LOCUS       sequence-445484-        8632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaG
FEATURES             Location/Qualifiers
     source          1..8632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..126
                     /gene="bla"
                     /label=AmpR promoter
     terminator      991..1030
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1061..1649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1550..1569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1803..1820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1835..1974
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1900..1919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2060..2082
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2076..2267)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2504..2523
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2504..2522
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4911..4928
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(8539..8557)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8553..8600
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca
       61 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      121 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      181 cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg
      241 ggggcacgag tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt
      301 cgccctgaag agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta
      361 ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat
      421 gacttggttg aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga
      481 gaattatgta gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca
      541 accatcggtg gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact
      601 cgccttgacc gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc
      661 acaatgcctg cggcaatggc aacaacatta cgcaaactat taactggcga actacttact
      721 ctggcttcac ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta
      781 ctgcgttcgg cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt
      841 ggttcacggg gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt
      901 atctacacga cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata
      961 ggggcttcac tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt
     1021 tttttttgga cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1201 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1741 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1801 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     1861 atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc
     1921 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1981 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2041 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2101 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     2161 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     2221 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     2281 taagggggat ttctgttcat ggggtaatga taccgatgaa acgagagagg atgctcacga
     2341 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     2401 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     2461 atacagatgt aggtgttggc accatgcatc gatcccgcga aattaatacg actcactata
     2521 gggagaccac aacggtttcc ctctagagat agcttagcga tgtgaaagaa gctggagaaa
     2581 actttcaaga aaaagtttct tcttcagacg ataaagagaa gtcaaaaaat aagcggaaaa
     2641 taaaaggcgt aaatcagatt aaaaaagcaa cggatattaa aagctctact aagattaaaa
     2701 gttctaatga tattaaatca tctacagatt taaaaacaat gggatcataa gcgaaaggag
     2761 aaatgagata tgtctattca aaaaagtact aatagttcaa gtttagcaga agtcattgac
