LOCUS       sequence-445480-        7167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of transposase (CMV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pICOZ-P2_AG-Tn
FEATURES             Location/Qualifiers
     source          1..7167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        758..776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3540..3558)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3541..3558)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3578..3595)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3584..3808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3811..3827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3941..3960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4151..4172
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4291..4311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4606..4727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4643..4662)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4697..4716
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4776..4792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4789..4811)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4800..4816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4824..4830,4831..4848,4849..4854))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4869..4890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5007..5024)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5116..5132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(5178..5766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5258..5277)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6233..6611)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     6558..6577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6796..6867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6975..6994)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7128..7145
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7129..7145
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcgcgatccr ckwgtamsgk ssgmsrkykw tatrrgcaga gctctctggc
      721 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      781 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc
      841 cgccaccatg cctaacaaca gctgcgccgt gagcttttgt aagaacagca cctaccgggc
      901 caagacccag aacctgaaca tctccttcca ccggtttcct aagcatgagg atctgagaaa
      961 ccagtggaca ctgttctgcc agagggagga ggtgtggaag cccaaggata acgatgtgat
     1021 ttgcagctgc cactttacaa tcgagaacta ccagatggct aagagcccaa tcctcaagaa
     1081 ccggcagccc ctgaggatcc tgacccccaa cgccattccc agcctggtct gcaggaaccc
     1141 ccacaccatt cctgagcagg agcagattga tagagtggat gagcaccaga ggattaacga
     1201 gctgtacgag aggaacgaca gcaagagcgc ttccgacttc gtggaccacg aggccagcgt
     1261 cctgatcagc gatcaggaga agatccagaa cctcgaggcc gtgatcgcca cactgacaga
     1321 ggagattgac ttttgccgga agctgaggaa ggataacagc tttctgctga agcagaagaa
     1381 gattctgctg cagcagaaca agaagctgaa gctcaggaac acagtcctga agaaccggat
     1441 tagggtgctc gagcagacaa ccatccagca tcacgagatc gtgcccctca tgaagaagaa
     1501 gctgagtaag acactgagcg ggaaccagat cgatctcatc ctcaagagga agaagagggt
     1561 gcggtggaca aagaaggagc tgggggccgc cctgactctg cggtacttcg gcaagcggcc
     1621 ctacatgtac atgactgatg atatgcactt ccctctgccc gccagagcca caatccagcg
     1681 gtacattaag agcattaaca tcaagcaggg catcctggag gacgtcctga acctgatgga
     1741 gtcctacgcc aagacactga tccccaggga tagggagtgc gtgatctcct ttgacgagat
     1801 gaaggtcacc cacatcctgg agtacgacgt cggggccgat gaggtcctgg gcccccacaa
     1861 ctacctgcag gtggtcatgg ccaggggcat ctttaacaag tggaagcagc ccatcttcat
     1921 cggcttcgac cagaagatga ccaaggatat tatcctgagc ctgattaaga agctgagcga
     1981 gaggatgatt aacgtggtgg ccatcgtgag cgataactgc cagactaaca tcagctgctg
     2041 gcgggatctg ggcgcccacg acattaccaa gccctacttt aaccacccta tcaccaacaa
     2101 gaacgtgtac gtctttcctg acgcccccca tctgatcaag ctcctgcgga actggttgat
     2161 tgacacaggg tttgattaca aggggagcat catcaccgcc gacccactca gaaagctggt
     2221 ggaggagaga aaggatgccg agatcacccc actcttcaag ctgaacgaga accacttgac
     2281 catgagccca caggagcggc agaacgtcag gcgggccgtc cagctgctgt cccacaccac
     2341 cgctacagcc ctgcggaggt accagagcga cgatgccagc cagactctgg ccgagtttat
     2401 tgagaccgtc gattgctggt ttagcatctc caactcctac agcccttggg ccaagattgc
     2461 ctacaagcgg tcctacgccg ggaaggagga gcaggagaag tccctggaca agatgtacga
