LOCUS       sequence-445560-       10448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of two genes in yeast with V5 and HA tags and LYS2 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Dong G, Nakai T, Matsuzaki T
  TITLE     A novel plasmid-based experimental system in Saccharomyces 
            cerevisiae that enables the introduction of 10 different plasmids 
            into cells.
  JOURNAL   FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893.
  PUBMED    39387713
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Open 
            Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893."
COMMENT     Sequence Label: pESC-LYS (V5 and HA)
FEATURES             Location/Qualifiers
     source          1..10448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        294..533
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             534..4715
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKEDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQ
                     FGLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDF
                     VSSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAG
                     ERGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVAR
                     VVVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVI
                     DRNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEV
                     GIYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     rep_origin      complement(5147..5602)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5284..5305)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5496..5515
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(5681..5846)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5767..5791
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5991..6032)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        6085..6749
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    6301..6418
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     6713..6736
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     6760..6779
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6760..6778
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6802..6828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      6855..7044
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7116..7133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7287..7875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7367..7386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8046..8837,8838..8906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8669..8688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8907..9011)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9038..10380
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10001..10048)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10421..10439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccag cttttcaatt caattcagcc agcggaattc cacttacagc ttttcaattc
      241 aattctgcca gcggaattcc acttacagct tttcaattca attcagccag cggaattcca
      301 cttgcaatta cataaaaaat tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct
      361 gcctaatgaa catgaacatc gcccaaatgt atttgaagac ccgctgggag aagttcaaga
      421 tatataagta acaagcagcc aatagtataa aaaaaaatct gagtttatta cctttcctgg
      481 aatttcagtg aaaaactgct aattatagag agatatcaca gagttactca ctaatgacta
      541 acgaaaaggt ctggatagag aagttggata atccaactct ttcagtgtta ccacatgact
      601 ttttacgccc acaacaagaa ccttatacga aacaagctac atattcgtta cagctacctc
      661 agctcgatgt gcctcatgat agtttttcta acaaatacgc tgtcgctttg agtgtatggg
      721 ctgcattgat atatagagta accggtgacg atgatattgt tctttatatt gcgaataaca
      781 aaatcttaag attcaatatt caaccaacgt ggtcatttaa tgagctgtat tctacaatta
      841 acaatgagtt gaacaagctc aattctattg aggccaattt ttcctttgac gagctagctg
      901 aaaaaattca aagttgccaa gacctggaaa ggacccctca gttgttccgt ttggcctttt
      961 tggaaaacca agatttcaaa ttagacgagt tcaagcatca tttagtggac tttgctttga
     1021 atttggatac cagtaataat gcgcatgttt tgaacttaat ttataacagc ttactgtatt
     1081 cgaatgaaag agtaaccatt gttgcggacc aatttactca atatttgact gctgcgctaa
     1141 gcgatccatc caattgcata actaaaatct ctctgatcac cgcatcatcc aaggatagtt
     1201 tacctgatcc aactaagaac ttgggctggt gcgatttcgt ggggtgtatt cacgacattt
     1261 tccaggacaa tgctgaagcc ttcccagaga gaacctgtgt tgtggagact ccaacactaa
     1321 attccgacaa gtcccgttct ttcacttatc gcgacatcaa ccgcacttct aacatagttg
     1381 cccattattt gattaaaaca ggtatcaaaa gaggtgatgt agtgatgatc tattcttcta
     1441 ggggtgtgga tttgatggta tgtgtgatgg gtgtcttgaa agccggcgca accttttcag
     1501 ttatcgaccc tgcatatccc ccagccagac aaaccattta cttaggtgtt gctaaaccac
     1561 gtgggttgat tgttattaga gctgctggac aattggatca actagtagaa gattacatca
     1621 atgatgaatt ggagattgtt tcaagaatca attccatcgc tattcaagaa aatggtacca
     1681 ttgaaggtgg caaattggac aatggcgagg atgttttggc tccatatgat cactacaaag
