LOCUS       sequence-445553-       10427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-LYS
FEATURES             Location/Qualifiers
     source          1..10427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        294..533
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             534..4715
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKEDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQ
                     FGLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDF
                     VSSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAG
                     ERGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVAR
                     VVVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVI
                     DRNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEV
                     GIYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     rep_origin      complement(5147..5602)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5284..5305)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5496..5515
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(5681..5846)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5767..5791
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5994..6017)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6064..6728
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    6280..6397
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     6692..6715
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     6739..6758
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6739..6757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6775..6804
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      6834..7023
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7095..7112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7266..7854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7346..7365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8025..8816,8817..8885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8648..8667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8886..8990)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9017..10359
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9980..10027)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10400..10418
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccag cttttcaatt caattcagcc agcggaattc cacttacagc ttttcaattc
      241 aattctgcca gcggaattcc acttacagct tttcaattca attcagccag cggaattcca
      301 cttgcaatta cataaaaaat tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct
      361 gcctaatgaa catgaacatc gcccaaatgt atttgaagac ccgctgggag aagttcaaga
      421 tatataagta acaagcagcc aatagtataa aaaaaaatct gagtttatta cctttcctgg
      481 aatttcagtg aaaaactgct aattatagag agatatcaca gagttactca ctaatgacta
      541 acgaaaaggt ctggatagag aagttggata atccaactct ttcagtgtta ccacatgact
      601 ttttacgccc acaacaagaa ccttatacga aacaagctac atattcgtta cagctacctc
      661 agctcgatgt gcctcatgat agtttttcta acaaatacgc tgtcgctttg agtgtatggg
      721 ctgcattgat atatagagta accggtgacg atgatattgt tctttatatt gcgaataaca
      781 aaatcttaag attcaatatt caaccaacgt ggtcatttaa tgagctgtat tctacaatta
      841 acaatgagtt gaacaagctc aattctattg aggccaattt ttcctttgac gagctagctg
      901 aaaaaattca aagttgccaa gacctggaaa ggacccctca gttgttccgt ttggcctttt
      961 tggaaaacca agatttcaaa ttagacgagt tcaagcatca tttagtggac tttgctttga
     1021 atttggatac cagtaataat gcgcatgttt tgaacttaat ttataacagc ttactgtatt
     1081 cgaatgaaag agtaaccatt gttgcggacc aatttactca atatttgact gctgcgctaa
     1141 gcgatccatc caattgcata actaaaatct ctctgatcac cgcatcatcc aaggatagtt
     1201 tacctgatcc aactaagaac ttgggctggt gcgatttcgt ggggtgtatt cacgacattt
     1261 tccaggacaa tgctgaagcc ttcccagaga gaacctgtgt tgtggagact ccaacactaa
     1321 attccgacaa gtcccgttct ttcacttatc gcgacatcaa ccgcacttct aacatagttg
     1381 cccattattt gattaaaaca ggtatcaaaa gaggtgatgt agtgatgatc tattcttcta
     1441 ggggtgtgga tttgatggta tgtgtgatgg gtgtcttgaa agccggcgca accttttcag
     1501 ttatcgaccc tgcatatccc ccagccagac aaaccattta cttaggtgtt gctaaaccac
     1561 gtgggttgat tgttattaga gctgctggac aattggatca actagtagaa gattacatca
     1621 atgatgaatt ggagattgtt tcaagaatca attccatcgc tattcaagaa aatggtacca
     1681 ttgaaggtgg caaattggac aatggcgagg atgttttggc tccatatgat cactacaaag
     1741 acaccagaac aggtgttgta gttggaccag attccaaccc aaccctatct ttcacatctg
     1801 gttccgaagg tattcctaag ggtgttcttg gtagacattt ttccttggct tattatttca
     1861 attggatgtc caaaaggttc aacttaacag aaaatgataa attcacaatg ctgagcggta
     1921 ttgcacatga tccaattcaa agagatatgt ttacaccatt atttttaggt gcccaattgt
     1981 atgtccctac tcaagatgat attggtacac cgggccgttt agcggaatgg atgagtaagt
     2041 atggttgcac agttacccat ttaacacctg ccatgggtca attacttact gcccaagcta
     2101 ctacaccatt ccctaagtta catcatgcgt tctttgtggg tgacatttta acaaaacgtg
     2161 attgtctgag gttacaaacc ttggcagaaa attgccgtat tgttaatatg tacggtacca
     2221 ctgaaacaca gcgtgcagtt tcttatttcg aagttaaatc aaaaaatgac gatccaaact
     2281 ttttgaaaaa attgaaagat gtcatgcctg ctggtaaagg tatgttgaac gttcagctac
     2341 tagttgttaa caggaacgat cgtactcaaa tatgtggtat tggcgaaata ggtgagattt
     2401 atgttcgtgc aggtggtttg gccgaaggtt atagaggatt accagaattg aataaagaaa
     2461 aatttgtgaa caactggttt gttgaaaaag atcactggaa ttatttggat aaggataatg
     2521 gtgaaccttg gagacaattc tggttaggtc caagagatag attgtacaga acgggtgatt
     2581 taggtcgtta tctaccaaac ggtgactgtg aatgttgcgg tagggctgat gatcaagtta
     2641 aaattcgtgg gttcagaatc gaattaggag aaatagatac gcacatttcc caacatccat
     2701 tggtaagaga aaacattact ttagttcgca aaaatgccga caatgagcca acattgatca
     2761 catttatggt cccaagattt gacaagccag atgacttgtc taagttccaa agtgatgttc
     2821 caaaggaggt tgaaactgac cctatagtta agggcttaat cggttaccat cttttatcca
     2881 aggacatcag gactttctta aagaaaagat tggctagcta tgctatgcct tccttgattg
     2941 tggttatgga taaactacca ttgaatccaa atggtaaagt tgataagcct aaacttcaat
     3001 tcccaactcc caagcaatta aatttggtag ctgaaaatac agtttctgaa actgacgact
     3061 ctcagtttac caatgttgag cgcgaggtta gagacttatg gttaagtata ttacctacca