     2521 gctgatttcc aacatgaccg ccctgaacag gactagcatg cagaccttcc agaagtccat
     2581 cctcatgcac attacatccc tgaagatgct gtacaaggac atgcagaaga agcaccaggt
     2641 ggactttatc agcacacaca agctgaacca ggacattctg gagaacttct tcagccagct
     2701 gcgccagaag gggggggtct acgaccaccc atcccccctg agctgcctgc accggatccg
     2761 gatgatcgtg atcgggaagt cccccacaat cctgattaac cagactaacc tggagacaaa
     2821 ccggaacacc cggctgggag agaacatctg cctgatggag cagcacgaga acgccaacga
     2881 ggactgcctg tccgagcaga accaggactt catgagcgtg accctgttta ccgaggccga
     2941 tgtgattccc gatctgcccg acaacgccct gaacgcccac gaaatggatg atgagagcga
     3001 gatcctcagc accatcagca gcacaatccc tgacttgccc gagcaggaca gggatggctt
     3061 cgagtacatt atgggctacc tgggcaagaa gtttcacaag aagcaccctc acatcgacca
     3121 ggggacatac tccttcaaga ttacctcaga tcacaactac tccaagccac ccagctttgt
     3181 gaagcacctg agcacaggcg ggttgttcgt gcccagccac agcttcctga agctcggcag
     3241 caagatggag aagattttcc agaagctgca ccccgatggc actctggata agaagccagg
     3301 aatcgtcaag acaggcgtga acacaattaa gaagaagatc cccagcctgc ccgtggatat
     3361 gattaagtgt ttcatgatgc tgagagtgaa gctgaggatt aagcacacca acctgaaggc
     3421 cgctaacgag aagctgctga gatgcaagcg gaagggcccc aaggagcaca aggccgccaa
     3481 gaagatgaag aagattgtga actaagcggc cgctcgagca tgcatctaga gggccctatt
     3541 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     3601 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3661 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3721 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3781 catgctgggg atgcggtggg ctctatggcg gtcatagctg tttcctgtgt gggatatcct
     3841 cgagacagtg ggcccgttta aacwmrygks kywttmmryr sgsyssgysy ssykgwyacg
     3901 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3961 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4021 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4081 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4141 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4201 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4261 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4321 ccccaggctc cccagcaggc agaagtatgy aaagcatgca tctcaattag tcagcaacca
     4381 ggtgtggagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4441 aagctccccg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4501 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4561 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     4621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     4741 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4801 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4861 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4921 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4981 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5041 ctgcggcgag cggtatcagc tcaatcaarg gcggwmawwm gswkrymyrc wgrrkmwgsg
     5101 gwkrgctcta tggcggtcat agctgtttcc tgtgtgggat atcctcgaga cagtgggccc
     5161 gtttaaacac atgtgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5221 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5281 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5341 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5401 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5461 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5521 ctggcagcac ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5581 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatccgcgct
     5641 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5701 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag gaaaaaagga
     5761 tctcaagaag atcctttgat cttttctacg tcagtcctgc tcctcggcca cgaagtgcac
     5821 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     5881 ggtcatggcc ggcccggagg cgtccsgkaw rtwygwgkam ackwcstcyg acmgytcggc
     5941 rtacwgcwys wsyrrgscrc syaycymmrc satctgtcta tttcgttcat ccatagttgc
     6001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6841 tctcatgagc ggatacatat ttgaatgtrw kkmsawawat aaacaaatag gggttccgcg
     6901 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     6961 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     7021 gcttgtgtgt tgkmsktykc wrtaktaktg mrssakywaw mwkkrwkatw swmymatgmg
     7081 cggmtwmmww amssskyngc gaggccawaw gggttaaccc atggccttgt aaaacgacgg
     7141 ccagtcgacg ggccagatat acgcgtt
//