     1741 acaccagaac aggtgttgta gttggaccag attccaaccc aaccctatct ttcacatctg
     1801 gttccgaagg tattcctaag ggtgttcttg gtagacattt ttccttggct tattatttca
     1861 attggatgtc caaaaggttc aacttaacag aaaatgataa attcacaatg ctgagcggta
     1921 ttgcacatga tccaattcaa agagatatgt ttacaccatt atttttaggt gcccaattgt
     1981 atgtccctac tcaagatgat attggtacac cgggccgttt agcggaatgg atgagtaagt
     2041 atggttgcac agttacccat ttaacacctg ccatgggtca attacttact gcccaagcta
     2101 ctacaccatt ccctaagtta catcatgcgt tctttgtggg tgacatttta acaaaacgtg
     2161 attgtctgag gttacaaacc ttggcagaaa attgccgtat tgttaatatg tacggtacca
     2221 ctgaaacaca gcgtgcagtt tcttatttcg aagttaaatc aaaaaatgac gatccaaact
     2281 ttttgaaaaa attgaaagat gtcatgcctg ctggtaaagg tatgttgaac gttcagctac
     2341 tagttgttaa caggaacgat cgtactcaaa tatgtggtat tggcgaaata ggtgagattt
     2401 atgttcgtgc aggtggtttg gccgaaggtt atagaggatt accagaattg aataaagaaa
     2461 aatttgtgaa caactggttt gttgaaaaag atcactggaa ttatttggat aaggataatg
     2521 gtgaaccttg gagacaattc tggttaggtc caagagatag attgtacaga acgggtgatt
     2581 taggtcgtta tctaccaaac ggtgactgtg aatgttgcgg tagggctgat gatcaagtta
     2641 aaattcgtgg gttcagaatc gaattaggag aaatagatac gcacatttcc caacatccat
     2701 tggtaagaga aaacattact ttagttcgca aaaatgccga caatgagcca acattgatca
     2761 catttatggt cccaagattt gacaagccag atgacttgtc taagttccaa agtgatgttc
     2821 caaaggaggt tgaaactgac cctatagtta agggcttaat cggttaccat cttttatcca
     2881 aggacatcag gactttctta aagaaaagat tggctagcta tgctatgcct tccttgattg
     2941 tggttatgga taaactacca ttgaatccaa atggtaaagt tgataagcct aaacttcaat
     3001 tcccaactcc caagcaatta aatttggtag ctgaaaatac agtttctgaa actgacgact
     3061 ctcagtttac caatgttgag cgcgaggtta gagacttatg gttaagtata ttacctacca
     3121 agccagcatc tgtatcacca gatgattcgt ttttcgattt aggtggtcat tctatcttgg
     3181 ctaccaaaat gatttttacc ttaaagaaaa agctgcaagt tgatttacca ttgggcacaa
     3241 ttttcaagta tccaacgata aaggcctttg ccgcggaaat tgacagaatt aaatcatcgg
     3301 gtggatcatc tcaaggtgag gtcgtcgaaa atgtcactgc aaattatgcg gaagacgcca
     3361 agaaattggt tgagacgcta ccaagttcgt acccctctcg tgaatatttt gttgaaccta
     3421 atagtgccga aggaaaaaca acaattaatg tgtttgttac cggtgtcaca ggatttctgg
     3481 gctcctacat ccttgcagat ttgttaggac gttctccaaa gaactacagt ttcaaagtgt
     3541 ttgcccacgt cagggccaag gaggatgaag aagctgcatt tgcaagatta caaaaggcag
     3601 gtatcaccta tggtacttgg aacgaaaaat ttgcctcaaa tattaaagtt gtattaggcg
     3661 atttatctaa aagccaattt ggtctttcag atgagaagtg gatggatttg gcaaacacag
     3721 ttgatataat tatccataat ggtgcgttag ttcactgggt ttatccatat gccaaattga
     3781 gggacccaaa tgttatttca actatcaatg ttatgagctt agccgccgtc ggcaagccaa
     3841 agttctttga ctttgtttcc tccacttcta ctcttgacac tgaatactac tttaatttgt
     3901 cagataaact tgttagcgaa gggaagccag gcattttaga atcagacgat ttaatgaact
     3961 ctgcaagcgg gctcactggt ggatatggtc agtccaaatg ggctgctgag tacatcatta
     4021 gacgtgcagg tgaaaggggc ctacgtgggt gtattgtcag accaggttac gtaacaggtg
     4081 cctctgccaa tggttcttca aacacagatg atttcttatt gagatttttg aaaggttcag
     4141 tccaattagg taagattcca gatatcgaaa attccgtgaa tatggttcca gtagatcatg
     4201 ttgctcgtgt tgttgttgct acgtctttga atcctcccaa agaaaatgaa ttggccgttg
     4261 ctcaagtaac gggtcaccca agaatattat tcaaagacta cttgtatact ttacacgatt
     4321 atggttacga tgtcgaaatc gaaagctatt ctaaatggaa gaaatcattg gaggcgtctg
     4381 ttattgacag gaatgaagaa aatgcgttgt atcctttgct acacatggtc ttagacaact
     4441 tacctgaaag taccaaagct ccggaactag acgataggaa cgccgtggca tctttaaaga
     4501 aagacaccgc atggacaggt gttgattggt ctaatggaat aggtgttact ccagaagagg
     4561 ttggtatata tattgcattt ttaaacaagg ttggattttt acctccacca actcataatg
     4621 acaaacttcc actgccaagt atagaactaa ctcaagcgca aataagtcta gttgcttcag
     4681 gtgctggtgc tcgtggaagc tccgcagcag cttaaggttg agcattacgt atgatatgtc
     4741 catgtacaat aattaaatat gaattaggag aaagacttag cttcttttcg ggtgatgtca
     4801 cttaaaaact ccgagaataa tatataataa gagaataaaa tattagttat tgaataagaa
     4861 ctgtaaatca gctggcgtta gtctgctaat ggcagcttca tcttggttta ttgtagcatg
     4921 aatcatattt gccttttttt cctgtaattc aatgattctt gcttctatac tatcctcaat
     4981 gcaaaacctt gtgatcttca caggtcgata ctgaccaatt ctatgaactc tatcaccact
     5041 ttgccattca acactagggt tccaccatgg gtctaaaatg aatactcccg ggcctatgcg
     5101 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     5161 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5221 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5281 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5341 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5401 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5461 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5521 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5581 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     5641 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc
     5701 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa
