LOCUS       sequence-445551-        1683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded fluorescent biosensor for nicotine detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Haloi N, Huang S, Nichols AL, Fine EJ, Friesenhahn NJ, Marotta CB, 
            Dougherty DA, Lindahl E, Howard RJ, Mayo SL, Lester HA
  TITLE     Interactive computational and experimental approaches improve the 
            sensitivity of periplasmic binding protein-based nicotine biosensors
            for measurements in biofluids.
  JOURNAL   Protein Eng Des Sel. 2024 Jan 29;37:gzae003. doi: 
            10.1093/protein/gzae003.
  PUBMED    38302088
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel."; date: "2024-01-29"; volume: "37:gzae003. doi"; pages: " 
            10.1093/protein/gzae003"
COMMENT     Sequence Label: iNicSnFR12
FEATURES             Location/Qualifiers
     source          1..1683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             25..51
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     516..537
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1648..1677
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgcatcatc atcatcatca tggttatccc tatgatgttc cagattatgc tggggcccag
       61 ccggccagat ctgcgaacga caccgtagtt gtgggctcga tcgagtttac agaagggatt
      121 atcgtcgcaa acatggtggc agagatgatt gaggcgcata cagaccttaa ggtggttcgc
      181 aaactgaacc ttggcgggga gaacgttaac tttgaagcca ttaaacgcgg aggtgcgaat
      241 aatggtattg acatttacgt ggagtacact gggcacggtc ttgtggatat tctggggttc
      301 ccggagccga acgtctatat caccgccgac aagcagaaga acggcatcaa ggcgaacttc
      361 aagatccgcc acaacatgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      421 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      481 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      541 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
      601 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcggc
      661 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
      721 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      781 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      841 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag
      901 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      961 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1021 gagtacaact ttccgccgcc cagctctact gatccagaag gtgcatacga aaccgtgaag
     1081 aaggagtaca aacgtaaatg gaatattgta tggctcaaac cactgggatt caacaatacg
     1141 tatacgctta ccgttaaaga cgaactggcg aaacagtata accttaaaac cttcagtgac
     1201 ttagcgaaaa tctcggataa gctgattctg ggtgcaacga tgttcttttt agaagggccc
     1261 gatggttacc caggcctgca aaaactgtac aatttcaaat tcaagcacac caaaagcatg
     1321 gacatgggta ttcgctatac cgccattgat aataacgaag ttgatgtaat tgatgcctgg
     1381 gccactgatg gcttgctggt gagccacaaa ttaaaaattc tggaggatga taaagcgttc
     1441 ttcccgccgt attatgctgc ccccatcatc agtcaggatg tcttagataa gcatcctaag
     1501 ctgaaggacg tgctgaacaa actcgcgaat caaatttcac tggaagaaat gcagaaactg
     1561 aattacaagg tggacggtga gggtcaggac ccagcgaaag tagctaagga gtttttgaaa
     1621 gagaaaggtt taattctgca ggtcgacgaa caaaaactca tctcagaaga ggatctgaat
     1681 taa
//
