LOCUS       sequence-44556-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O'Hanlon D, 
            Sung HK, Alvarez M, Talukder S, Pan Q, Mazzoni EO, Nedelec S, 
            Wichterle H, Woltjen K, Hughes TR, Zandstra PW, Nagy A, Wrana JL, 
            Blencowe BJ
  TITLE     An alternative splicing switch regulates embryonic stem cell 
            pluripotency and reprogramming.
  JOURNAL   Cell. 2011 Sep 30;147(1):132-46. Epub 2011 Sep 15.
  PUBMED    21924763
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-30"; volume: "147(1)"; pages: "132-46. Epub 2011 Sep 
            15"
COMMENT     Sequence Label: pLKO.1_sh_ex13
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    121..238
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        287..770
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     716..734
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tatcttgtgg aaggacgaaa caccgggctt acctcatact ccaacaactc gagttgttgg
       61 agtatgaggt aagctttttg aattctcgac ctcgagacaa atggcagtat tcatccacaa
      121 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      181 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      241 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
      301 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      361 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
      421 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
      481 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
      541 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
      601 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
      661 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
      721 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
      781 ccg
//