     2821 cgtattttag ataaaggaat tgttattgat gcttttgcaa gagtttctgt tgtaggaatt
     2881 gaaattttaa cgattgaagc gcgagtggtt attgccagtg ttgatacatg gttacgctat
     2941 gcagaagcag tagggcttct tcgtgacgac gtagaagaaa acggtcttcc tgaacgttca
     3001 aattcaagtg aagggcagcc gcgttttagt atttaatatg aagtggtatg gaaccttcct
     3061 tttttgaggg tggttccagc ttttagatct aactattgga ggctactaaa aatggaaatt
     3121 aaaaaaatta tgcaagccgt gaacgacttt ttcggtgaac acgtagctcc tcctcataaa
     3181 attacctcgg tggaagctac tgaagatgaa ggttggagag ttattgttga agtcattgaa
     3241 gaacgagaat atatgaaaaa atacgccaaa gatgaaatgc tcggaacgta cgagtgcttt
     3301 gtaaataaag aaaaagaagt catttcattc aaacgactcg acgtcagata tagaagcgcc
     3361 attggcattg aagcataaac gataagatgg caggaggaac gtaaaaatga ccgtcttaac
     3421 agacaaaagg aaaaaaggca gtggagcttt tatacaagat gacgagacaa aagaggttct
     3481 ttcaagagcg ctgagctatt taaaatccgg ctattccatt cattttacag gtcctgccgg
     3541 cggaggcaaa acctctttag cgcgagcgct tgctaaaaag agaaagcgtc ctgtaatgct
     3601 gatgcacggg aatcacgagc tcaacaacaa agatttaatt ggcgatttta cgggatacac
     3661 gagcaaaaaa gtaatcgacc agtacgttcg ttctgtctat aaaaaagatg aacaggtgag
     3721 tgaaaactgg caggatggcc gattgcttga agctgtaaaa aatggctata cgctgattta
     3781 cgacgaattt actcgttcta agcctgcgac gaataatatc tttctatcga tattagaaga
     3841 aggcgtgctg ccgctgtatg gagtaaaaat gaccgatcct tttgtgcgcg tgcatcccga
     3901 tttccgcgtc atcttcacaa gcaatccagc tgagtatgcc ggcgtatatg atacgcaaga
     3961 tgcgcttctc gacaggttaa ttaccatgtt tattgattat aaagacatcg acagagagac
     4021 agcgatttta acggagaaaa cggacgtaga agaagatgaa gcgcgcacaa ttgtaacgct
     4081 cgtagcaaac gtgcgaaacc gctctggaga cgaaaacagc agcggactta gcctgcgggc
     4141 ttcgcttatg atcgctaccc ttgccacgca gcaagacatt cctatcgatg gaagtgacga
     4201 agattttcaa acgttatgta tcgatatttt gcatcatccg cttaccaaat gtttggatga
     4261 agaaaatgca aaaagcaaag ccgaaaaaat cattttagaa gaatgtaaga atatagacac
     4321 tgaagaaaag taaaggagct tgaaaacatg agtgaaacaa acgaaacagg tatttatatt
     4381 tttagcgcca ttcaaacgga taaagacgaa gaatttggcg ccgtggaagt agaaggaaca
     4441 aaagctgaaa catttttgat tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg
     4501 atgaaaattt atcatcctaa tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg
     4561 attatggaca agaacgatac ggttattcca atcagctttg ggaatgtatt caaatcaaaa
     4621 gaagacgtaa aagttctttt ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg
     4681 atcaaaggaa aaattgaagt cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa
     4741 aaagtaaacg aaaatcctga acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa
     4801 gcagccggtt attatgagcg tattcaactt ggaggaatgg ctcaaaagat gtttacttcc
     4861 ctgcaaaaag aagtcaagac agatgtgttt tctccgcttg aagaagcagc ggaagcagca
     4921 aaagcaaatg agccaacggg cgaaacgatg cttttaaacg cgtctttctt aattaaccga
     4981 gaagatgaag cgaagtttga tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa
     5041 gccgattttc attacagcgg tccttggcct gcttataatt ttgtgaacat tcgcctaaaa
     5101 gtagaagaga aataacgaaa tgaggaatcc tagatgggag aattactgta tttatacggt
     5161 ttaattccaa caaaagaagc agcagccata gagccgtttc catcttataa ggggtttgac
     5221 ggagaacatt cactgtaccc aattgcgttt gatcaggtga cggctgtagt ttctaagctg
     5281 gatgctgaca cctattcaga aaaagtgatt caagaaaaaa tggagcagga tatgagctgg
     5341 ctgcaggaaa aagcatttca tcatcacgaa acggtagccg ctttgtacga agaatttacg
     5401 atcattccat taaaattttg caccatttat aaaggtgaag aaagtctgca ggcagctatt
     5461 gagattaaca aagaaaagat agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg
     5521 aatgtgaaaa tttactgtga tgatacagag cttaaaaaag gaatcagcga aacgaatgaa
     5581 agcgtgaaag cgaaaaaaca agaaattagt cacttatcac caggaagaca gttttttgaa
     5641 aagaaaaaaa tagatcagct gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt
