LOCUS       sequence-445540-        7449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL, GvpS and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLSJ
FEATURES             Location/Qualifiers
     source          1..7449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5707..5725)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5721..5768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5835..5926
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6791..6830
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6861..7449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7350..7369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agggctaaca caaaaaagaa
     3721 atgaggaatc ctagaccctt gcgagcagtc gtatgctttc tcgaattccg agcggttaag
     3781 cgtgacagtc caggaggaat taatagatgc aaccggtcag ccaagcaaat ggacgaatcc
     3841 acttggatcc tgatcaagct gaacaaggct tagcgcagct tgtgatgaca gttattgagc
     3901 tattgaggca aatcgttgaa cgtcatgcca tgaggcgggt ggagggtgga acgttgacgg
     3961 acgaacaaat tgaaaactta ggaattgcac taatgaactt agaagaaaaa atggacgagt
     4021 tgaaagaggt gttcggtctg gatgcagaag atttaaatat tgatcttggc ccgctaggca
     4081 gcctgcttta agcggtcagc tgtacgctac ttaaaaaatg gagggattta caatggcaac
     4141 tgaaacaaaa ttagataaca cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg
     4201 ttcaaaagaa aagaacggtt caaaagcaag caaaacaaca agcagcgggc caatcaaacg
     4261 agcggtagca ggaggcatca tcggtgcaac gattggatat gtatcgactc ctgaaaatcg
     4321 aaaaagtctc cttgaccgca ttgatacaga cgaattaaaa agcaaagcat ctgatttagg
     4381 aacaaaggta aaagaaaaat caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag
     4441 cttgtttaaa aaagataaag ataaatcaaa agatgatgaa gaaaacgtaa attcttctag
     4501 tagcgaaaca gaagacgata acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac
     4561 tcttcaagat cgcttatcac agcttgaaga aaaaatgaac atgcttgttg agcttagcct
     4621 caataaaaat caagacgaag aagcggaaga tacagattcc gacgaagaag agaacgatga
     4681 gaacgatgaa aacgatgaaa acgagcagga cgatgaaaac gaagaagaaa catctaagcc
     4741 acgtaaaaag gataaaaaag aagctgagga agaagaaagt gaaagtgacg aagacagcga
     4801 ggaagaagag gaagattctc gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga
     4861 agacgaagat gaaagcgaag aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa
     4921 aaaatcaaaa aatacaaaag caaagaaaaa cacggacgaa gaagatgatg aagcaacatc
     4981 tctttctagt gaagacgata caacagccta agacgtaaag gaggaaagaa agacatgagt
     5041 acaggccctt ctttttcaac taaagacaat acgcttgaat actttgtgaa agcttctaat
     5101 aaacacggct tttcacttga tatttcatta aatgtaaacg gcgctgtgat ttccggtacc
     5161 atgatttcag caaaagaata ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa
     5221 gtggctcagg cgctaagcga acaattctct ttagcaagcg aagcgagcga atcaaacgga
     5281 gaagcagaag cccattttat tcatttgaaa aatacaaaga tttactgtgg agacagtaaa
     5341 tctactcctt ctaaaggcaa aatcttttgg agagggaaaa tagcagaagt agacgggttt
     5401 ttcttaggaa agatttctga tgcaaaatca acgagtaaaa agagttcata aaaaacggcg
     5461 gggtgattgc cccgccgttt tttagtgatg tgatgagatg tgcagcttct tttttcgata
     5521 catgagcgga gtcatcttca tttcttttcg aaactgatta ataaaatagc ttgtactatt
     5581 aaaccctact tgataggcga cttccgtcac atttgcttct gcttgctgca gtccggattc
     5641 gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     5701 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     5761 gttttttgct gaaaggagga actatatccg actagtagcc cgcctaatga gcgggctttt
     5821 ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6001 agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag tgggatacat
     6061 agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag agcgttttcc
     6121 aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg tagatgcggg
     6181 gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg aatactcccc
     6241 agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta gtgccgccat
     6301 aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg gaccgaagga
     6361 attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc gttgggaacc
     6421 agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg cggcaatggc
     6481 aacaacatta cgcaaactat taactggcga actacttact ctggcttcac ggcaacaatt
     6541 aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg cacttcctgc
     6601 tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg gtatcattgc
     6661 cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga cgggtagtca
     6721 ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac tgattaagca
     6781 ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga cggaccggta
     6841 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6901 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6961 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7021 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7081 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7141 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7201 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7261 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7321 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7381 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7441 ctatggaaa
//
