LOCUS       sequence-445605-        7366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpL and GvpU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaLU
FEATURES             Location/Qualifiers
     source          1..7366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5624..5642)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5638..5685
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5752..5843
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6708..6747
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6778..7366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7267..7286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagagtgag gaattaacat tatgtctctt aaacaatcca tggagaataa
     3781 agatattgct cttattgata ttttagatgt cattttagat aaaggagtcg ccattaaagg
     3841 agacttaatc atttccatag ctggcgtcga tttagtgtat ttggatttgc gggtgcttat
     3901 ttcttcggtt gaaacgcttg tgcaagcaaa agaaggaaat cacaaaccaa tcacttctga
     3961 acaatttgat aaacaaaagg aggaattaat ggatgcaacc ggtcagccaa gcaaatggac
     4021 gaatccactt ggatcctgat caagctgaac aaggcttagc gcagcttgtg atgacagtta
     4081 ttgagctatt gaggcaaatc gttgaacgtc atgccatgag gcgggtggag ggtggaacgt
     4141 tgacggacga acaaattgaa aacttaggaa ttgcactaat gaacttagaa gaaaaaatgg
     4201 acgagttgaa agaggtgttc ggtctggatg cagaagattt aaatattgat cttggaccgc
     4261 taggcagcct gctttaagcg gtcagtagga ggaacagtat ggcagtcgaa cataatatgc
     4321 agtcaagtac gattgtagat gtgctcgaaa agattttgga taaaggagtc gttatagcgg
     4381 gggacatcac cgtaggaatt gcagatgtcg agctattaac gataaagatc cgcttgattg
     4441 tggcttcggt tgataaggca aaagaaatcg gcatggactg gtgggaaaat gatccgtatc
     4501 tcagttcaaa aggagccaat aacaaagcgc tcgaagaaga aaataaaatg ctgcatgagc
     4561 ggttaaaaac gcttgaagaa aaaatagaaa cgaaacgtta aaaactgtac gctacttaaa
     4621 aaatggaggg atttacaatg gcaactgaaa caaaattaga taacacacag gcagaaaaca
     4681 aggaaaataa aaatgcggaa aacggttcaa aagaaaagaa cggttcaaaa gcaagcaaaa
     4741 caacaagcag cgggccaatc aaacgagcgg tagcaggagg catcatcggt gcaacgattg
     4801 gatatgtatc gactcctgaa aatcgaaaaa gtctccttga ccgcattgat acagacgaat
     4861 taaaaagcaa agcatctgat ttaggaacaa aggtaaaaga aaaatcaaaa agcagcgtgg
     4921 ccagcctgaa aacatctgcg ggaagcttgt ttaaaaaaga taaagataaa tcaaaagatg
     4981 atgaagaaaa cgtaaattct tctagtagcg aaacagaaga cgataacgtt caagagtacg
     5041 acgagttaaa agaagaaaat caaactcttc aagatcgctt atcacagctt gaagaaaaaa
     5101 tgaacatgct tgttgagctt agcctcaata aaaatcaaga cgaagaagcg gaagatacag
     5161 attccgacga agaagagaac gatgagaacg atgaaaacga tgaaaacgag caggacgatg
     5221 aaaacgaaga agaaacatct aagccacgta aaaaggataa aaaagaagct gaggaagaag
     5281 aaagtgaaag tgacgaagac agcgaggaag aagaggaaga ttctcgctca aacaaaaaaa
     5341 ataaaaaagt aaaaacagaa gaagaagacg aagatgaaag cgaagaagaa aaaaaggaag
     5401 cgaaaccaaa aaagtcaaca gctaaaaaat caaaaaatac aaaagcaaag aaaaacacgg
     5461 acgaagaaga tgatgaagca acatctcttt ctagtgaaga cgatacaaca gcctaagacg
     5521 tcgtcacatt tgcttctgct tgctgcagtc cggattcgcg aacgcgtctc gagagatccg
     5581 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     5641 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     5701 atatccgact agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
     5761 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5821 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5881 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctcgtg aaagtaaaag
     5941 acgcagagga ccaattgggg gcacgagtgg gatacataga actggacttg aatagcggta
     6001 aaatccttga gagttttcgc cctgaagagc gttttccaat gatgagcact ttcaaagttc
     6061 tgctatgtgg agcagtatta tcccgtgtag atgcggggca agagcaactc ggacgacgaa
     6121 tacactattc gcagaatgac ttggttgaat actccccagt gacagaaaag caccttacgg
     6181 acggaatgac ggtaagagaa ttatgtagtg ccgccataac gatgagtgat aacactgcgg
     6241 cgaacttact tctgacaacc atcggtggac cgaaggaatt aaccgctttt ttgcacaata
     6301 tgggagacca tgtaactcgc cttgaccgtt gggaaccaga actgaatgaa gccataccaa
     6361 acgacgagcg agacaccaca atgcctgcgg caatggcaac aacattacgc aaactattaa
     6421 ctggcgaact acttactctg gcttcacggc aacaattaat agactggctt gaagcggata
     6481 aagttgcagg accactactg cgttcggcac ttcctgctgg ctggtttatt gctgataaat
     6541 ctggggcagg agagcgtggt tcacggggta tcattgccgc acttggacca gatggtaagc
     6601 cttcccgtat cgtagttatc tacacgacgg gtagtcaggc aactatggac gaacgaaata
     6661 gacagattgc tgaaataggg gcttcactga ttaagcattg gtaaaccgat acaattaaag
     6721 gctccttttg gagccttttt ttttggacgg accggtagaa aagatcaaag gatcttcttg
     6781 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6841 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6901 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6961 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7021 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7081 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7141 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7201 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7261 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7321 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaa
//