     5701 gaagagatac atgacaagct aaaagaatta tcgctttatg actctgttaa aaagaattgg
     5761 agcaaagacg taactggcgc agctgaacag atggcgtgga acagcgtgtt tcttctcccg
     5821 tctctgcaga ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat
     5881 aaaggctgga agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg
     5941 taaagtgagg aattaacatt atgtctctta aacaatccat ggagaataaa gatattgctc
     6001 ttattgatat tttagatgtc attttagata aaggagtcgc cattaaagga gacttaatca
     6061 tttccatagc tggcgtcgat ttagtgtatt tggatttgcg ggtgcttatt tcttcggttg
     6121 aaacgcttgt gcaagcaaaa gaaggaaatc acaaaccaat cacttctgaa caatttgata
     6181 aacaaaaaga agagctgatg gacgctacgg gccagcctag taagtggacg aaccctctag
     6241 gttcgtgacc cttgcgagca gtcgtatgct ttctcgaatt ccgagcggtt aagcgtgaca
     6301 gtccaggagg aattaataga tgcaaccggt cagccaagca aatggacgaa tccacttgga
     6361 tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg acagttattg agctattgag
     6421 gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga cggacgaaca
     6481 aattgaaaac ttaggaattg cactaatgaa cttagaagaa aaaatggacg agttgaaaga
     6541 ggtgttcggt ctggatgcag aagatttaaa tattgatctt ggcccgctag gcagcctgct
     6601 ttaagcggtc agtaggagga acagtatggc agtcgaacat aatatgcagt caagtacgat
     6661 tgtagatgtg ctcgaaaaga ttttggataa aggagtcgtt atagcggggg acatcaccgt
     6721 aggaattgca gatgtcgagc tattaacgat aaagatccgc ttgattgtgg cttcggttga
     6781 taaggcaaaa gaaatcggca tggactggtg ggaaaatgat ccgtatctca gttcaaaagg
     6841 agccaataac aaagcgctcg aagaagaaaa taaaatgctg catgagcggt taaaaacgct
     6901 tgaagaaaaa atagaaacga aacgttaaaa actgtacgct acttaaaaaa tggagggatt
     6961 tacaatggca actgaaacaa aattagataa cacacaggca gaaaacaagg aaaataaaaa
     7021 tgcggaaaac ggttcaaaag aaaagaacgg ttcaaaagca agcaaaacaa caagcagcgg
     7081 gccaatcaaa cgagcggtag caggaggcat catcggtgca acgattggat atgtatcgac
     7141 tcctgaaaat cgaaaaagtc tccttgaccg cattgataca gacgaattaa aaagcaaagc
     7201 atctgattta ggaacaaagg taaaagaaaa atcaaaaagc agcgtggcca gcctgaaaac
     7261 atctgcggga agcttgttta aaaaagataa agataaatca aaagatgatg aagaaaacgt
     7321 aaattcttct agtagcgaaa cagaagacga taacgttcaa gagtacgacg agttaaaaga
     7381 agaaaatcaa actcttcaag atcgcttatc acagcttgaa gaaaaaatga acatgcttgt
     7441 tgagcttagc ctcaataaaa atcaagacga agaagcggaa gatacagatt ccgacgaaga
     7501 agagaacgat gagaacgatg aaaacgatga aaacgagcag gacgatgaaa acgaagaaga
     7561 aacatctaag ccacgtaaaa aggataaaaa agaagctgag gaagaagaaa gtgaaagtga
     7621 cgaagacagc gaggaagaag aggaagattc tcgctcaaac aaaaaaaata aaaaagtaaa
     7681 aacagaagaa gaagacgaag atgaaagcga agaagaaaaa aaggaagcga aaccaaaaaa
     7741 gtcaacagct aaaaaatcaa aaaatacaaa agcaaagaaa aacacggacg aagaagatga
     7801 tgaagcaaca tctctttcta gtgaagacga tacaacagcc taagacgtaa aggaggaaag
     7861 aaagacatga gtacaggccc ttctttttca actaaagaca atacgcttga atactttgtg
     7921 aaagcttcta ataaacacgg cttttcactt gatatttcat taaatgtaaa cggcgctgtg
     7981 atttccggta ccatgatttc agcaaaagaa tactttgatt acttaagcga aacgtttgaa
     8041 gaaggcagtg aagtggctca ggcgctaagc gaacaattct ctttagcaag cgaagcgagc
     8101 gaatcaaacg gagaagcaga agcccatttt attcatttga aaaatacaaa gatttactgt
     8161 ggagacagta aatctactcc ttctaaaggc aaaatctttt ggagagggaa aatagcagaa
     8221 gtagacgggt ttttcttagg aaagatttct gatgcaaaat caacgagtaa aaagagttca
     8281 taaaaaacgg cggggtgatt gccccgccgt tttttagtga tgtgatgaga tgtgcagctt
     8341 cttttttcga tacatgagcg gagtcatctt catttctttt cgaaactgat taataaaata
     8401 gcttgtacta ttaaacccta cttgataggc gacttccgtc acatttgctt ctgcttgctg
     8461 cagtccggat tcgcgaacgc gtctcgagag atccggctgc taacaaagcc cgaaaggaag
     8521 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     8581 gggtcttgag gggttttttg ctgaaaggag gaactatatc cgactagtag cc
//
