatatacca tcatcatcag gtctgaatgg
      241 gtttcctcta cccccgacac cacctgatat gctaaatcca agttctggca ttgtcgaggt
      301 cgacggtatc gataagcttg atatccaatt cctgcagccc gggggatcca ctagtgcggc
      361 cgcttcagaa tccggggttt ttctccttga cgttaaagta tagaggtata ttaacaattt
      421 tttgttgata cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt
      481 attagttaaa gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca
      541 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
      601 atggttgtta atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt
      661 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
      721 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag
      781 tcttccttc
//