     5761 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata
     5821 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg
     5881 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg
     5941 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca ggtagaatcc
     6001 agacccagca gagggttagg gataggctta ccagatctca tcgatactag tgcggccgcc
     6061 ctttagtgag ggttgaattc gaattttcaa aaattcttac tttttttttg gatggacgca
     6121 aagaagttta ataatcatat tacatggcat taccaccata tacatatcca tatacatatc
     6181 catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc ttagcctaaa
     6241 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta
     6301 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
     6361 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
     6421 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
     6481 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
     6541 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
     6601 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
     6661 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
     6721 tctatacttt aacgtcaagg agaaaaaacc ccggatccgt aatacgactc actatagggc
     6781 ccgggcgtcg acatgctcga gtacccatac gatgttccag attacgctta agcttggtac
     6841 cgcggctagc taagatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     6901 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     6961 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     7021 gagaaggttt tgggacgctc gaagatccag ctgcattaat gaatcggcca acgcgcgggg
     7081 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7141 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7201 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7261 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7321 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7381 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7441 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7501 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7561 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7621 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7681 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7741 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7801 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7861 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7921 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7981 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8041 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8101 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8161 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8221 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8281 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8341 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8401 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8461 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8521 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8581 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8641 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8701 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8761 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8821 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8881 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8941 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9001 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt
     9061 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     9121 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     9181 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     9241 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     9301 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     9361 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     9421 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     9481 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     9541 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     9601 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     9661 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     9721 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     9781 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     9841 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     9901 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     9961 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
    10021 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
    10081 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
    10141 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
    10201 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
    10261 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
    10321 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
    10381 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10441 ctttcgtc
//