     3121 agccagcatc tgtatcacca gatgattcgt ttttcgattt aggtggtcat tctatcttgg
     3181 ctaccaaaat gatttttacc ttaaagaaaa agctgcaagt tgatttacca ttgggcacaa
     3241 ttttcaagta tccaacgata aaggcctttg ccgcggaaat tgacagaatt aaatcatcgg
     3301 gtggatcatc tcaaggtgag gtcgtcgaaa atgtcactgc aaattatgcg gaagacgcca
     3361 agaaattggt tgagacgcta ccaagttcgt acccctctcg tgaatatttt gttgaaccta
     3421 atagtgccga aggaaaaaca acaattaatg tgtttgttac cggtgtcaca ggatttctgg
     3481 gctcctacat ccttgcagat ttgttaggac gttctccaaa gaactacagt ttcaaagtgt
     3541 ttgcccacgt cagggccaag gaggatgaag aagctgcatt tgcaagatta caaaaggcag
     3601 gtatcaccta tggtacttgg aacgaaaaat ttgcctcaaa tattaaagtt gtattaggcg
     3661 atttatctaa aagccaattt ggtctttcag atgagaagtg gatggatttg gcaaacacag
     3721 ttgatataat tatccataat ggtgcgttag ttcactgggt ttatccatat gccaaattga
     3781 gggacccaaa tgttatttca actatcaatg ttatgagctt agccgccgtc ggcaagccaa
     3841 agttctttga ctttgtttcc tccacttcta ctcttgacac tgaatactac tttaatttgt
     3901 cagataaact tgttagcgaa gggaagccag gcattttaga atcagacgat ttaatgaact
     3961 ctgcaagcgg gctcactggt ggatatggtc agtccaaatg ggctgctgag tacatcatta
     4021 gacgtgcagg tgaaaggggc ctacgtgggt gtattgtcag accaggttac gtaacaggtg
     4081 cctctgccaa tggttcttca aacacagatg atttcttatt gagatttttg aaaggttcag
     4141 tccaattagg taagattcca gatatcgaaa attccgtgaa tatggttcca gtagatcatg
     4201 ttgctcgtgt tgttgttgct acgtctttga atcctcccaa agaaaatgaa ttggccgttg
     4261 ctcaagtaac gggtcaccca agaatattat tcaaagacta cttgtatact ttacacgatt
     4321 atggttacga tgtcgaaatc gaaagctatt ctaaatggaa gaaatcattg gaggcgtctg
     4381 ttattgacag gaatgaagaa aatgcgttgt atcctttgct acacatggtc ttagacaact
     4441 tacctgaaag taccaaagct ccggaactag acgataggaa cgccgtggca tctttaaaga
     4501 aagacaccgc atggacaggt gttgattggt ctaatggaat aggtgttact ccagaagagg
     4561 ttggtatata tattgcattt ttaaacaagg ttggattttt acctccacca actcataatg
     4621 acaaacttcc actgccaagt atagaactaa ctcaagcgca aataagtcta gttgcttcag
     4681 gtgctggtgc tcgtggaagc tccgcagcag cttaaggttg agcattacgt atgatatgtc
     4741 catgtacaat aattaaatat gaattaggag aaagacttag cttcttttcg ggtgatgtca
     4801 cttaaaaact ccgagaataa tatataataa gagaataaaa tattagttat tgaataagaa
     4861 ctgtaaatca gctggcgtta gtctgctaat ggcagcttca tcttggttta ttgtagcatg
     4921 aatcatattt gccttttttt cctgtaattc aatgattctt gcttctatac tatcctcaat
     4981 gcaaaacctt gtgatcttca caggtcgata ctgaccaatt ctatgaactc tatcaccact
     5041 ttgccattca acactagggt tccaccatgg gtctaaaatg aatactcccg ggcctatgcg
     5101 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     5161 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5221 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5281 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5341 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5401 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5461 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5521 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5581 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     5641 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc
     5701 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa
     5761 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata
     5821 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg
     5881 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg
     5941 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg
     6001 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg
     6061 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt
     6121 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat
     6181 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt
     6241 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg
     6301 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
     6361 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
     6421 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat
     6481 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct
     6541 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa
     6601 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca
     6661 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
     6721 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag
     6781 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct
     6841 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
     6901 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
     6961 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
     7021 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9001 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     9061 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     9121 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     9181 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     9241 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     9301 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     9361 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     9421 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     9481 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     9541 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     9601 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     9661 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     9721 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     9781 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     9841 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     9901 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     9961 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
    10021 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
    10081 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
    10141 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
    10201 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
    10261 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
    10321 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
    10381 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
